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- 저자 : Styrak, Igor (검색결과 1 건)
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Jung, Min Young,Kim, Joong-Su,Paek, Woon Kee,Styrak, Igor,Park, In-Soon,Sin, Yeseul,Paek, Jayoung,Park, Keun Ae,Kim, Hongik,Kim, Hong Lim,Chang, Young-Hyo Microbiology Society 2012 International journal of systematic and evolutiona Vol.62 No.10
<P>A Gram-positive, rod-shaped, endospore-forming bacterium, designated strain BLB-1<SUP>T</SUP>, was isolated from samples of tidal flat sediment from the Yellow Sea. 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the <I>Bacillus</I> rRNA group 2 and was closely related to <I>Bacillus massiliensis</I> CIP 108446<SUP>T</SUP> (97.4 %), <I>Bacillus odysseyi</I> ATCC PTA-4993<SUP>T</SUP> (96.7 %), <I>Lysinibacillus fusiformis</I> DSM 2898<SUP>T</SUP> (96.2 %) and <I>Lysinibacillus boronitolerans</I> DSM 17140<SUP>T</SUP> (95.9 %). Sequence similarities with related species in other genera, including <I>Caryophanon</I>,<I/><I>Sporosarcina</I> and <I>Solibacillus</I>, were <96.1 %. Chemotaxonomic data supported the affiliation of strain BLB-1<SUP>T</SUP> with the genus <I>Lysinibacillus</I>. The major menaquinone was MK-7, the cell-wall sugars were glucose and xylose, the cell-wall peptidoglycan type was A4α (l-Lys-d-Asp), the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown phospholipids, and the major fatty acids were anteiso-C15 : 0 (35.6 %), iso-C15 : 0 (25.6 %) and anteiso-C17 : 0 (16.5 %). The most closely related species, <I>Bacillus massiliensis</I> and <I>Bacillus odysseyi</I>, were also assigned to this genus based on phylogenetic analysis and phenotypic data. The results of DNA-DNA hybridizations and phenotypic tests supported the differentiation of all three taxa from species of the genus <I>Lysinibacillus</I> with validly published names. Thus, strain BLB-1<SUP>T</SUP> ( = KCTC 13296<SUP>T</SUP> = JCM 15800<SUP>T</SUP>) represents a novel species, for which the name <I>Lysinibacillus sinduriensis</I> sp. nov. is proposed. It is also proposed that <I>Bacillus massiliensis</I> CIP 108446<SUP>T</SUP> ( = 4400831<SUP>T</SUP> = CCUG49529<SUP>T</SUP> = KCTC 13178<SUP>T</SUP>) and <I>Bacillus odysseyi</I> NBRC 100172<SUP>T</SUP> ( = 34hs-1<SUP>T</SUP> = ATCC PTA-4993<SUP>T</SUP> = NRRL B-30641<SUP>T</SUP> = DSM 18869<SUP>T</SUP> = CIP 108263<SUP>T</SUP> = KCTC 3961<SUP>T</SUP>) be transferred to the genus <I>Lysinibacillus</I> as <I>Lysinibacillus massiliensis</I> comb. nov. and <I>Lysinibacillus odysseyi</I> comb. nov., respectively.</P>
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