http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yamane, Akira,takahashi, Katsu,Bringas, Pablo,Amano, Osamu,Slavkin, Harold C.,Margarita, Zeichner-David Korean Academy of Oral Biology and the UCLA Dental 1997 International Journal of Oral Biology Vol.22 No.3
In order to elucidate the roles of insulin, IGF-Ⅰ and Ⅱ during amelogenesis, the effects of these factors on the translational activity of amelogenin, the predominant matrix protein in developing enamel, were examined in mouse embryonic molars maintained in vitro. Mouse mandibular first molars isolated from embryos on embryonic day 15 were cultured as an explant for 6, 12 and 18 days in serumless, chemically defined medium containing 1000ng/ml of insulin, 100ng/ml of IGF-Ⅰ or 100ng/ml of IGF-Ⅱ. The translational activity of amelogenin was measured by incorporation of [^35S]-methionine, immunoprecipitation with a specific amelogenin antibody followed by polyacrylamide gel electrophoresis in combination with fluorography. IGF-Ⅰ and IGF-Ⅱ induced 55% and 104% increases in the translational activity of amelogenin respectively at 6 days in culture. This effect was lost after 12 and 18 days in culture. Insulin did not produce any significant differences in the translational activity of amelogenin. These data suggested that IGF-Ⅰ and Ⅱ accelerated amelogenesis by inducing an increase in amelogenin translation in the mouse mandibular first molar in vitro.