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        Anesthesia and analgesia for common research models of adult mice

        Siavash Ahmadi-Noorbakhsh,Mohammad Farajli Abbasi,Maedeh Ghasemi,Gholamreza Bayat,Nahid Davoodian,Ehsan Sharif-Paghaleh,Seyedeh Mahsa Poormoosavi,Melika Rafizadeh,Maryam Maleki,Hesamaddin Shirzad-Aski 한국실험동물학회 2022 Laboratory Animal Research Vol.38 No.4

        Anesthesia and analgesia are major components of many interventional studies on laboratory animals. However, various studies have shown improper reporting or use of anesthetics/analgesics in research proposals and published articles. In many cases, it seems “anesthesia” and “analgesia” are used interchangeably, while they are referring to two different concepts. Not only this is an unethical practice, but also it may be one of the reasons for the proven suboptimal quality of many animal researches. This is a widespread problem among investigations on various species of animals. However, it could be imagined that it may be more prevalent for the most common species of laboratory animals, such as the laboratory mice. In this review, proper anesthetic/analgesic methods for routine procedures on laboratory mice are discussed. We considered the available literature and critically reviewed their anesthetic/analgesic methods. Detailed dosing and pharmacological information for the relevant drugs are provided and some of the drugs’ side effects are discussed. This paper provides the necessary data for an informed choice of anesthetic/analgesic methods in some routine procedures on laboratory mice.

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        A proposed framework for holding intensive 3Rs workshops in laboratory animal science

        Siavash Ahmadi-Noorbakhsh,Jila Sadighi,Zahra Hatami,Ehsan Shamsi Gooshki 한국실험동물학회 2022 Laboratory Animal Research Vol.38 No.1

        Principles of 3Rs are the backbone of today’s animal research. Applying 3Rs in practice requires proper education and training. Although this could be achieved via long-term courses ranging from several weeks to years, in some circumstances a short-term course may be the only viable option. In this paper we define scientific and ethical objectives for running short-term 3Rs workshops. To meet these objectives, we propose a 2-day workshop curriculum and an ethical framework. The curriculum comprises theoretical and practical sessions and covers Replacement, Reduction, and Refinement strategies. The ethical framework defines the responsibilities of lecturers and attendees, and proposes the animals and facilities requirements to run the proposed workshop curriculum. The attendees will be assessed at the end of the workshop and they receive certificates for working under supervision until they are deemed competent at their intended functions. The proposed curriculum and framework are not subscriptive, instead they share the experience gained through running more than 60 laboratory animal 3Rs workshops.

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