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        Extraction of polysaccharides under vacuum condition from Lentinus edodes stipe and their antioxidant activities in vitro

        Shunfeng Li,Anjian Wang,Lina Liu,Guangrui Tian,Fangfang Xu 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3

        To improve the efficiency of polysaccharideextraction, vacuum technology was used to breakdown thecell wall to extract polysaccharides from Lentinus edodesstipe, and the optimal parameters were optimized byresponse surface methodology. Then the antioxidantactivities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharideextraction were temperature of 62 C, vacuum of0.08 MPa, stirring speed of 1200 r/min, time of 25 min andmaterial/liquid ratio of 1:26 (g/mL). Under these conditions,the yield of polysaccharide was 4.28%. The decolorizationrate and deproteinization rate of polysaccharidestreated by kaolin and hydrogen peroxide were higher thanthat of activated carbon. However, there was no significantinfluence on the polysaccharide retention rate, that wasabout 80%. Results also indicated that polysaccharidetreated with activated carbon could obtain higher antioxidantactivity.

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        Sodium alginate-based wall materials microencapsulated Lactobacillus plantarum CICC 20022: characteristics and survivability study

        Lihua Zhang,Peixin Tang,Shunfeng Li,Xia Wang,Wei Zong 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.11

        In this study, microcapsules of Lactobacillus plantarum CICC 20022 (L. plantarum CICC 20022) were prepared by extrusion technique using sodium alginate (SA), sodium alginate-sodium caseinate (SA-SC) and sodium alginate-whey protein isolate (SA-WPI) as wall materials, respectively. Results showed that the best encapsulation yield of L. plantarum CICC 20022 was SA-WPI. Morphology and texture analysis showed that the microcapsules prepared by the SA-WPI system presented a more compact internal structure and higher resistance to external pressure. Fourier-transform infrared spectroscopy and low field nuclear magnetic resonance analysis demonstrated that the hydrogen bonding ability and network structure of the SA were improved by the addition of WPI. The survivability of L. plantarum CICC 20022 entrapped with the SA-WPI system was improved during freeze-drying and simulated gastrointestinal digestion. Therefore, the SA-WPI system can potentially be used as the vector of L. plantarum CICC 20022 in food applications.

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