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Medical Image Segmentation Based on Morphology Algorithm and FCM Algorithm
Shigang Wang,Zhinan Rong,Xueshan Gao 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10
Fuzzy c-means algorithm is an unsupervised clustering algorithm, its clustering process can reduce the human intervention, and it is suitable for processing medical images of uncertainty and ambiguity. When simply using FCM algorithm in brain image segmentation will leads to the condition of low accuracy. On the basis of FCM algorithm, this paper proposes a new method which combines FCM algorithm and morphology algorithm. The result of simulation shows that this method can accurately and efficiently segment the brain image. The new algorithm is an effective method for image segmentation.
Liu, Xin,Zeng, Rong,Gao, Shigang,Xu, Lihui,Dai, Fuming The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.1
$SdhB^{P225F}$ and $SdhB^{H272R}$ mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for $SdhB^{P225F}$ and $SdhB^{H272R}$ were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by $SdhB^{P225F}$ and $SdhB^{H272R}$ mutations.
Xin Liu,Rong Zeng,Shigang Gao,Lihui Xu,Fuming Dai 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.1
SdhBP225Fand SdhBH272R mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for SdhBP225F and SdhBH272R were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by SdhBP225F and SdhBH272R mutations.
( Lifang Yue ),( Hexin Lv ),( Jing Zhen ),( Shengping Jiang ),( Shiru Jia ),( Shigang Shen ),( Lu Gao ),( Yujie Dai ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.