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RISS 인기검색어
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- 저자 : Shenglin Wang (검색결과 4 건)
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Shenglin Hu,Dongmei Wang,Jiong Hong 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.1
In this study, a simple, inexpensive and fast β-glucosidase immobilization system was constructed and evaluated in isoflavone glycosides hydrolysis. A β-glucosidase gene from Thermoascus aurantiacus IFO9748 was recombinantly expressed in Pichia pastoris KM71H and immobilized on regenerated amorphous cellulose (RAC) by fused cellulose binding module 3. Through simple mixing cellulose and crude enzyme for 15 min under room temperature, 96.04% β-glucosidase was immobilized onto RAC. The optimum temperature for β-glucosidase activity was increased by 5ºC after immobilization. The half-life (t½) of heat inactivation of immobilized enzyme at 60oC was improved over 8 folds. After 30 rounds recycled at 40oC, 96.9% daidzin and 98.9% genistin could still be hydrolyzed. A continuous hydrolysis system was also constructed, and at the flow rate of 0.2 mL/min after 30 h hydrolysis, 95.6% genistin and 90.2% daidzin can still be hydrolyzed. Combined the simple and high efficient enzyme immobilization procedure and inexpensive cellulose, this scalable and practical system may have broad prospects for industrial utilization.
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Zhian Jin,Pujie Wang,Jie Chen,Li He,Lijia Xiao,Kaisen Yong,Shenglin Deng,Lin Zhou 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.5
Purpose: C-end rule (CendR) peptides are found to enhance the penetration of chemotherapeutic agents into tumor cells, whileGX1 is a peptide that homes to gastric cancer (GC) vasculature. This study aimed to synthesize a novel peptide GX1-RPAKPAR(GXC) and to explore the effect of GXC on sensitizing GC cells to chemotherapeutic agents. Materials and Methods: Intracellular Adriamycin concentration analysis was applied to conform whether GXC peptide increasesthe penetration of chemotherapeutic agents into GC cells in vitro. The effect of GXC peptide on sensitizing GC cells to chemotherapeuticswas validated by apoptosis assay and in vitro/vivo drug sensitivity assay. The specificity of GXC to GC tissue was validatedby ex vivo fluorescence imaging. Results: In vitro, administration of GXC significantly increased Adriamycin concentrations inside SGC-7901 cells, and enhancedthe efficacy of chemotherapeutic agents by decreasing the IC50 value. In vivo, FITC-GXC specifically accumulated in GC tissue. Moreover, systemic co-injection with GXC peptide and Adriamycin statistically improved the therapeutic efficacy in SGC-7901xenograft models, surprisingly, without obviously increasing side effects. Conclusion: These results demonstrated that co-administration of the novel peptide GXC with chemotherapeutic agents may bea potential way to enhance the efficacy of anticancer drugs in GC treatment.
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Levosimendan Reduces Lung Injury in a Canine Model of Cardiopulmonary Bypass
Junbo Feng,Haiyuan Liu,Jiayi Chen,Jiyuan Wang,Zhuang Liu,Shenglin Ge 대한심장학회 2016 Korean Circulation Journal Vol.46 No.3
Background and Objectives: To explore the lung-protective effect of levosimendan (LS) during cardiopulmonary bypass in a canine modelby determining the wet/dry weight (W/D) ratio of lung tissue, malonaldehyde (MDA) and superoxide dismutase (SOD) concentrations, andperforming a histological evaluation. Materials and Methods: Thirty-two canines were divided randomly into four groups and underwent a routine aortic cross-clampingcardiopulmonary bypass procedure for 1 h, followed by recovery for 2 h. Animals were handled as follows: group C (means controlgroup), no special treatment after aortic cross clamping; group P (means pulmonary artery perfusion group), pulmonary artery perfusionwith cold oxygenated blood after aortic cross clamping; group LSIV (means intravenous injection of LS group), intravenous injection of LS(65 μg/kg) before thoracotomy, and the rest of the procedure was identical to the control group; group LPS (means pulmonary perfusionwith LS group), pulmonary perfusion with cold oxygenated blood combined with LS (65 μg/kg) after aortic cross clamping. Lung tissueswere removed and subjected to evaluation of pathological alterations, W/D ratio and MDA and SOD concentrations. Results: In group C, the W/D ratio and MDA concentration were higher, while the SOD concentrations were lower (p<0.05). Comparedwith groups P and LSIV, the MDA concentration was lower in group LPS, while that of SOD was higher (p<0.05); Light and electronmicroscopy indicated that LS intervention reduced impairment of lung tissues. Conclusion: Our findings suggest that LS plays an important role in protecting lung tissues.
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Yinglun Tian,Nanfang Xu,Ming Yan,Jinguo Chen,Kan-Lin Hung,Xiangyu Hou,Shenglin Wang,Weishi Li 대한척추신경외과학회 2021 Neurospine Vol.18 No.4
Objective: To summarize the vertebral artery (VA) pattern of 96 “sandwich” atlantoaxial dislocation (AAD) patients and to describe the strategies of reducing the injury of VA during surgery. Methods: From 2009 to 2020, we retrospectively reviewed the 3-dimensional computed tomography angiography data of 96 AAD patients combined with atlas occipitalization and C2–3 fusion, which were diagnosed as “sandwich” AAD and 96 patients as control group patients who were without atlas occipitalization, C2–3 fusion and any other cervical bone deformity at our institution. The variations of each side of VA were described in 3 different parts (C0–1, C1–2, and C2–3) according to the characteristics of the 3-part pathological structures in “sandwich” subgroup. Results: One hundred ninety-two sides of VAs in every group of patients were analyzed and every VA was described separately at 3 different level regions. There were different variations in these 3 different regions: 4 variations in the upper fusion region, 5 variations in the sandwiched region, and 6 variations in the lower fusion region in sandwich AAD patients. And the rate of VA deformity in sandwich AAD patients was much higher and more types of VA variations existed. Conclusion: In “sandwich” AAD patients, deformities of vertebral arteries in craniovertebral junction are more common, and the same VA may have deformities at different levels that severely affect surgical procedures. Therefore, preoperative imaging examination of VA for “sandwich” AAD patients is vital of guiding surgeons to avoid injury of VA during surgery.
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