Semaphoring mAb: a New Guide in RIT in Inhibiting the Proliferation of Human Skin Carcinoma

Liu, Yuan,Ma, Jing-Yue,Luo, Su-Ju,Sun, Chen-Wei,Shao, Li-Li,Liu, Quan-Zhong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Semaphoring is a transmembrane receptor which participates in many cytokine-mediated signal pathways that are closely related to the angiogenesis, occurrence and development of carcinoma. The present study was designed to access the effect of mono-antibody (mAb) guided radioimmunotherapy (RIT) on skin carcinoma and investigate the potential mechanisms. Semaphoring mAb was acquired from mice (Balb/c), purified with rProtein A column; purity, concentration and activity were tested with SDS-PAGE and indirect ELISA; specificity and expression on the cutanuem carcinoma line and tissue were tested by Western blotting; morphology change was assessed by microscopy. MTT assay and colony inhibition tests were carried out to test the influence on the proliferation of tumor cells; Western blotting was also carried out for expression of apoptosis-associated (caspase-3, Bax, Bcl-2) and proliferation-related (PI3K, p-Akt, Akt, p-ERK1/2, ERK1/2) proteins and analyse the change in signal pathways (PI3K/Akt and MEK/ERK). The purity of purified semaphorin mAb was 96.5% and the titer is about $1{\times}10^6$. Western blotting showed semaphoring mAb to have specifically binding stripes with semaphoring b1b2 protein, B16F10, and A431 cells at 39KDa, 100KDa and 130KDa, respectively. Positive expression was detected both in cutanuem carcinoma line and tissue and it mostly located in cell membranes. MMT assay revealed dose-relate and time-relate inhibitory effect of semaphorin mAb on A431 and B16F10. Colony inhibition tests also showed dose-relate inhibitory effects. Western blotting demonstrated the expression of apoptosis and proliferation-related protein and changes in signal pathway. In conclusion, we demonstrated that semaphorin is highly expressed on the tumor cell-surfaces and RIT with semaphorin mAb has effect in i nhibiting proliferation and accelerating apoptosis of tumor cells.

Prevalence of Human Papillomavirus 16 in Esophageal Cancer Among the Chinese Population: a Systematic Review and Meta-analysis

Zhang, Shao-Kai,Guo, Lan-Wei,Chen, Qiong,Zhang, Meng,Liu, Shu-Zheng,Quan, Pei-Liang,Lu, Jian-Bang,Sun, Xi-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background and Aim: No firm evidence of HPV infection in esophageal cancer has been established to date. The aim of this meta-analysis was to investigate the prevalence of HPV 16 in esophageal cancer in China, which had a high burden of the disease. Materials and Methods: Studies on HPV infection and esophageal cancer were identified and a random-effects model was used to pool the summary prevalence and corresponding 95% confidence intervals (CIs). Results: A total of 3,429 esophageal cancer cases were evaluated from 26 eligible studies in this meta-analysis. The summary estimate for HPV16 prevalence was 0.381 (95% CI: 0.283, 0.479). The prevalence varied by geographical areas of the study, publication year, HPV detection method and types of specimen. In sensitivity analysis, HPV 16 prevalence ranged from 0.368 (95% CI: 0.276, 0.460) to 0.397 (95% CI: 0.286, 0.508). Conclusions: The results indicate a relatively high level of HPV 16 prevalence in esophageal cancer among Chinese population, although there was variation between different variables. Further studies are needed to elucidate the role of HPV in esophageal carcinogenesis with careful consideration of study design and laboratory detection method, providing more accurate assessment of the HPV status in esophageal cancer.

Deployment Dynamics of Large-Scale Flexible Solar Arrays with Deployable Mast

Hai-Quan LI,Xiao-Feng LIU,Shao-Jing GUO,Guo-Ping CAI 한국항공우주학회 2017 International Journal of Aeronautical and Space Sc Vol.18 No.2

In this paper, deployment dynamics of large-scale flexible solar arrays with deployable mast is investigated. The adopted solar array system is introduced firstly, then kinematic description and kinematic constraint equations are deduced, and finally, dynamics equation of the system is established by the Jourdain velocity variation principle and a new method to deal with topology changes of the deployable mast is introduced. The dynamic behavior of the system is studied in detail. Simulation results indicate that the proposed model is effective to describe the deployment dynamics of the solar arrays and that the introduced method is applicable for topology changes.

The Prognostic Value of Treatment-Related Lymphopenia in Nasopharyngeal Carcinoma Patients

Li-Ting Liu,Qiu-Yan Chen,Lin-Quan Tang,Shan-Shan Guo,Ling Guo,Hao-Yuan Mo,Ming-Yuan Chen,Chong Zhao,Xiang Guo,Chao-Nan Qian,Mu-Sheng Zeng,Jin-Xin Bei,Jing Tan,Shuai Chen,Ming-Huang Hong,Jian-Yong Shao 대한암학회 2018 Cancer Research and Treatment Vol.50 No.1

Purpose This study was conducted to evaluate the prognostic value of treatment-related lymphopenia in patients with nasopharyngeal carcinoma (NPC). Materials and Methods A total of 413 consecutive stage II-IVb NPC patients treated with concurrent chemoradiotherapy (CCRT) were enrolled. The overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS) were calculated with the Kaplan-Meier method, and differences were compared using the log-rank test. Results A minimum (mini)–absolute lymphocyte counts (ALC) of < 390 cells/μL or ALC after 3 months of CCRT (post3m-ALC) < 705 cells/μL was significantly associated with worse outcome than mini-ALC ! 390 cells/μL (OS, p=0.002; PFS, p=0.005; DMFS, p=0.004) or post3m-ALC ! 705 cells/μL (OS, p < 0.001; PFS, p < 0.001; DMFS, p=0.001). Patients with lymphopenia (mini-ALC < 390 cells/μL and post3m-ALC < 705 cells/μL) had a worse prognosis than those without lymphopenia (mini-ALC ! 390 cells/μL and post3m-ALC ! 705 cells/μL) (OS, p < 0.001; PFS, p < 0.001; DMFS, p < 0.001). Multivariate analysis revealed that post3m-ALC was an independent prognostic factor for OS (hazard ratio [HR], 1.76; 95% confidence interval [CI], 1.12 to 2.78; p=0.015), PFS (HR, 1.86; 95% CI, 1.23 to 2.82; p=0.003), and DMFS (HR, 1.87; 95% CI, 1.13 to 3.08; p=0.014). Multivariate analysis also revealed that patients with lymphopenia had a high risk of death (HR, 3.79; 95% CI, 1.75 to 8.19; p=0.001), disease progression (HR, 2.93; 95% CI, 1.59 to 5.41; p=0.001), and distant metastasis (HR, 3.89; 95% CI, 1.67 to 9.10; p=0.002). Multivariate analysis performed with time dependent Cox regression demonstrated ALC was an independent prognostic factor for OS (HR, 0.995; 95% CI, 0.991 to 0.999; p=0.025) and PFS (HR, 0.993; 95% CI, 0.988 to 0.998; p=0.006). Conclusion Treatment-related lymphopenia was a poor prognostic factor in NPC patients.

자유주제 : 조선 사신들의 눈에 비친 明末(명말) 山東(산동)의 사회 풍속 일면 고찰 -海路(해로) 朝天日記(조천일기)와 漢詩(한시)를 중심으로-

유보전 ( Bao Quan Liu ),소쌍쌍 ( Shuang Shuang Shao ) 동방한문학회 2014 東方漢文學 Vol.0 No.61

명나라 말기에 조선 사신들은 海路와 山東을 거쳐 北京에 와서 조공하였다. 그들이 남긴 朝天錄에는 당시 명나라 사회의 남녀 복식, 머리 장식품, 여성의 화장과 纏足 등 풍속과 풍습이 여실히 기록되었다. 朝天錄에는 명나라의 복식이 지나치게 화려하고 사치스럽고 새로운 양식도 나타났다고 기록되었다. 예를 들면, 남녀 복식의 길이의 변화, ‘左.’ 양식의 유행 등은 다 재래의 유교 예법을 타파하였다는 것이다. 이는 명나라 공식적인 복식 금령이 그 구속력을 상실하고 복식 풍속이 예법에 어긋나기 시작하며 ‘胡風’을 숭상하고 화려하고 사치스러움을 추구하는 풍조가 점차 더 심해졌음을 보여 준다. 조선 사신들은 이런 현상을 조선의 그것들과 비교하면서 복식 예법이 파괴된 天朝上國인 명나라에 대한 재래 인식을 점차 바꾸기 시작하였다. 명나라 말기에는 山東의 경제생산 풍속이 다양해졌다. 상품 경제는 번창하고 배금하는 풍조가 유행하여 문인 사대부나 고위 관리들도 이익을 추구하기 위하여 상업을 많이 하였다. 登州, 濟南과 德州 등 고을에는 市肆도 많고 술집과 청루도 많아 태평성세의 모습을 나타냈다. 그렇지만 전통적 사회 윤리는 이것으로 인해 커다란 교란을 받았다. 전통적인 복식 예법도 많이 파괴되었다. 다시 말해서 복식 풍속은 상업 경제의 발전과 밀접한 관계가 있다는 것이다. In the late Ming Dynasty, the Joseon Dynasty envoys made their pilgrimage to Beijing by sea and through Shandong, with their “travel notes” presenting a faithful account of clothing, headdress, makeup, and foot-binding at that time. In that record, the Ming Dynasty clothing was of extreme floridity and extravagance, as the changes in the length of clothing and popularity in the “left lapel” style breaking the Confucian ethical code. With the official clothing restrictions in the early Ming Dynasty losing its influence, there emerged an increasingly intensified phenomenon featuring the arrogation of the clothing custom and the advocation of the “Northern style”, beauty and luxury. The Joseon envoys made comparisons between these phenomena and their national clothing culture, leading to a change in the notion that the clothing system of the Ming Dynasty was on the brink of collapse. The economic production custom was characterized by diversity in Shandong in the late Ming Dynasty, featuring the commercial prosperity, money worship and rampant pursuit of commercial profits among scholars, celebrities and high-ranking officials. Markets, taverns, and brothels could be found everywhere in cities like Dengzhou, Jinan and Dezhou. The social climate took on a scenario of prosperity and virtuous custom, while the traditional ethics were under violent strike. Under a social climate of this kind, the traditional clothing etiquette was destroyed. Therefore, the change in the clothing custom and the development of commercial economy were inextricably fused.

Deployment Dynamics of Large-Scale Flexible Solar Arrays with Deployable Mast

LI, Hai-Quan,LIU, Xiao-Feng,GUO, Shao-Jing,CAI, Guo-Ping The Korean Society for Aeronautical and Space Scie 2017 International Journal of Aeronautical and Space Sc Vol.18 No.2

In this paper, deployment dynamics of large-scale flexible solar arrays with deployable mast is investigated. The adopted solar array system is introduced firstly, then kinematic description and kinematic constraint equations are deduced, and finally, dynamics equation of the system is established by the Jourdain velocity variation principle and a new method to deal with topology changes of the deployable mast is introduced. The dynamic behavior of the system is studied in detail. Simulation results indicate that the proposed model is effective to describe the deployment dynamics of the solar arrays and that the introduced method is applicable for topology changes.

Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiang-Mei,Liu, Xing,Jiao, Qing-Fang,Fu, Shao-Yue,Bu, You-Quan,Song, Fang-Zhou,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

Research and Test on Mathematical Model of Pressure in Pump Cavity and Balance Cavity of Centrifugal Pump

Jilai Zeng,Zailun Liu,Hui Quan,Ancan Shao,Sanzhen Wang 한국유체기계학회 2022 International journal of fluid machinery and syste Vol.15 No.1

The pressure distribution law and calculation of impeller side cavity is one of the important topics in the pump industry, but there is no more accurate calculation method at present. On this basis, a mathematical model of pump cavity pressure distribution and balance cavity pressure under design conditions was derived in this paper. By changing the radial clearance of front and back sealing rings and the diameter of the balance hole on the same impeller, the pressure distribution of front and rear cavities and the balance cavity pressure of the pump were systematically tested by a specially designed device. In the test, the curve of pressure loss coefficient and relative radius of pump cavity under different sealing ring radial clearance was obtained, which could solve the calculation problem of the pressure distribution of front and rear pump cavities. The test curve of the relationship between flow coefficient ratio and specific surface area essentially reflected the coupling regulation effect of balance holes and the rear sealing ring on the balance cavity pressure, which could not only solve the calculation problem of the balance cavity pressure, but also provide the theoretical basis for controlling the pressure and cover force of balance cavity and select different combinations of the radial clearance of the rear sealing ring and the diameter of balance holes. The results showed that the theoretical prediction value was more consistent with the actual measured value, which proved that the proposed mathematical model has high accuracy and universality. This study provides a new way to more accurately calculate the pump cavity pressure distribution and balance cavity pressure and laid a theoretical basis for the study of impeller cover force with significant academic and application value.

Bioactive Properties of Novel Probiotic Lactococcus lactis Fermented Camel Sausages: Cytotoxicity, Angiotensin Converting Enzyme Inhibition, Antioxidant Capacity, and Antidiabetic Activity

Mutamed Ayyash,Amin Olaimat,Anas Al-Nabulsi,Shao-Quan Liu 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.2

Fermented products, including sausages, provide several health benefits, particularly when probiotics are used in the fermentation process. This study aimed to examine the cytotoxicity (against Caco-2 and MCF-7 cell lines), antihypertensive activity via angiotensin-converting enzyme (ACE) inhibition, antioxidant capacity, antidiabetic activity via ⍺-amylase and ⍺-glucosidase inhibition, proteolysis rate, and oxidative degradation of fermented camel and beef sausages in vitro by the novel probiotic Lactococcus lactis KX881782 isolated from camel milk. Moreover, camel and beef sausages fermented with commercial starter culture alone were compared to those fermented with commercial starter culture combined with L. lactis. The degree of hydrolysis, antioxidant capacity, cytotoxicity against Caco-2 and MCF-7, ⍺-amylase, ⍺- glucosidase, and ACE inhibitory activities were higher (p<0.05) in fermented camel sausages than beef sausages. In contrast, the water and lipid peroxidation activity were lower (p<0.05) in camel sausages than beef sausages. L. lactis enhanced the health benefits of the fermented camel sausages. These results suggest that camel sausage fermented with the novel probiotic L. lactis KX881782 could be a promising functional food that relatively provides several health benefits to consumers compared with fermented beef sausage.