한국인 폐확산능 정상예측식의 임상적 유용성과 정확성

나승원 ( Seung Won Ra ),박태선 ( Tai Sun Park ),홍윤기 ( Yoon Ki Hong ),홍상범 ( Sang Bum Hong ),심태선 ( Tae Sun Shim ),임채만 ( Chae Man Lim ),이상도 ( Sang Do Lee ),고윤석 ( Youn Suck Koh ),김우성 ( Woo Sung Kim ),김동순 ( Dong 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.2

연구배경: 폐확산능을 해석하는 데 필요한 정상예측식으로는 한국인을 대상으로 하여 박 등이 개발한 식(박 식)이 있으나 아직 외국 정상예측식을 많이 사용하고 있다. 이에 국내에서 많이 사용하는 외국 정상예측식인 Burrows 식과 박 식의 임상적 유용성과 정확성을 비교하고자 하였다. 방법: 1. 임상적 유용성 연구; 2006년 7월부터 12월까지 6개월간 폐확산능검사를 시행한 환자 중 두 식을 각각 정상예측식으로 적용하였을 때 폐확산능 해석이 다른 276명(대상군 A)을 대상으로 하였다. 대상군 A에게 두 식을 각각 적용하였을 때 폐확산능 해석과 임상적 판단과의 일치도를 비교하여 임상적 유용성을 평가하였다. 2. 간질성폐질환 진단의 정확성 비교; 2001년부터 2006년까지 폐조직검사를 시행하여 확진된 간질성폐질환군과 서울아산병원에서 모집한 정상군을 대상으로 하여 정상예측식으로 두 식을 각각 적용하였을 때 폐확산능 해석의 정확도를 비교하였고, 두 식이 차이가 나는지 맥니머의 카이스퀘어 검정을 하였다. 결과: 1. 임상적 판단과의 일치도 비교; 276명을 임상정보를 토대로 폐확산능을 예측하여 분류한 결과 정상 54명, 감소 220명, 불분명이 2명이었다. 예측식으로 박 식과 Burrows 식을 적용하였을 때 임상적 판단과 일치하는 환자는 각각 78%와 22%이었다(p<0.001). 2. 간질성폐질환 진단의 정확성 비교; 박 식은 민감도 90.1%, 특이도 100%이었고 Burrows 식은 민감도 64.2%, 특이도 100%로 민감도가 통계학적으로 유의하게 박 식이 높았다(p<0.001). 결론: 우리나라 정상예측식인 박 식이 외국 정상예측식인 Burrows 식을 정상예측식으로 적용하는 것보다 임상적 유용성이나 간질성폐질환 진단의 민감도에서 더 우월하였다. 향후 폐확산능검사의 정상예측식으로 박 식을 사용해야 할 것으로 사료된다. Background: Park et al. developed the Korean reference equation for the measurement of diffusing capacity in 1985. However, the equation has not been widely used in Korea and foreign reference equations have been popularly used. We intended to compare the clinical usefulness and the accuracy of the the Korean reference equation (Park`s equation) with that of the foreign equation (Burrows` equation) that is commonly used in Korea. Methods: 1. Evaluation of clinical usefulness; Among 1,584 patients who underwent diffusing capacity (DLCO) at the Asan Medical Center from July to December 2006, group A subjects included 276 patients who had different interpretations of DLCO in trials employing Burrows` equation and Park`s equation. Clinical assessment was decided by consensus of two respiratory physicians. In order to evaluate the clinical usefulness of Burrows` equation and Park`s equation, agreement of clinical assessment and DLCO interpretation were measured. 2. Evaluation of accuracy; Group B subjects were 81 patients with interstitial lung disease (ILD) and 39 normal subjects. The 81 ILD patients were diagnosed following a surgical lung biopsy. The accuracy of diagnosing ILD as well as sensitivity and specificity were evaluated according to the use of the reference equations (Burrows` equation and Park`s equation) for DLCO. Results: Agreement between clinical assessment and interpretation of DLCO was 22% for the use of Burrows` equation and 78% for the use of Park`s equation. The sensitivity and specificity of the Burrows` equation for diagnosing ILD were 64.2% and 100%. The sensitivity and specificity of the Park`s equation for diagnosing ILD were 90.1% and 100%. The sensitivity of the Park`s equation for diagnosing ILD was significantly higher than that of Burrows` equation (p<0.001). Conclusion: The Korean reference equation (Park`s equation) was more clinically useful and had higher sensitivity for diagnosing ILD than the foreign reference equation (Burrows` equation). (Tuberc Respir Dis 2008;64:80-86)

저칼슘식이로 사육한 생쥐의 골수세포 배양에 의한 파골세포양세포의 형성

박주철,강선주,임도선,김현만,고재승 대한구강해부학회 1995 대한구강해부학회지 Vol.19 No.1

Although it is now well established that osteoclasts are derived from mononuclear hematopoietic stem cells, most likely within the granulocyte-macrophage lineage, the exact differentiation process of osteoclast precusors has only been partially discovered. This study was carried out to investigate the following topics by examining the osteoclast-like cell formation from the calcium-deficient mouse bone marrow cells; 1) the effect of calcium deficiency on the formation of the mononuclear phagocytes and the mononuclear precusors of the osteoclast in the mouse bone marrow; 2) the effect of TNF (tumor necrosis factor)-α on the differentiation of osteoclast-like cells from the calcium-deficient mouse bone marrow culture; 3) the formation of the osteoclast-like cell from the calcium-deficient mouse bone marrow mononuclear cells and the relationship between the osteoblast cell line(MC3T3E1) and osteoclast-like cell formation; 4) formation of the osteoclast-like cells from the mononuclear cells which were col1ected from PTH-pretreated mouse bone marrow cells to investigate the relationship between PTH and calcium deficiency in osteoclast-like cell formation. Bone marrow cells were isolated from marrow of mouse feeding normal diet or calcium deficient diet for 7 and 14 days. Bone marrow mononuclear cells were fractionated by cetrifugation on Hypaque-Ficoli density gradients. PTH-pretreated mouse bone marrow mononuclear cells were obtained by collecting the nonadherent cells after the culture of normal mouse marrow cells with PTH for 6 days. The prepared cells were cultured on dentin disc at the concentration of 1.5-2 X 10^(6) cells per 0.5ml of a-MEM containing 10% FCS for 3, 5 and 7 days. Thereafter, TRAP staining, NSE staining, effect of calcitonin on the osteoclast-like cell and scanning electron microscope analysis of resorption lacunae on the dentin disc were performed. The results were as follows; 1. Number of the osteoclast precusors and mononuclear phagocytes in bone marrow was increased by calcium-deficient diet. 2. More osteoclast-like cells were formed from the calcium-deficient mouse bone marrow cells than from normal cells. 3. Formation of osteoclast-like cells was significantly stimulated by TNF-α in normal mouse bone marrow cells and calcium-deficient mouse bone marrow cells at concentration of l0ng/ml and 100ng/ml. 4. Osteoblast cell line(MC3T3E1) did not play an important role in osteoclast-like cell formation. 5. Formation of the osteoclast-like cells from the mononuclear cells which were collected from PTH-pretreated mouse bone marrow cells was similar to that of calcium-deficient mouse bone marrow mononuclear cells, which suggests that the effect of calcium deficiency in osteoclast formation may be mediated by PTH.

거주 형태에 따른 에너지 섭취량과 소비량의 균형도 조사

박순목,고성경,남인수,윤재만,임승현,전중기,이종삼 대구대학교 인문과학연구소 2008 人文科學硏究 Vol.31 No.-

We investigated that the effects of residential type on energy balance in college students. Total sixteen college students were participated in this study, all subjects were assigned one of three groups: either school attendee students group, self-governed living students group, dormitory students group. Routine physical activity level (for 5 days including three weekday and two weekend) and food intake were surveyed. For investigation of degree of physical activity, all subjects were requested to record on their physical movements as possible as detail should be obtained. To all subjects, five-day dietary log form was given, and used for examining of calorie intake from their routine diet. There was no statistical difference in energy intake and consumption in each. However energy consumption was significantly higher than energy uptake in school attendee students group. All other groups were shown a similar energy values between energy uptake and consumption. There were no significant differences in energy intake and consumption in any of experimental groups when comparisons were made between weekdays and weekend. As far as energy uptake was concerned it was no statistical difference in any of major nutrients among groups. In conclusion, partial imbalance was found between energy intake and uptake in school attendee groups. This may be due to their more active life style than other groups'. In future studies, better controlled study should be performed not only more subjects are recruited but also minor nutrients are included for examining of energy balance.

칼라영상시스템에 의한 색재현 연구

朴勝玉,朴正萬,金洪錫 대진대학교 1993 大眞論叢 Vol.1 No.-

The tristimulus values of an object color are dependent on both the spectral power distribution of the illuminant and the surface reflectance of the object. Furthermore the physical factors that are relevant to color image processing include camera sensor spectral response and monitor phosphor power distributions. In this paper, we measured the chromaticity coordinates of monitor reproducing colors and compared them to actual color chips. Also the similar comparisons are made under changing illuminant conditions.

칼라영상시스템의 칼라보정 연구

朴勝玉,朴正萬,金洪錫,辛鉉德,林在權 대진대학교 1994 大眞論叢 Vol.2 No.-

We proposed a color calibration algorithm by considering spectral characteristic of the chosen color reproduction system which can be applied to device independent using characteristic vectors of surface reflectance. To evaluate color reproducing characteristics we applied the proposed color calibration algorithm by choosing the color of Macbeth ColorChecker's twenty four as a test color set.

칼라영상시스템에 의한 물체색 측정연구

朴勝玉,朴正萬,金洪錫,林在權 대진대학교 1993 大眞論叢 Vol.1 No.-

To simulate accurate spectral reflectance from the red-green-blue output of a video digitizing system, a set of basis functions were computed. 1565 reflectance spectra of the chips in the Munsell Book of Color(Glossy) were measured with a spectrophotometer in KRISS. The autocorrelation matrix of these samples was formed and the eigenvectors and the eigenvalues of this matrix were computed by using Karhunen-Loeve transformation.

INCOTERMS 2000D의 限界와 INCOTERMS 1990과의 比較硏究

박남규,신승만 건국대학교 사회정책연구소 2001 사회과학연구 Vol.14 No.1

동일한 국제예후지표(International Prognostic Index)를 나타내는 미만성 대형 B-세포 림프종에서 Bcl-2와 p16의 임상적 중요성

박상은,박수진,곽승근,박남숙,천재민,윤환중,조덕연,김삼용,김진만 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

In Korea, malignant lymphoma is a common cancer, comprising about 2.7% of all malignant neoplasm. Diffuse large B cell lymphoma is the most common lymphoma, representing about 50% of all Non-Hodgkin's lymphoma. Diffuse large B-cell lymphoma is usually considered as heterogeneous group of neoplasms rather than a single clinicopathological entity. Clinical prognostic systems, including the International Prognostic Index (IPI), although useful to assess overall prognosis, embrace patients with heterogeneous prognoses. But International Prognostic Index scoring system is not sufficiently predict the prognosis. It is likely that the prognostic assessment of patients with diffuse large B-cell lymphomamight be improved by using biological features. Bcl-2 protein and p16 protein expression is recognized as useful biologic markers predicting the prognosis of patients with diffuse large B-cell lymphoma. To determine the clinical significance and prognostic value of bcl-2 and p16 proteins expression patterns, we studied 18 patients with de novo DLBL, whose archival pathology specimen were available for immunohistochemistry studies, atChungnam National University Hospital from September 1992 to December 2000. Archival specimens from each patient were immunostained with respective antibodies for bcl-2, p16. The results are as follows; 1) The median age was 54(rage : 37-69). There were 12 male patients(66.7%) and 6 female patients(33.3%) The 'B' symptom was abscentin all patients. The stages were as follows : Ⅰ, 2 patient(11.1%), Ⅱ, 10 patient(55.6%), Ⅲ, 4patient(22.2%) and Ⅳ, 2patient(11.1%). 3 patients(16.8%) had the elevated LDH level, 14 patients(77.8%) had the normal LDH level and 1 patients(5.6%) was not identified the LDH level. 2 patients(11.1%) had the bulky disease and 16 patients(88.7%) had no bulky diease. The distribution of ECOG status were O, 2 patients(11.1%c), 1, 14patients(77.8%) and 2, 2patients(11.1%). 2) Theimmunohistochemistry results are as follows bcl-2:+,10 patients(55.6%), bcl-2:-, 8patinets(44.4%), p16:+,3 patients(16.7%), p16:-, 15patients(83.3%) 3) After a median follow UP durations of 67 months, the median survival time was 57 months with a rage of 7-100+ months. 5-years overall survival rates was 44% by Kaplan-Meier method. 4) Reduced overall survival was demonstrated in the patients who expressed bcl-2 protein(P=0.0174). 5-year overall survivial rate was 12%(bcl-2 expression) versus 88%(no bcl-2 expression) 5) Among diffuse large B-cell lymphoma patients with IPI score 0-1, reduced overall survival was demonstrated with bcl-2 expression(P=0.023). 5-year overall survival rate was 18%(bcl-2 expression group) versus 100%(no bcl-2 expressiongroup) 6) Median survival durtation of diffuse large B-cell lymphoma patients negative for p16 expression was 57 months whereas p16 postive patients' median survival duration was not reached(P=0.4478). In diffuse large B-cell lymphoma patients with identical IPI scores, bcl-2 expression had additional prognostic value.

칼라모니터의 색특성 평가

朴勝玉,朴正萬,金洪錫 대진대학교 1993 大眞論叢 Vol.1 No.-

To Evaluate the colorimetric properties of color monitor, a set of assumptions about the monitor's performance are described. These are the assumption of phosphor constancy, phosphor independece, spatial uniformity, and temporal stability. The results of measurements that test the validity of these assumptions for a multisync color monitor are presented.

Carbofuran 土壤混入處理의 水稻害蟲 防除效果 및 水稻生育에 미치는 影響

朴亨萬,朴重秀,崔承允,李炯來 서울大學校 農科大學 1981 서울대농학연구지 Vol.6 No.1

In 1980, a field experiment was conducted to evaluate the effects of soil-incorporated Carbofuran (Furadan 3G) on some rice insect pests and on the growth of rice plants of the variety Milyang-23, which is one of the Korean leading varieties. The effects were compared with those of the conventional broadcast of Carbofuran and Diazinon as local standard method. The results obtained were as follows: (1) The soil incorporation of Carbofuran dramatically suppressed the plant damages of the rice stem maggots (Chlorops oryzae and Hyderellia sasakii) and thrips (Baliothips biformis) at tillering stage of the rice; plant damages observed were 0.5%(1.2 a.i. kg/ha) and 0.6%(1.8 a.i. kg/ha) for the rice stem maggots, and 1.3%(1.2 a.i. kg/ha) and no damage(1.8 a.i. kg/ha) for thrips while with broadcast of Carbofuran there were 65.9%(1.2 a.i. kg/ha) and 42.4%(1.8% a.i. kg/ha) for ricestem maggots, and for the thrips, 60.6%, 36.0% with Carvofuran baoadcast, and 83.5%, 90.7% with Diazinon treatment, and 97.8% for the untreated check.(2) Soil incorporation and broadcast of Carbofuran were significantly effective for the control of brown planthoppers (Nilaparvata lugens) and the white-backed planthoppers (Sogatella furcifera), and the conventional broadcast of Diazinon was still unsatisfactory for controlling the brown planthoppers. (3) on 38th day after transplanting, the number of tillers per hill and plant height in the soil incoporation of Carboruran revealed the significant increases over those of the Carbofuran and Diazinon brodcasts and the check; with number of tillers per hill 23.0 for 1.2 a.i. kg/ha and 25.7 for 1.8 a.i. kg/ha with number of tillers per hill 23.0 for 1.2 a.i. kg/ha and 25.7 for 1.8 a.i. kg/ha with Carbofuran soil incorporation, while they were for 20.1, 22.1, with respective doses of the broadcasted Carbofuran they were 19.1 for the broadcased Diazinon, and 18.5 for the check, on the 82nd day after transplanting, root development of rice plants grown on the Carbofuran soil incorporated sites also revealed a significant increase over the broadcasting treatments and the check in the number and length of roots.(4) Even under the unusual low-temperature conditions during 1980 in Korea, Carvofuran soil incorporation greatly promoted the heading of rice plants and consequently resulted in significant shortening of the heading time as compared to those of Carbofuran and Diazinon broadasts and the check. In addition, soil incorporation of Cabofuran resulted in much better heading status(65 to 70% in completed heading) than broadcast(35 to 37% in Carbofuran broadcast and 20% in Diazinon broadcast) and the untereated check(30%). (5) The mexmium growth promotion of rice plants was observed from the rate of 1.8 a.i. kg/ha of the Carbofuran soil incorporation in plant height (90.0cm), stem height(78.6cm), length of uppermost internodes(24.7cm), and root weight(5.0g/hill in dry base) over the untreated check(81.9cm in plant height, 62.9cm in stem height, 19.3cm in uppermost intermodes and 3.6g in root weight) and other insecticide treated plots. However, there was no singnificant difference in panicle length among the treatments. (6) The number of panicles per hill, number of grains per panicle and 1000-grain weight showed that Carbofural soil incorporation significantly increased those yield components as compared to the broadcast and the untreated check. Of the treatments, Carbofuran soin incorporation registered significantly higher grain yields (2,460kg/ha from 1.2 a.i. kg/ha and 2,244kg/ha from 1.8 a.i. kg/ha treatments) than Carbofuran broadcest (1,005kg/ha from 1.2 a.i. kg/ha and 1,185kg/ha from 1.8 a.i. kg/ha treatments), Diazinon broadcast(63kg/ha), and the check(849kg/ha). Showing the low yield potential of the test variety seemed to be cold-damage from unusual weather conditions in 1980. (7) Analysis of essential macro-and micro-element contents in rice plants on the 122nd day after transplanting showed more k, Cu, Mn and Zn contents in the plants treated by soil incorporation of Carbofuran than those in the untreated check, while N and Fe were lower in the plants treated by Carbofuran than those in the untreated check, while N and Fe were lower in the plants treated by Carbofuran soil incorporation. Amounts of K and Zn were much greater in Carbofuran soil incorporation than in Carbofuran broadcast, and N and P were greatly lower in the plants by Carbofuran soil incorporation. (8) in conclusion, the result obtained in this study would provide some real evidences that Carbofuran incoporated with the paddy soils could be a new insecticidal application method of the maximization of rice yield through the dramatical suppressin of the plant damages of insect pests; the rice leaf stem maggots, thrips, and planthoppers, and great growth stimulation(increasing the root development, the effective tillers, panicles, etc.) of rice plants.