http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Schmoller, Shelly K. (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
-
-
Wu, Chia-wei,Schmoller, Shelly K.,Shin, Sung Jae,Talaat, Adel M. American Society for Microbiology 2007 Journal of Bacteriology Vol.189 No.21
<B>ABSTRACT</B><P><I>Mycobacterium avium</I> subsp. <I>paratuberculosis</I> causes an enteric infection in cattle, with a great impact on the dairy industry in the United States and worldwide. Characterizing the gene expression profile of <I>M. avium</I> subsp. <I>paratuberculosis</I> exposed to different stress conditions, or shed in cow feces, could improve our understanding of the pathogenesis of <I>M. avium</I> subsp. <I>paratuberculosis</I>. In this report, the stress response of <I>M. avium</I> subsp. <I>paratuberculosis</I> on a genome-wide level (stressome) was defined for the first time using DNA microarrays. Expression data analysis revealed unique gene groups of <I>M. avium</I> subsp. <I>paratuberculosis</I> that were regulated under in vitro stressors while additional groups were regulated in the cow samples. Interestingly, acidic pH induced the regulation of a large number of genes (<I>n</I> = 597), suggesting the high sensitivity of <I>M. avium</I> subsp. <I>paratuberculosis</I> to acidic environments. Generally, responses to heat shock, acidity, and oxidative stress were similar in <I>M. avium</I> subsp. <I>paratuberculosis</I> and <I>Mycobacterium tuberculosis</I>, suggesting common pathways for mycobacterial defense against stressors. Several sigma factors (e.g., <I>sigH</I> and <I>sigE</I>) were differentially coregulated with a large number of genes depending on the type of each stressor. Subsequently, we analyzed the virulence of six <I>M. avium</I> subsp. <I>paratuberculosis</I> mutants with inactivation of differentially regulated genes using a murine model of paratuberculosis. Both bacterial and histopathological examinations indicated the attenuation of all gene mutants, especially those selected based on their expression in the cow samples (e.g., <I>lipN</I>). Overall, the employed approach profiled mycobacterial genetic networks triggered by variable stressors and identified a novel set of putative virulence genes. A similar approach could be applied to analyze other intracellular pathogens.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
