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      저자 : Sana Sheik (검색결과 2 건)
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        Endophytic fungi associated with Psychotria flavida Talbot., an endemic plant of Western Ghats (India) and their bioactive potential

        Sana Sheik,K. R. Chandrashekar,Sukesh Shirlal 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.2

        Psychotria flavida Talbot. an endemic plant of Western Ghats was explored for endophytic mycoflora. The endophytic fungi isolated from leaf and stem were identified with the aid of morphology and molecular methods. Lasiodiplodia theobromae and Picchia guillermondii were commonly present in both the sites. Overall colonization frequency of leaf samples was found to be higher than stem samples in both the places. Studies on seasonal variation of the occurrence of these fungi indicated a higher frequency during winter as compared to monsoon and summer. Further, endophytic fungi were verified for bioactive diagnosis using in vitro antimicrobial (disc diffusion method) and antioxidant (DPPH free radical scavenging). Among the 15 crude extracts of different endophytes tested, the extracts of Lasiodiplodia theobromae possessed potentially better antibacterial and antioxidant activity. The present study divulges that the endophytic fungi L. theobromae could be a potential source of antimicrobial and antioxidant drugs.

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        Fungal endophytes of an endemic plant Humboldtia brunonis wall. of western Ghats (India) and their antimicrobial and DPPH radical scavenging potentiality

        Sana Sheik,K. R. Chandrashekar 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.2

        Endophytic fungi associated with leaf and stem samples of Humboldtia brunonis Wall, a dominant myrmecophyte, commonly known as ‘hasige mara’, endemic to the South Western Ghats of India were enumerated from two sites viz. Charmadi and Mangalore University arboretum and tested for antimicrobial activity. Aspergillus sp., Curvularia clavata, Curvularia pallescens, Debaromyces hansenii, Guignardia sp., Hypoxylon anthochroum, Meyerozyma caribbica, Paecilomyces lilacinus were found in leaf samples of H. brunonis. Alternaria alternata, Cunninghamella echinulata, Fusarium fusarioides, Fusarium oxysporum, Phanerochaete sp. were found in stem samples. Lasiodiplodia theobromae was commonly present in both the samples. The zone of inhibition by the endophytic fungi Curvularia pallescens isolated from H. brunonis for Candida albicans was up to 24 mm diameter, which is comparable to the inhibition by the antifungal standard Nystatin (24 mm). In seven endophytic fungi possessed free radical scavenging activity at 10 μg/ml, the highest being in C. pallescens extracts. In the present study, C. pallescens exhibited better antimicrobial and antioxidant activity compared to the other extracts. The presence of mycotoxin curvularin was reported in the partially purified fraction of C. pallescens.

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