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RISS 인기검색어
- 검색키워드
- 저자 : S.S. LEE(李洙聖) (검색결과 2 건)
- 무료
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여름무우 品種改良에 있어서 單株系統法의 利用 硏究(1) F₁品種의 兩親系統 育成法으로서의 可能性 檢討
S.S. Lee(李洙聖),W. M. Yoon(尹禾模) 한국육종학회 1984 한국육종학회지 Vol.16 No.2
The single seed descent method was tried for developing parental inbreds of an F₁ hybrid variety for summer production of radish. A double cross population was provided by crossing a virus resistant plant of the variety Minowase to the commercial true variety Yonghyeon which was mild-resistant, and then to the commercial F₁ variety Taebaeck which was very susceptible to virus disease. Fifteen percent out of 185 inbreds at the F₅ generation was resistant or tolerant to virus and soft rot diseases. At least three different self-incompatibility alleles were identified to be involved in the 5 lines selected finally and S-allele segregation was detected in three inbreds out of these selections. Heterosis of F₁ hybrids between selections seemed to be enough to meet the breeding goal. Consequently, it is considered that the single seed descent technique can be applied to developing inbred lines for an F₁ hybrid suitable for summer production of radish, which is an out-crossing crop, on low land.
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An In vitro pollination technique for detecting self-incompatibility in cruciferous crops
S.S. LEE(李洙聖),J.Y. YOON(尹震映),H.M. YOON(尹禾模) 한국육종학회 1982 한국육종학회지 Vol.14 No.3
An in vitro pollination procedure was developed as an efficient technique for assaying self-incompatibility of Cruciferous crops. Excised flowers were pollinated in the lab, kept in constant relative humidity chambers at 25℃, and the pollen tubes in the style were counted using fluorescence microscopy after staining with aniline blue the next day. A relative humidity (RH) of 98% was optimum for differentiating self-incompatibility vs. self-compatibility with excellent reproducibility. The number of pollen tubes penetrating to stigma under 98% RH was different from plant to plant and age to age of the flower. However. this variation for flowers of the same plant was highly correlated with seed set. Strongly self-incompatible plants could be selected by selecting for the minimum number of pollen tubes across a wide range of flower age for individual plants. This technique seemed to be widely applicable in the study of self-incompatibility.
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