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Rongli Mo,Na Zhang,Sichao Yang,Qinglin Zhang,Zhengrong Luo 한국원예학회 2016 원예과학기술지 Vol.34 No.2
A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5’ and pHG-PDS3’) targeted DkPDS, which also led to significantly reduce expression of DkPDS in ‘Mopanshi’ persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in ‘Mopanshi’ and ‘Eshi 1’ leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in ‘Mopanshi’, ‘Eshi 1’ and ‘Youhou’ leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.
Mo, Rongli,Zhang, Na,Yang, Sichao,Zhang, Qinglin,Luo, Zhengrong Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.2
A transient ihpRNA-induced gene silencing system based on Agrobacterium-mediated injection infiltration has been established to evaluate candidate genes involved in proanthocyanidin (PAs) biosynthesis in persimmon (Diospyros kaki Thunb.). We chose DkPDS (phytoene desaturase) as a gene-silencing target to evaluate the newly developed transient gene silencing system. Our qRT-PCR analysis indicated that two ihpRNA constructs (pHG-PDS5' and pHG-PDS3') targeted DkPDS, which also led to significantly reduce expression of DkPDS in 'Mopanshi' persimmon leaves. To further confirm the reliability of the system, we successfully utilized it for DkLAR (leucoanthocyanidin reductase) gene silencing. The expression levels of DkLAR in 'Mopanshi' and 'Eshi 1' leaves were ca. 6-fold and ca. 5-fold lower than those in leaves harboring empty vector (pHG-GFP), respectively. DMACA (4-dimethylaminocinnamaldehyde) staining and the Folin-Ciocalteau assay showed that the accumulation of PAs was markedly inhibited in 'Mopanshi', 'Eshi 1' and 'Youhou' leaves. These results indicate that DkLAR plays an important role in the accumulation of PAs in persimmon. The transient ihpRNA-induced gene silencing method developed in this study is a highly efficient and useful tool for functional analysis of persimmon genes involved in PA biosynthesis.
( Sheng Rong Liu ),( Qing Ping Wu ),( Ju Mei Zhang ),( Shu Ping Mo ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3
ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.
Dong-Ming Mo,Liu-Zhu Cao,Li Zhang,Shuang Ye,You-Rong Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10
We report a set of direct numerical simulation results on Rayleigh-BénardMarangoni (R-B-M) flow of cold water in the cylindrical pools. The heat transfer between the free surface and the environment is considered. The aspect ratio Г of the cylindrical pool varies from 2 to 8. Rayleigh (Ra) and Biot (Bi) numbers are respectively confined in Ra ≤ 10 4 and 0 < Bi ≤ 50. The flow onset critical Ra is determined and the influences of Bi and the density inversion parameter ( mΘ ) on the critical Ra are analyzed. The primary bifurcation flow structures of R-B-M flow are shown and the evolution of the flow structures with Ra and Bi at different mΘ is observed. Furthermore, the heat transfer ability is estimated by Nusselt number. The results indicate that the critical Ra of the flow onset increases with increasing Bi and Θ m . But it decreases with the increase of Г. The primary bifurcation pattern is multicellular flow. With increasing Г, the number of flow cells in multicellular flow increases fast. With increasing Ra, the up-triangular and up-quadrilateral flow structures appear at Г = 4, and finally transits to the up one-torus. With increasing Ra and Г, and decreasing Θ m , average Nusselt number increases monotonically. However, with increasing Bi, it first increases, and then decreases.
( Liu Sheng Rong ),( Qing Ping Wu ),( Ju Mei Zhang ),( Shu Ping Mo ),( Xiao Juan Yang ),( Chun Xiao ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
ε-Poly-L-lysine (ε-PL), produced by Streptomyces or Kitasatospora strains, is a homo-poly-amino acid of Llysine, which is used as a safe food preservative. The present study investigates the combined use of cell immobilization and in situ adsorption (ISA) to produce ε-PL in shaken flasks. Loofah sponge-immobilized Streptomyces ahygroscopicus GIM8 produced slightly more ε-PL than those immobilized on synthetic sponge, and sugarcane bagasse. Moreover, loofah sponge supported the maximum biomass. Hence, loofah sponge was chosen for cell immobilization. Meanwhile, the ion-exchange resin D152 was employed for ISA. The loofah sponge-immobilized cells produced 0.54 ± 0.1 g/l ε-PL, which significantly increased to 3.64 ± 0.32 g/l after combining with ISA through the addition of resin bags. The free cells with ISA using the dispersed resin yielded 2.73 ± 0.26 g/l of ε-PL, an increase from 0.82 ± 0.08 g/l. These data illustrate that the proposed combination method improved production most significantly compared with either immobilization or ISA only. Moreover, the immobilized cells could be repeatedly used and an ε-PL total amount of 8.05 ± 0.84 g/l was obtained. The proposed combination method offers promising perspectives for ε-PL production.
He, Jian-Rong,Xi, Jing,Ren, Ze-Fang,Qin, Han,Zhang, Ying,Zeng, Yi-Xin,Mo, Hao-Yuan,Jia, Wei-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
Purpose: Complement receptor 1 (CR1) is induced by Epstein-Barr virus (EBV) and may be a potential biomarker of nasopharyngeal carcinoma (NPC). We conducted the present study to evaluate the association of CR1 expression with clinicopathological features and prognosis of NPC. Methods: We enrolled 145 NPC patients and 110 controls. Expression levels of CR1 in peripheral blood mononuclear cells (PBMCs) were detected using quantitative real-time PCR and associations with clinicopathological features and prognosis were examined. Results: CR1 levels in the NPC group [3.54 (3.34, 3.79)] were slightly higher than those in the controls [3.33 (3.20, 3.47)] (P<0.001). Increased CR1 expression was associated with histology classification (type III vs. type II, P=0.002), advanced clinical stage (P=0.003), high T stage (P=0.017), and poor overall survival (HR, 4.89; 95% CI, 1.23-19.42; P=0.024). However, there were no statistically significant differences in CR1 expression among N or M stages. Conclusion: These findings indicate that CR1 expression in PBMCs may be a new biomarker for prognosis of NPC and a potential therapeutic target.
Shengquan Li,Rong Zhao,Yueping Mo,Zhenyu Sun,Juan Li 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.3
This paper presents a composite control strategy for the active suppression of vibration due to the unknown disturbances, such as external excitation, harmonic effects and control spillover, as well as high-frequency accelerometer measurement noise in the all-clamped stiffened plate. The proposed composite control action based on the modal approach, consists of two contributions including feedback part and feedforward part. The feedback part is the well-known PID controller, which is widely used to increase the structure damping and improve its dynamic performance close to the resonance frequencies. In order to get better performance for vibration suppression, the weight matrixes is optimized by chaos sequence. Then an improved disturbance observer (IDOB) as the feedforward compensation part is developed to enhance the vibration suppression performance of PID under various disturbances and uncertainties. The proposed IDOB can simultaneously estimate the various disturbances dynamically as well as measurement noise acting on the system and suppress them by feedforward compensation design. A rigorous analysis is also given to show why the IDOB can effectively suppress the unknown disturbances and measurement noise. In order to verify the proposed composite control algorithm (IDOB-PID), the dSPACE real-time simulation platform is used and an experimental platform for the all-clamped stiffened plate active vibration control system is set up. The experimental results demonstrate the effectiveness, practicality and strong anti-disturbances ability of the proposed control strategy.
Yu Cui,Li Yong,Mo Rongli,Deng Wen,Zhu Zhixian,Hu Xingming 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.6
The ecological distribution and activities of soil microbes can refl ect the infl uence of environmental factors on microbes andchanges in soil fertility. Understanding the community structure of the soil microbes is of great importance to maintaining soilhealth in ancient mulberry plantation ecosystems. In this study, the population structure of soil microorganisms in diff erentages of mulberry plantations was analyzed using high-throughput sequencing technology. A total of 1616, 1568, 1379 and1477 operational taxonomic units were acquired from samples of 3Y (3-year-old), 35Y (35-year-old), 200Y (200-year-old)and 600Y (600-year-old) soils, respectively. The results exhibited that the highest community diversity was found in 35Ysoil, followed by 200Y soil, and showed that the stand age strongly infl uenced the community structure of soil bacteria. Atotal of 23 phyla were detected in 12 samples, and the genus of bacteria that predominated the diff erent stand ages mainlyincluded; Phenylobacterium , Cohnella , Lactococcus , Sphingomonas , Bacillus , and Microbispora . The relative abundanceof Phenylobacterium accounts for 22.6–53.5% of the total abundance. This study showed that the richness and diversity ofthe soil bacteria decreased as the stand age increased in ancient mulberry plantations.