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Overexpression of a maize MYB48 gene confers drought tolerance in transgenic arabidopsis plants
Yan Wang,Qianqian Wang,MingLi Liu,Chen Bo, Xi Wang,Qing Ma,Beijiu Cheng,Ronghao Cai 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
MYBs are a vital family of transcription factorsthat play critical roles in plant development and stressresponse. However, knowledge concerning the functions ofMYBs in the non-model plants is still limited. In this study,we isolated a R1-type MYB gene from maize (Zea mays L.),designated as ZmMYB48. Quantitative RT-PCR analysisdemonstrated that ZmMYB48 expression was induced bydrought and ABA treatments. Subcellular localization analysisrevealed that ZmMYB48 protein was targeted to the nucleusin tobacco leaf epidermal cells. Transactivation assay in yeastdemonstrated that ZmMYB48 had transcriptional activationability. Heterologous overexpression of ZmMYB48 inArabidopsis remarkably improved plant tolerance to droughtstress, as determined through physiological analyses ofsurvival rate, relative water content, malonaldehyde content,relative electrolyte leakage and proline content. Moreover,overexpression of ZmMYB48 enhanced the expression ofstress/ABA-responsive genes such as P5CS1, RD22, RD29Band ABI1. In addition, ZmMYB48-overexpressing plantsaccumulated higher content of ABA than WT plants underdrought stress. These results demonstrate that ZmMYB48might act as a positive regulator that participates in thedrought stress response through ABA signalling.