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RISS 인기검색어
- 검색키워드
- 저자 : Rabindra N. Padhy (검색결과 3 건)
- 무료
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Debasmita Dubey,Rajashree Patnaik,Goutam Ghosh,Rabindra N. Padhy 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.5
Objectives: To locate a plant with suitable phytochemicals for use as antimicrobial agents to control multidrug-resistant (MDR) bacteria as a complementary medicine, without host toxicity as monitored through cultured lymphocytes from human umbilical cord blood. Methods: The methanol crude leaf extract of the plant Woodfordia fruticosa was subjected to antimicrobial assay in vitro with nine pathogenic MDR bacteria from clinical samples. This was followed by bioassay-guided fractionation with seven non-polar to polar solvents, gas chromatographyemass spectrometry analysis of the n-butanol fraction, and monitoring of the host toxicity of the leaf extract with in vitro grown lymphocytes from human umbilical cord blood. Results: The leaf extract of W. fruticosa had a controlling capacity for MDR bacteria. The minimum inhibitory concentration and minimum bactericidal concentration of the n-butanol fraction were < 1.89 mg/mL extract and 9.63 mg/ mL extract, respectively. The gas chromatographyemass spectrometry spectrum of the n-butanol fraction confirmed the presence of 13 peaks of different compounds with retention times of 9.11 minutes, 9.72 minutes, 10.13 minutes, 10.78 minutes, 12.37 minutes, 12.93 minutes, 18.16 minutes, 21.74 minutes, 21.84 minutes, 5.96 minutes, 12.93 minutes, 24.70 minutes, and 25.76 minutes. The six leading compounds were: diethyl phthalate: IUPAC name: diethyl benzene-1,2- dicarboxylate; 5-methyl-2-(1-methylethyl) phenol: IUPAC name: 5-methyl-2-propan-2-ylphenol; (E )-3,7-dimethylocta-2,6-diene-1-thiol: IUPAC name: (2Z)- 3,7-dimethylocta-2,6-diene-1-thiol; 2,6,10-dodecatrien-1-ol, 3,7,11-trimethyl-, (E,E ): IUPAC name: 2,6,10-dodecatrien-1-ol; 3,7,11-trimethyl-, (E,E); 2- methoxy-4-(2-propenyl) phenol: IUPAC name: 2-methoxy-4-[(1E)-prop-1-en-1-yl] phenol; hexadecanoic acid: IUPAC name: hexadecanoic acid. Conclusion: The presence of antimicrobial compounds that are therapeutically potent against MDR bacteria was confirmed in W. fruticosa. The crude leaf extract showed no host toxicity with human lymphocytes; the n-butanol fraction of the extract was the most suitable bioactive fraction. The terpenes isolated were: 5-methyl-2-(1-methylethyl) phenol, 2-methoxy-4-(2-propenyl) phenol, 2,6-octadien-1-ol, 3,7-dimethyl-(E )-2,6-octadienal, 3,7-dimethylcyclohexanol, and cyclohexanol, 2-methylene-5-(1-methylethenyl) which were reported to have specifically antimicrobial activity.
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Sibanarayan Rath,Rabindra N. Padhy 한국한의학연구원 2014 Integrative Medicine Research Vol.3 No.3
Background: To screen methanolic extracts of 26 commonly used Indian spices against ninespecies of uropathogenic bacteria (Enterococcus faecalis, Staphylococcus aureus, Acinetobacterbaumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Pro-teus mirabilis, and Pseudomonas aeruginosa), isolated from clinical samples of a tertiary carehospital for antibacterial activity.Methods: Bacterial strains were subjected to antibiotic sensitivity testing by Kirby–Bauer’sdisc diffusion method. Monitoring antibacterial potentiality of spice extracts was done bythe agar-well diffusion method with multidrug resistant (MDR) strains of nine uropathogens.Results: The Gram-positive (GP) bacteria E. faecalis and S. aureus were resistant to 16 of the21 antibiotics used. Among the Gram-negative (GN) bacteria, resistant patterns were A. bau-mannii and E. aerogenes to 12, C. freundii to 14, E. coli to 12, K. pneumoniae to 10, P. mirabilisto 11, and P. aeruginosa to 15 antibiotics of the 18 antibiotics used. The most effective 15spices, having at least 25–29 mm as the size of the zone of inhibition, were Allium cepa,Brassica juncea, Cinnamomum tamala, Cinnamomum zeylanicum, Coriandrum sativum, Cuminumcyminum, Curcuma longa, Mentha spicata, Murraya koenigii, Nigella sativa, Papaver somniferum,Piper nigrum, S. aromaticum, Trachyspermum ammi, and Trigonella foenum for at least one ofthe GP or GN MDR bacterial strains used. Moderate control capacity was registered by ninespices, Curcuma amada, Foeniculum vulgare, Illicium verum, Mentha spicata, Papaver somniferum,Syzygium aromaticum, Trachyspermum ammi, Trigonella foenum, and Zingiber officinale. However,the best two spices for controlling all the pathogens used were C. zeylanicum and C. longa,with the highest value of 29 mm as the inhibition zone size.Conclusion: The most effective and unique 16 spice plants recorded for the in vitro control ofMDR uropathogens could further be pursued for the development of complementary andsupplementary medicine against MDR bacteria.
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