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- 저자 : Pengcheng Jin (검색결과 5 건)
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Qing-Qing Li,JIN SHENG,Pengcheng Li,Dapeng Li,Cheng-Chao Zheng,Dequan Li,Huairui Shu 한국식물학회 2012 Journal of Plant Biology Vol.55 No.4
Alcohol acyltransferase (AAT) plays an important role in ester biogenesis in ripening fruit. In apple, MdAAT2 is up-regulated in response to treatment with defense-related hormones. We cloned a novel MdAAT2 promoter via thermal asymmetric interlaced PCR (TAIL-PCR). Sequence analysis indicated the presence of salicylic acid (SA)- and methyl jasmonate (MeJA)-responsive elements in the promoter region. To examine further the operational mechanism for transcription factors (TFs) in regulating expression of the MdAAT2 promoter, we isolated and investigated six potential stress-induced TFs -- MdMYB1, MdMYB2, MdMYB6,MdERF1, MdERF2, and WRKY. These were monitored during various stages of fruit development as well as under several hormonal treatments. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that all of these TF genes, as well as MdAAT2, were detectable in the stigmas. Levels of expression by MdMYB1, MdMYB2, and MdERF1were significantly correlated with that by MdAAT2 during fruit ontogeny. Moreover, transcription of three TFs (MdMYB1,MdMYB6, and MdERF1) was significantly correlated with that of MdAAT2 upon exposure to ethephon, SA, or MeJA. Sequence analysis demonstrated that CAAT, CCAAT elements,and several MYB transcription factor binding sites (CNGTTmotif,AACCA, and CTAACCA elements) existed in the MdAAT2 promoter region. Using that analysis, we performed modeling of MdMYB1 and MdMYB6 based on PROTEINDNA complex1 and complex3 (PDB Accession Numbers 1H88 and 1H8A). There, similarities were found with the protein complex of CAAT or CCAAT consensus sequences. Thus, we propose that two TFs -- MdMYB1 and MdMYB6-- are directly or indirectly involved in the activation of MdAAT2 expression in apple fruit.
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Le Qi,Jianxing Zhou,Pengcheng Jin,Mingming Wang,Yongzheng Su,Hengyu Zhou,Guochun Liu,Lirui Song 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9
In this paper, a rib layout optimization method that can efficiently reduce the radiated noise of gearboxes is proposed. Taking the secondary gearbox as the research object, the radiated noise of the housing is calculated using the FEM/BEM method, the process of determining the region with the maximum acoustic contribution is illustrated, and a topology optimization model that can reduce the air noise on the field points of the gearbox is established with the surface normal velocity of the region as the optimization target. The method is applied to add new noise reduction ribs on the surface of the gearbox. The results show that after combining the topology optimization with the acoustic contribution analysis, the sound pressure in the area with the highest sound pressure on the gearbox sound field is significantly reduced by adding new noise reduction ribs according to the optimization results.
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Tao, Longlong,Chen, Liwei,Long, Pengcheng,Chen, Chunhua,Wang, Jin Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.2
Current risk assessment of Spent Nuclear Fuel (SNF) transportation has the problem of the incomplete risk factors consideration and the general particle diffusion model utilization. In this paper, the accident frequency calculation and the detailed simulation of the accident consequences are coupled by the integrated risk assessment method. The "man-machine-environment" three-dimensional comprehensive risk indicator system is established and quantified to characterize the frequency of the transportation accidents. Consideration of vegetation, building and turbulence effect, the standard k-ε model is updated to simulate radioactive consequence of leakage accidents under complex terrain. The developed method is applied to assess the risk of the leakage accident in the scene of the typical domestic SNF Road Transportation (SNFRT). The critical risk factors and their impacts on the dispersion of the radionuclide are obtained.
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Zhu, Yingfang,Schluttenhoffer, Craig M.,Wang, Pengcheng,Fu, Fuyou,Thimmapuram, Jyothi,Zhu, Jian-Kang,Lee, Sang Yeol,Yun, Dae-Jin,Mengiste, Tesfaye American Society of Plant Biologists 2014 The Plant cell Vol.26 No.10
<P>This work explores the intriguing roles of Mediator subunit CYCLIN-DEPENDENT KINASE8 (CDK8) in plant immune responses to fungal infection. CDK8 regulates jasmonate-responsive gene expression and cuticle development via interactions with MEDIATOR COMPLEX SUBUNIT25 and transcription factor WIN1, respectively, while other interactions suggest evolutionary conservation of the Mediator kinase module.</P><P>CYCLIN-DEPENDENT KINASE8 (CDK8) is a widely studied component of eukaryotic Mediator complexes. However, the biological and molecular functions of plant CDK8 are not well understood. Here, we provide evidence for regulatory functions of <I>Arabidopsis thaliana</I> CDK8 in defense and demonstrate its functional and molecular interactions with other Mediator and non-Mediator subunits. The <I>cdk8</I> mutant exhibits enhanced resistance to <I>Botrytis cinerea</I> but susceptibility to <I>Alternaria brassicicola</I>. The contributions of CDK8 to the transcriptional activation of defensin gene <I>PDF1.2</I> and its interaction with MEDIATOR COMPLEX SUBUNIT25 (MED25) implicate CDK8 in jasmonate-mediated defense. Moreover, CDK8 associates with the promoter of <I>AGMATINE COUMAROYLTRANSFERASE</I> to promote its transcription and regulate the biosynthesis of the defense-active secondary metabolites hydroxycinnamic acid amides. CDK8 also interacts with the transcription factor WAX INDUCER1, implying its additional role in cuticle development. In addition, overlapping functions of CDK8 with MED12 and MED13 and interactions between CDK8 and C-type cyclins suggest the conserved configuration of the plant Mediator kinase module. In summary, while CDK8’s positive transcriptional regulation of target genes and its phosphorylation activities underpin its defense functions, the impaired defense responses in the mutant are masked by its altered cuticle, resulting in specific resistance to <I>B. cinerea</I>.</P>
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Le Qi,Quanwei Cui,Cheng Wang,Xueyan Sun,Janxing Zhou,Pengcheng Jin,Guochun Liu,Lirui Song 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12
In this paper, a simplified acoustic optimization method is proposed to replace the sound power with the maximum normal velocity of the surface as a direct target, a topological optimization model of the variable density method is established to reduce the acoustic radiation of the structure, and the relationship between the form of damping distribution and the acoustic radiation is investigated. The complex modulus model is used to describe the viscoelastic material intrinsic relationship of the damping layer. The effects of different excitation frequencies on the topology optimization results are discussed. The radiated noise is simulated using the finite element method and the boundary element method. Five plates are fabricated according to the damping layer layout for different single-frequency excitations. Modal and acoustic experiments are carried out to validate the proposed method. The numerical and experimental results show that there is a significant reduction in the sound pressure level (SPL).
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