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- 저자 : Pasitka, Laura (검색결과 1 건)
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A Microbore Tubing Based Spiral for Multistep Cell Fractionation
Pasitka, Laura,van Noort, Danny,Lim, Wanyoung,Park, Sungsu,Mandenius, Carl-Fredrik American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.21
<P>Cells were separated with the aid of a multistep spiral fractionation device, utilizing hydrodynamic forces in a spiral tubing. The spiral was fabricated using “off-the-shelf” microbore tubing, allowing for cheap and fast prototyping to achieve optimal cell separation. As a first step, a model system with 20 and 40 μm beads was used to demonstrate the effectiveness of the multistep separation device. With an initial purity of 5%, a separation purity of 83% was achieved after a two-step separation with the addition of 0.1% polyethylene glycol (PEG)-8000. Next, doxorubicin-resistant polyploid giant breast cancer cells (MDA-MB-231) were separated from doxorubicin-sensitive monoploid small breast cancer cells in the same fashion as the beads, resulting in a purity of around 40%, while maintaining a cell viability of more than 90%. Combined with basic cell analytical methods, the hydrodynamic separation principle of the device could be envisaged to be useful for a variety of cell fractionation needs in cell biology and in clinical applications.</P> [FIG OMISSION]</BR>
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