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        A novel hydrogel containing 4-methylcatechol for skin regeneration: in vitro and in vivo study

        Jilla Majidi Ghatar,Arian Ehterami,Simin Nazarnezhad,Maryam Sadat Hassani,Nariman Rezaei Kolarijani,Solmaz Mahami,Majid Salehi 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.3

        Skin damages are usual physical injuries and different studies have been done to improve wound healing. Hydrogel dueto its properties like a moist environment and cooling wound site is a good option for wound treatment. In this study, weevaluated the consequence of using alginate/chitosan hydrogel contained various dosages of 4-Methylcatechol (0, 0.1, 1%(W/W)) on wound healing. After hydrogel fabrication, different tests like SEM, swelling, release, weight loss, and hemoandcytocompatibility were done to characterize fabricated hydrogels. Finally, the rat model was used to assess Alginate/Chitosan hydrogel's therapeutic function containing 0.1 and 1% of 4-Methylcatechol. The pore size of hydrogel was between24.5 ± 9 and 62.1 ± 11.63 μm and about 90% of hydrogel was lost after 14 days in the weight loss test. Blood compatibilityand MTT assay showed that hydrogels were nontoxic and improved cell proliferation. In vivo test showed that Alginate/Chitosan/0.1%4-Methylcatechol improved wound healing and the results were significantly better than the gauze-treatedwound. Our results showed dose depending effect of 4-Methylcatechol on wound healing. This study shows the treatmenteffect of 4-Methylcatechol on wound healing and the possibility of using it for treating skin injuries.

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