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RISS 인기검색어
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- 저자 : Myeongeun Yun (검색결과 2 건)
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Denaturing gradient gel electrophoresis를 이용한 한국의 논 토양 미생물 다양성 분석 방법
최명은 ( Myeongeun Choe ),홍성준 ( Sung Jun Hong ),임종희 ( Jong Hui Lim ),곽윤영 ( Yun Young Kwak ),백창기 ( Chang Gi Back ),정희영 ( Hee Young Jung ),이인중 ( In Jung Lee ),신재호 ( Jae Ho Shin ) 한국응용생명화학회 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.2
Soil microbes are important integral components of soil ecosystem which have significant and diverse role in organic matter decomposition, nitrogen cycling, and nitrogen fixation. In this study an effective denaturing gradient gel electrophoresis (DGGE) method was employed for paddy soil microbial diversity survey. For optimum paddy soil microbial DNA extraction, different methods such as Lysis buffer, skim milk bead, sodium phosphate buffer, Epicentre Soil Master DNA extraction kit (Epicentre, USA) and Mo Bio Power Soil DNA kit (MO BIO, USA) methods were utilized. Among all the method, using Mo Bio Power Soil kit was most effective. DGGE analysis of Bacteria was carried out at 6% polyacylamide gel and 45-60% denaturing gradient in the optimal conditions. Whereas DGGE analysis of fungi was done at 6% polyacrylamide gel and 45-80% denaturing gradient in the optimal conditions. By applying the above assay, it was found that variation within the microbial community of paddy soil occurs by a factor of time. DGGE say used in this study through for a variety of soil microbial analysis suggests the potential use of this method.
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Comparison of Swallowing Characteristics in Normal Controls and Patients with Dysphagia
Baekhee Lee,Hyewon Lee,Myeongeun Yun,Mee Kyung Suh,Duk L. Na,Heecheon You 대한인간공학회 2013 대한인간공학회 학술대회논문집 Vol.2013 No.5
Objective: The present study is intended to compare swallowing characteristics between normal controls and patients with dysphagia through quantification of the pharyngeal movement. Background: The existing diagnoses of swallowing have been qualitatively conducted by a clinician referring to results of the VideoFluorocopic Swallowing Study (VFSS) or the Fiberoptic Endoscopic Evaluation of Swallowing (FEES); therefore, a quantitative methodology for assessment of the swallowing is required to diagnose dysphagia more accurately. Method: A three-step approach was applied in the study: (1) development of a swallowing measurement device consisting of an ultrasonic Doppler sensor to measure the pharyngeal movement, (2) establishment of five swallowing quantification measures (peak amplitude, duration, number of peaks, peak interval, and impulse of swallowing) by a swallowing signal preprocessing, (3) evaluation of the swallowing in 120 normal controls and 36 dysphagic patients by three-step protocol (S1. informed consent, S2. exercise, S3. swallowing session; type and volume: dry saliva, thin liquid 1, 3, 9 ml, and thick liquid 1, 3, 9 ml). Results: Swallowing signals of normal controls and dysphagic patients classified into 2 types (short-single: 39%; short-double: 43%) and 3 types (short-double: 58%; long-double: 33%; long-multiple: 9%), respectively. Dysphagic patients had difficulties in swallowing of 9 ml regardless of swallowing type. Highest peak amplitude, duration, number of peaks, average peak interval, and impulse of dysphagic patients were 1.3 times higher (t = 4.31, p < .001), 3 times longer (t = -11.15, p < .001), 2 times more (t = -6.73, p < .001), 2 times longer (t = -9.23, p < .001), and 1.3 times lower (t = 8.94, p < .001) than those of normal controls, respectively. Application: The swallowing characteristics of dysphagic patients can be applied to develop a diagnostic model which can evaluate quantitatively the existence and severity for dysphagia.
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