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Mohammad Reza Nasri Nasrabadi,Seyed Hadi Razavi 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4
In an attempt to accumulate lycopene in the bacterium Dietzia natronolimnaea HS-1 cultured in beet molasses, the influence of lycopene cyclase inhibitors on carotenogenesis (within the range 0-60 ppm) were evaluated. All inhibitors blocked the biosynthesis of canthaxanthin and resulted in accumulation of lycopene as the predominant carotenoid. As the final step of a study aiming at the optimization of culture conditions, a central composite design to a fed-batch process was applied to optimize the concentrations of lycopene cyclase inhibitors (within the range 0-50 ppm) in order to achieve high level accumulation of lycopene from D. natronolimnaea HS-1cultured in beet molasses. On the basis of this approach,the optimum concentrations of lycopene cyclase inhibitors required to achieve the highest level of lycopene accumulation (8.26±0.17 mg/L) were determined as follows: imidazole,24.74 ppm; nicotinic acid, 28 ppm; piperidine, 24.05 ppm;pyridine, 27.6 ppm; and triethylamine, 23.22 ppm.
Mohammad Reza Nasri Nasrabadi,Seyed Hadi Razavi 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2
The present work investigated on carotenogenesis with high β-carotene content by a new isolated highactivity strain-producer Rhodotorula acheniorum mutant MRN in cheese whey ultrafiltrate. After a serial of UV,ethymethanesurfonate (EMS), and nitrosoguanidine (NTG)mutagenesis, a mutant named MRN of the red lactosepositive yeast strain R. acheniorum was obtained. Then, the effects of different growth medium factors on carotenoid production by this mutant at batch-scale level were identified and optimized by means of response surface methodology (RSM) in order to achieve high-level production of β-carotene. The optimum conditions required to achieve the highest level of β-carotene (262.12±1.01mg/L) were determined as follows: whey ultrafiltrate (WU)lactose concentration 55 g/L, pH 5.85, ammonium sulfate concentration 3.5 g/L, temperature 23^oC, and aeration rate 1.56 vvm. The medium optimization resulted in a 6.45-fold increase in volumetric production (262.12±1.01 mg/L) and a 4.62-fold increase in the cellular accumulation (10.69±0.19 mg/g) of β-carotene.