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Determination of 5 Natural Selenium Species in Selenium-enriched Bamboo Shoots Using LC-ICP-MS
Zhanglin Ni,Yihua Liu,Minhua Qu 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
A method for simultaneous determination ofthe selenium species methylselenocysteine (MeSeCys),selenocysteine (SeCys2), selenite (SeIV), selenomethionine(SeMet), and selenates (SeVI) in natural selenium-enrichedbamboo shoots was developed. Samples were extractedusing sonication with a solution of pepsin. Different seleniumspecies were separated using an anion exchange columnwith isocratic elution and a citric acid mobile phase at pH4.7. Separated selenium species were detected as 80Seusing inductively coupled plasma mass spectrometry (ICPMS)in dynamic reaction cell (DRC) gas mode. Themethod achieved acceptable quantitative recoveries of 73.9to 113.4% with relative standard deviations of <5%. Thelimits of detection were 1, 2, 2, 3, and 3 μg/kg (fresh weight)for SeCys2, Se, MeSeCys, SeMet, and Se, respectively. Themethod was simple, rapid, accurate, and useful fordetermination of selenium species in natural seleniumenriched bamboo shoots.