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RISS 인기검색어
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- 저자 : Minglong Zhu (검색결과 1 건)
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The role of microenvironment in aggregation of the 293-human embryonic Kidney cells
Wensong Tan,Liang Zhao,Li Fan,Xu Zhang,Minglong Zhu 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.5
cells. Key factors such as calcium, magnesium and shear stres were investigated in detail and cell aggregate size con-trol was applied to facilitate cell retention improvement. It was found that the concentration of calcium ion affectedthe aggregation of 293-HEK cells drastically and exhibited direct proportion to the average diameter of 293-HEK cellaggregates. Similar effect was also discovered in magnesium but to a lower extend. Results also showed the growthof 293-HEK cells was influenced when the concentrations of calcium or magnesium ions were below 0.1 mol/L,and that was more significant with magnesium. Furthermore, aggregation as well as growth was affected by hard in-trolled to proper size as anticipated and the average sedimentation speed of agregated cells increased about 20 timesto single cells. This is highly advantageous to cell retention improvement either in perfusion cultures or media exchangebefore adenovirus infection by proper control of the cell aggregate size, and thus a high cell concentration and ad-enovirus production potentially can be achieved.
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