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      • SCOPUSKCI등재

        Theoretical Explanation of the Peak Splitting of Tobacco-Specific N-Nitrosamines in HPLC

        Jiang, Juxing,Li, Liangchun,Wang, Mingfeng,Xia, Jianjun,Wang, Wenyuan,Xie, Xiaoguang Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

        During the analyzing processes of the compounds, some researchers are puzzled by the analytical signals for some TSNAs (with or without splitting peaks at various pHs and temperatures). In this work, a detailed theoretical study of structural and thermal properties of the $E/Z$ isomers of TSNAs and the corresponding protonated structures was performed using DFT methods. The calculations showed that the $E$ isomers are almost stable than $Z$ isomers, while the $Z$ isomers would be more stable when in protonation. The calculated results predicted the possibility of separation of their $E$ and $Z$ isomer forms and also showed that protonation affects the dipole moment of molecules significantly (0.1-0.5 to 0.7-2.1 Debye). The calculations agreed well with the experiments that the split-up of the HPLC signal for TSNAs into two peaks are very sensitive to the pH and temperature of the mobile-phase.

      • SCIESCOPUSKCI등재

        Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System

        Li, Jianbo,Jiang, Mingfeng,Wang, Yong Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

        A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific activity greater than 500 mg/L and 4,000 U/mg was obtained. Results at optimal pH and temperature showed recombinant lysozyme has higher lytic activity at pH 6.5 and $45^{\circ}C$. This study demonstrates the successful expression of recombinant lysozyme using the eukaryotic host organism P. pastoris paving the way for protein engineering. Additionally, this study shows the feasibility of subsequent industrial manufacture of the enzyme with this expression system together with a high purity scheme for easy high-yield purification.

      • KCI등재

        A Fast-Transient Repetitive Control Strategy for Programmable Harmonic Current Source

        Wanjun Lei,Cheng Nie,Mingfeng Chen,Huajia Wang,Yue Wang 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1

        The repetitive control (RC) strategy is widely used in AC power systems because of its high performance in tracking period signal and suppressing steady-state error. However, the dynamic response of RC is determined by the fundamental period delay T0 existing in the internal model. In the current study, a (nk ± i)-order harmonic RC structure is proposed to improve dynamic performance. The proposed structure has less data memory and can improve the tracking speed by n/2 times. T0 proves the effectiveness of the (nk ± i)-order RC strategy. The simulation and experiments of (6k ± 1)-order and (4k ± 1)-order RC strategy used in the voltage source inverter is conducted in this study to control the harmonic current source, which shows the validity and advantages of the proposed structure.

      • KCI등재

        Theoretical Explanation of the Peak Splitting of Tobacco-Specific N-Nitrosamines in HPLC

        Juxing Jiang,Liangchun Li,Mingfeng Wang,Jianjun Xia,Wenyuan Wang,Xiaoguang Xie 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

        During the analyzing processes of the compounds, some researchers are puzzled by the analytical signals for some TSNAs (with or without splitting peaks at various pHs and temperatures). In this work, a detailed theoretical study of structural and thermal properties of the E/Z isomers of TSNAs and the corresponding protonated structures was performed using DFT methods. The calculations showed that the E isomers are almost stable than Z isomers, while the Z isomers would be more stable when in protonation. The calculated results predicted the possibility of separation of their E and Z isomer forms and also showed that protonation affects the dipole moment of molecules significantly (0.1-0.5 to 0.7-2.1 Debye). The calculations agreed well with the experiments that the split-up of the HPLC signal for TSNAs into two peaks are very sensitive to the pH and temperature of the mobile-phase.

      • SCIESCOPUSKCI등재

        A Fast-Transient Repetitive Control Strategy for Programmable Harmonic Current Source

        Lei, Wanjun,Nie, Cheng,Chen, Mingfeng,Wang, Huajia,Wang, Yue The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1

        The repetitive control (RC) strategy is widely used in AC power systems because of its high performance in tracking period signal and suppressing steady-state error. However, the dynamic response of RC is determined by the fundamental period delay $T_0$ existing in the internal model. In the current study, a ($nk{\pm}i$)-order harmonic RC structure is proposed to improve dynamic performance. The proposed structure has less data memory and can improve the tracking speed by n/2 times. $T_0$ proves the effectiveness of the ($nk{\pm}i$)-order RC strategy. The simulation and experiments of ($6k{\pm}1$)-order and ($4k{\pm}1$)-order RC strategy used in the voltage source inverter is conducted in this study to control the harmonic current source, which shows the validity and advantages of the proposed structure.

      • Numerical simulation of infill CACB wall cracking subjected to wind loads

        Ruige Li,Yu Gao,Hongjian Lin,Mingfeng Huang,Chenghui Wang,Zhongzhi Hu,Lingyi Jin 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.89 No.5

        The cracking mechanism in ceramsite aerated concrete block (CACB) infill walls were studied in low seismic fortification intensity coastal areas with frequent occurrence of typhoons. The inter-story drifts of an eight-story residential building under wind loads and a seismic fortification intensity of six degrees were analyzed by using the PKPM software. The maximum inter-story drift ratio of the structure in wind load was found to be comparable to that under the seismic fortification intensity of six degrees. However, when accounting for the large gust wind speed of typhoon, the maximum inter-story drift ratio was much larger than that obtained under reference wind load. In addition, the finite element models of RC frames were employed by displacement loading to simulate two scenarios with and without window hole in the CACB infill walls, respectively. The simulation results show no signs of cracking in both the infill walls with window hole and those without window for the inter-story drift caused by seismic loads and the reference wind load. However, both types of infill walls experienced structural creaking when assessing the gust wind pressure recorded from previous typhoon monitoring. It is concluded that an underestimate of wind loads may contribute substantially to the cracking of frame CACB infill walls in low seismic fortification intensity coastal areas. Consequently, it is imperative to adopt wind pressure values derived from gust wind speeds in the design of CACB infill walls within frame structures. Finally, the future research directions of avoiding cracks in CACB filled walls were proposed. They were the material performance improving and building structure optimizing.

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