Prevention and Control of Antimicrobial Resistance and Zoonoses : Surgical Model of Stifle Osteoarthritis Secondary to Medial Patellar Luxation in Dogs

( Md. Rafiqul Alam ),( Hae Beom Lee ),( Sang Youel Park ),( Young Hoon Lee ),( Nam Soo Kim ) 대한인수공통전염병학회 2007 창립총회 및 학술대회 초록집 Vol.2007 No.1

Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs

Md. Rafiqul Alam,Joong Ryong Ji,김민수,김남수 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.3

The levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in synovial fluid (SF) and serum in cases of canine osteoarthritis (OA) were measured. OA was induced by a surgically-created medial patellar luxation in the left stifle of 24 dogs. SF and blood samples were collected at 1.5- and 3-month intervals, respectively. Every 3 months, one dog was euthanatized to collect tissue samples from both stifles. TRAP levels in SF and serum were measured using a spectrophotometer, and TRAP-positive cells in joint tissues were identified by enzyme histochemistry. MMP-2 and TIMP-2 in SF and serum were detected by Western blotting and ELISA, respectively. TRAP in SF from the stifles and serum was significantly increased (p < 0.05) after 3 months. TIMP-2 in SF and serum was significantly decreased (p < 0.05), whereas MMP-2 in SF was significantly increased (p < 0.05) during the progression of OA. Histochemistry revealed an increased number of TRAP-positive cells in tissues from OA-affected joints. Assays measuring TRAP, MMP-2, and TIMP-2 in SF and serum, and methods that detect increased numbers of TRAP-positive cells in the joint tissues can play an important role in identifying the early phases of degenerative changes in canine joint components.

Prevalence of Ruminal Lactic Acidosis and Clinical Assessments of Four Therapeutics in Goats of Bangladesh

Md Mahbubur Rahman,Mohammad Saiful Islam,Adam G.O,Md. Rafiqul Alam,유명조 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.3

Acidosis conditions either acute or chronic following ingestion of excessive amounts of readily fermentedcarbohydrate are great production problems for goat in Bangladesh. This study designed to investigate the prevalenceof lactic acidosis and then response to different therapeutic agents. For this purpose, 1,128 goat were examined atoutdoor District Veterinary Hospital, Faridpur, Bangladseh for treatment of which 40 goats were found positive forlactic acidosis showing 3.55% prevalence of disease. The highest occurrence found in female (4.64%) of over 3 yearsage (4.64%) in indigenous goat (2.7%). For therapeutic assessment the forty affected goats were divided into fourgroups A, B, C and D comprising of 10 animals each. Group A were given magnesium hydroxide 8% w/v at 1 g/kg body weight orally. In group B magnesium hydroxide 8% w/v at a dose as group A combination with 7.5% sodiumbicarbonate at the rate of 0.9 ml/Kg body weight intravenously administered. Goat in group D were treated with mixtureof ginger, nuxvomica, sodium carbonate, cobalt sulphate, dried ferrous sulphate and thiamin mononitrate at the rateof 1 g/kg body weight orally. Goat of group C treated with combination drugs of group A, B and D. The rectaltemperature, pulse rate, respiration rate, was performed before and after treatment. It was found that the highest recoveryin group C with an average period of 21 ± 1.8 hours. It was concluded that lactic acidosis is a common disease ofgoats and its severity can be effectively reduced by using combination drugs.

Hemato-Biochemical Indices of Hanwoo Cattle Raised at Different Altitudes

지중룡,Md. Rafiqul Alam,나종삼,김남수 한국임상수의학회 2011 한국임상수의학회지 Vol.28 No.2

The health status of three groups of Hanwoo steers (n = 157) aged 2-3 years, raised at three different altitudes (600 m, n = 50; 200-400 m, n = 58; plane land, n = 49) and environment with more or less similar management have been evaluated through hematological, biochemical and globulin examinations in order to determine the optimum environment suitable for raising cattle while at the same time minimizing the risk of disease. Five mL of blood samples from each animal were collected by jugular veinipuncture and 2 mL was transferred to a tube containing EDTA for complete blood count (CBC) and 3 mL in lithium heparin for chemistry screening (CS) and immunoassay. Among the CBC parameters a significantly higher white blood cell count (tWBC), total red blood cell count (tRBC), hemoglobin (Hb) and packed cell volume (PCV) were noticed in the high altitude groups, whereas those were lower in the plane land group. In the CS parameters higher levels of aspartate aminotransferase (AST), creatinine, lactate dehydrogenase (LDH) and total bilirubin (TBL) were found in the plane land group, whereas those were lower in the high altitude groups. The total protein (significantly) and globulins were higher in the 600 m group. The results of this study revealed that the overall health status of the Hanwoo cattle based on the hemato-biochemical indices was superior in the highest altitude and inferior in the plane land group but all the parameters were within the reference range in all the groups. Therefore, for recommendation of a suitable environment at an appropriate altitude for raising cattle there need to be further studied along with the hemato-biochemical parameters; considering, breeding, feeding, management, marketing, waste disposal and other factors.

An accidental fatal attack on domestic pigeons by honey bees in Bangladesh

RAHMAN, Md. Mahbubur,LEE, Sei-Jin,KIM, Gi-Beum,YANG, Dong Kwon,ALAM, Md. Rafiqul,KIM, Shang-Jin The Japanese Society of Veterinary Science 2015 The Journal of veterinary medical science Vol.77 No.11

<P>Fatalities among avian species due to multiple bee stings are rare. Sixteen pigeons on a farm in Bangladesh each suffered multiple bee stings. Ten of the pigeons died before treatment, 5 (4–11 stings) died within 12 hr after treatment, and 1 pigeon (only 3 stings) survived. Body temperature, heart rate, respiratory rate, hematocrit, hemoglobin, erythrocytes, thrombocytes, MCV, MCH and MCHC decreased significantly after the incident, but leucocytes, heterophils, basophils, eosinophils, monocytes, ALT, AST, LDH, CK, creatinine, BUN and UA increased markedly. Overall, the hematological and biochemical changes in the bee-stung pigeons were similar to those of mammals; however, avian species may be more sensitive to bee stings than mammals.</P>

Trochleoplasty in Lateral Patellar Luxation in Two Calves

KIM, Nam-Soo,ALAM, Md. Rafiqul,LEE, Jong-Il,PARK, Young-Jae,CHOI, In-Hyuk Japanese Society of Veterinary Science 2005 The Journal of veterinary medical science Vol.67 No.7

<P>Two Holstein Friesian calves were referred to the Animal Medical Center of Chonbuk National University with severe lameness on the hind limb(s), flexed stifle joint and an inability to walk since birth. Based on the clinical history, clinical findings and radiography of the stifle joint, the cases were diagnosed as grade III lateral patellar luxation (LPL). Trochleoplasty along with medial retinacular reinforcement and lateral release were performed in both calves. The calves recovered 3 to 4 weeks after surgery. Trochleoplasty along with medial retinacular reinforcement and lateral release can be an effective technique for the treatment of LPL in calves.</P>

Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition

Pankaj Kumar Jha,Md. Golam Shahi Alam,Md. Abdullah Al Mansur,Mohammad Rafiqul Islam Talukder,Nazmun Naher,A K M Anisur Rahman,David C. Hall,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2020 한국동물생명공학회지 Vol.35 No.2

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 106 sperm), S200 (n = 24; single AI with 200 × 106 sperm), D100 (n = 24; double AI with 100 × 106 sperm) and S100 (n = 18; single AI with 100 × 106 sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 106 sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.

Effects of number of frozen-thawed ram sperm and number of inseminations on fertility in synchronized ewes under field condition

Pankaj Kumar Jha,Md. Golam Shahi Alam,Md. Abdullah Al Mansur,Mohammad Rafiqul Islam Talukder,Nazmun Naher,A. K. M. Anisur Rahman,David C. Hall,Farida Yeasmin Bari 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.2

The effects of the number of frozen-thawed ram sperm per single and double intra-cervical artificial insemination (AI) on fertility in ewes were studied. A total of 89 non-pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; D200 (n = 23; double AI with 200 × 106 sperm), S200 (n = 24; single AI with 200 × 106 sperm), D100 (n = 24; double AI with 100 × 106 sperm) and S100 (n = 18; single AI with 100 × 106 sperm). Ewes were inseminated within 12 to 18 h for single AI and, within 10 to 12 h and 16 to 18 h for double AI after the onset of oestrus. The onset of oestrus ranged from 28 to 76 h (54.33 ± 1.28 h). The high percentage (29.2%) of ewes showed oestrus between 51 to 60 h. The non-return rates were highest in group D200 (56.5%) and differed significantly (p < 0.05) from group S100 (11.1%). No ewes were pregnant in group S100, and the pregnancy rates among the remaining groups did not differ. The mean gestation period was 152.8 ± 0.5 days and no difference was observed among the groups. The lambing and multiple birth rates were 100% in group D200. The single and twin lambing was highest in group D100 (33.3%) and group D200 (83.3%), respectively. Only one triplet lambing and the highest lambing size (2.2 ± 0.2) was recorded in group D200. In conclusion, double AI with 200 × 106 sperm showed comparatively most practical for achieving high pregnancy rates and lambing performances in Bangladeshi ewes under field conditions.

Total hip replacement in a dog

Nam-Soo Kim,Md.Rafiqul Alam,In-Seong Jeong,Jong-Il Lee,In-Hyuk Choi 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.2

An intact male German shepherd dog (4 y, body wt. 35.5 kg) was referred to the Animal Medical Center, Chonbuk National University with severe lameness, pain and gait abnormality on the right hind limb. Survey radiographs of the pelvis revealed dysplasia of the right coxofemoral joint with subluxation. The dog was surgically treated performing total hip replacement (THR) using Modular Biolox Canine Modular THR System. The dog began to bear weight and slowly returned to a normal exercise pattern 2 months after surgery. THR resulted in satisfactory clinical functions with 6 months follow up and no complications were observed. Canine modular THR could be a successful modality for the management of disabling conditions of the coxofemoral joint.