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RISS 인기검색어
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- 저자 : Mandenius, Carl-Fredrik (검색결과 2 건)
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Michael Fritzsche,Carl-Fredrik Mandenius,Joachim Fritzsche,Jonas O. Tegenfeldt 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusionconditions in the microfluidics of the biochip. The autofluorescence from the liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data.
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A Microbore Tubing Based Spiral for Multistep Cell Fractionation
Pasitka, Laura,van Noort, Danny,Lim, Wanyoung,Park, Sungsu,Mandenius, Carl-Fredrik American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.21
<P>Cells were separated with the aid of a multistep spiral fractionation device, utilizing hydrodynamic forces in a spiral tubing. The spiral was fabricated using “off-the-shelf” microbore tubing, allowing for cheap and fast prototyping to achieve optimal cell separation. As a first step, a model system with 20 and 40 μm beads was used to demonstrate the effectiveness of the multistep separation device. With an initial purity of 5%, a separation purity of 83% was achieved after a two-step separation with the addition of 0.1% polyethylene glycol (PEG)-8000. Next, doxorubicin-resistant polyploid giant breast cancer cells (MDA-MB-231) were separated from doxorubicin-sensitive monoploid small breast cancer cells in the same fashion as the beads, resulting in a purity of around 40%, while maintaining a cell viability of more than 90%. Combined with basic cell analytical methods, the hydrodynamic separation principle of the device could be envisaged to be useful for a variety of cell fractionation needs in cell biology and in clinical applications.</P> [FIG OMISSION]</BR>
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