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RISS 인기검색어
- 검색키워드
- 저자 : Mahesh Bule (검색결과 3 건)
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Mahesh V. Bule,Rekha S. Singhal 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.3
Ubiquinone-10 (CoQ10), a vitamin-like lipophilic component of the membrane-bound electron transport system, has a wide range of therapeutic, neutraceutical, and cosmeceutical applications. The objective of this study was to optimize nutritional requirements for production of CoQ10by Paracoccus dinitrificans NRRL B-3785 fermentation. Effect of type and concentration of carbon and nitrogen source on fermentation kinetic parameters were analyzed using logistic and Luedeking-Piret equations. In submerged batch fermentation, yield of CoQ10 was 12.22 mg/L when 40 g/L glycerol was used and specific growth rate (0.056/h) as well as growth associated constant (α=0.680 mg/g)were higher as compared to other concentrations. Ammonium nitrate and proteose peptone at 5 (α=0.677 mg/g; β=0.0072 mg/g·h) and 20 g/L (α=0.806 mg/g; β=0.0074 mg/g·h), respectively, were optimal for CoQ10 production. CoQ10 formation observed to be both growth and nongrowth associated. In optimized medium CoQ10 formation increased considerably from 1.91 to 14.12 mg/L.
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Nagnath R. Jathore,Mahesh V. Bule,Ashwini V. Tilay,Uday S. Annapure 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.4
Levan, a polyfructan which consists of Dfructofuranosyl residues linked predominantly by β-(2,6)linkage as a core chain with some β-(2,1) branch chains have potential applications in the pharmaceutical, food, and cosmetic industries. The present work reports on characterization of levan produced using Pseudomonas fluorescens by Fourier transform infrared spectroscopy (FTIR) and NMR and static fermentation condition optimization using one factor-at-a-time followed by statistical designs. The characterization of levan by FTIR revealed that structure of levan to be homologous to the standard levan sample. 13C and 1H NMR studies further successfully confirmed the levan structure. The optimized medium composition was observed to be (in g/L) sucrose 60; ammonium chloride 1.5; sodium nitrate 2.0, and casein peptone 15.0. The yield of levan was increased significantly from 5.27 to 15.42 g/L when the fermentation was carried out using optimal medium.
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Extraction of p-coumaric acid from agricultural residues and separation using ‘sugaring out’
Pradip B. Dhamole,Shital Chavan,Rashmi G. Patil,Hao Feng,Mahesh Bule,Pallavi Kinninge 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.6
We investigated the extraction of para-coumaric acid (pCA) from different agriculture residues (corn stover, sugarcane bagasse, sorghum stalk, pearl millet stalk, green gram shell, groundnut shell, sesame shell) using sugarcane bagasse alkaline hydrolysis and separation of pCA using sugaring out - a new phase separation method. Primary screening of different feed stocks was by alkaline hydrolysis with 2M NaOH for 6 h at room temperature. Sugarcane baggase resulted into significant amount of pCA (1.1 g/L) and small amount of ferulic acid (FA) (0.23 g/L). The optimized alkaline hydrolysis conditions (2 M NaOH and 16 h) resulted into maximum pCA release of 2.0 g/L. The pCA was separated from alkaline hydrolysate using sugaring out, a two phase separation method that results in aqueous phase and the organic solvent (acetonitrile) phase. Sugaring-out separated more than 90% of the pCA from the alkaline hydrolysate. Results of HPLC using standard pCA and FA showed that the main component of the separated top (organic solvent) phase was pCA rather than FA.
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