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RISS 인기검색어
- 검색키워드
- 저자 : Madhusmita Dhupal (검색결과 4 건)
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Dhupal, Madhusmita,Oh, Jae-Min,Tripathy, Dipti Ranjan,Kim, Soo-Ki,Koh, Sang Baek,Park, Kyu-Sang Dove Medical Press 2018 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.13 No.-
<P><B>Background</B></P><P>Titanium dioxide nanoparticles (TiO<SUB>2</SUB> NPs) represent a scientific breakthrough in the areas of biological and medicinal applications. Interaction of TiO<SUB>2</SUB> NPs with components of innate immune system remains elusive.</P><P><B>Aim</B></P><P>This study explored in vitro immunotoxicity of murine macrophage RAW 264.7 to TiO<SUB>2</SUB> NPs (20 nm, negative charge) and its underlying molecular mechanism by way of immunoredox profiling.</P><P><B>Materials and methods</B></P><P>In this study, chemically synthesized BSA-functionalized TiO<SUB>2</SUB> NPs (20 nm, negative charge) were characterized and immunotoxicity was investigated on RAW 264.7 cells.</P><P><B>Results</B></P><P>We found that reactive oxygen species levels significantly increased with increasing nitric oxide production, whereas depleting endogenous antioxidant super oxide dismutase as well as nuclear factor erythroid 2-related factor 2 (Nrf2) protein levels. Furthermore, NPs exposure increased the expression of apoptotic factors such as BAX, BIM, and PUMA with disruption of mitochondrial membrane potential (Δψ<SUB>m</SUB>) that lead to decrease in immunocytes. Molecular immune profiling revealed the activation of multiple toll-like receptors (TLRs) 4/9/12/13 simultaneously with the phosphorylation of p-p38MAPK and p-SAPK/c-Jun N-terminal kinase (JNK) compared to untreated control.</P><P><B>Conclusion</B></P><P>Collectively, this study shows that the molecular nature of TiO<SUB>2</SUB><SUP>SA20(−)</SUP> NP-induced immunotoxicity in RAW 264.7 macrophage is simultaneous induction of immunocyte apoptosis and multiple TLRs signaling through oxidative stress-dependent SAPK/JNK and p38 mitogen-associated protein kinase activation. This is the first study to address newer molecular mechanism of TiO<SUB>2</SUB><SUP>SA20(−)</SUP> NP-induced immunotoxicity.</P>
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Madhusmita Dhupal,Ming-Hui Zhao,Yong-Nan Xu,Ying-Hua Li,Xiang-Shun Cui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
We have analysed the expression and function of cell cycle and cell death regulator Aven in porcine oocytes. Aven is expressed in a wide variety of adult tissue and cell lines, and there is increased evidence of that its role in cell cycle regulation. The mechanism by which anti apoptotic activity of Aven is poorly understood. Although Aven ortholog have been reported in other mammalian species, no Aven gene has been reported in porcine oocytes. Here we shed light on this issue for the first time in porcine oocytes demonstrating that Aven can be potential oocyte quality marker and a novel inhibitor of caspase activation. Our results revealed there is gradual increase in Aven m-RNA expression upto 4-cell stage and then decreased drastically.This result shows Aven is a maternal gene and is regulated dynamically. In addition. Aven protein localisation,caspase-3 activation and mitochondrial distribution were studied by immunofloroscence. Induction of apoptosis by etoposide drug treatment resulted in changes in Aven localisation within COC, corresponding to caspase-3 activation and altered mitochondrial distribution. These results could be an excellent example for studying the function of Aven and identifying cellular factors that influence its activity,revealing information that may be relevant to human disease.
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Effects of small black soybean product germinated with sulfur on immuno-redox status in C57BL/6 mice
Madhusmita Dhupal,Cheol-Su Kim,Rosa Mistica C. Ignacio,Diptiranjan Tripathy,Soo-Ki Kim,S. K. Kim 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.1
While germinated soybean has been well known for higher content of bioactive substances, and peptides than regular soy bean, still low levels of sulfur containing amino acids to limit its nutritional values. To overcome this, a novel biofortified small black soybean sprout with the processed sulfur (GSSP) was prepared. Using this, we compared the immunomodulatory and anti-oxidant effects with other control groups (NT: small black soy bean non-treated/saline, NSP: normal non-germinated small black soybean product only, GSP: geminated small black soybean product) in C57BL/6 mice model orally fed at 400 or 800 mg/kg/day for two weeks. On immunomodulatory test, we found that GSSP led to the highest splenocyte proliferation as well as the lowest level of nitric oxide (NO) as an inflammatory mediator when compared to NT, NSP and GSP. In line, there was a significant increase of anti-inflammatory cytokine IL-10 level as well as a significant decrease of pro-inflammatory cytokine IL-6, TNF-α, and IFN-γ level in GSSP group when compared to other groups or NSP respectively. Next, redox finding was that with decrease of reactive oxygen species (ROS), there was a significant increase of sulfur-rich protein glutathione peroxidase (GPx) and catalase as endogenous antioxidants in GSP and GSSP group when compared to NT and NSP. Collectively, our study shows that biofortified small black soybean product with processed sulfur (GSSP) has the enhanced immunomodulatory, anti-oxidant effects via immunoredox balancing, importantly implying the promising candidate of soybean based-nutraceutical
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New Insights into ARP2/3 Complex Functional Pathway Promoting Porcine Early Embryo Development
Ying-Hua Li,Madhusmita Dhupal,Yong-Nan Xu 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
Actin-related protein 2/3 (Arp2/3) complex plays a central role in the de novo nucleation of filamentous actin as branches on existing filaments. Small molecule inhibitor CK-666 bind to Arp2/3 complex and inhibit nucleation, but their modes of action are unknown. Here, we shed light on the functional aspects of Arp2/3 by CK-666 inhibitor on porcine early embryo development. Our data indicated that CK-666 destabilizes the complex, blocking movement of the Arp2 and Arp3 subunits, causing actin degradation and failure of cell division which results in failure of early embryo development. Our results also shows there is decrease in mRNA expression of ICM and trophectoderm related genes such as Sox2, Pou5f1, Nanog. These results provided key insights into the relationship between early embryo dvelopement and critical role of Arp2/3 complex.
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