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- 저자 : Liu Zijin (검색결과 2 건)
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Zhenghua Qian,Jianan Song,Zijin Liu,Zonglin Peng 한국고분자학회 2019 Macromolecular Research Vol.27 No.11
To fully utilize the fascinating comprehensive properties of graphene oxide (GO) and carbon nanotubes (CNTs), GO was used to promote the dispersion of carboxylated multi-walled carbon nanotubes (CC) in rubber matrix. Additionally, carboxylated acrylonitrile butadiene rubber (xNBR) was used to enhance the interfacial interaction between the styrene-butadiene rubber (SBR) and the GO/CC hybrid fillers for the formation of hydrogen bonds between the oxygenated functional groups of GO/CC hybrid fillers and the carboxyl groups of xNBR. Moreover, the interfacial interaction was investigated by Fourier transform infrared spectroscopy and further proved by differential scanning calorimetry. As a result, the mechanical property and thermal conductivity of SBR composites were improved significantly compared with the neat SBR vulcanizate, which were much higher than those of the SBR composites without xNBR. In contrast to adding GO/CNTs directly to the rubber matrix, enhancing the interfacial interaction between GO/CC hybrid fillers and rubber matrix as demonstrated herein is a valuable strategy to prepare rubber composites with remarkable comprehensive properties.
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Cao Hongli,Zhang Guosheng,Ma Hui,Xue Zhongwen,Huo Ran,Wang Kun,Liu Zijin 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.1
Refractory infections, such as hospital-acquired pneumonia, can be better diagnosed with the assistance of precise methicillin-resistant Staphylococcus aureus (MRSA) testing. However, traditional methods necessitate high-tech tools, rigorous temperature cycling, and the extraction of genetic material from MRSA cells. Herein, we propose a sensitive, specific, and extraction-free strategy for MRSA detection by integrating allosteric probe-based target recognition and exonuclease-III (ExoIII)-enhanced color reaction. The penicillin-binding protein 2a (PBP2a) aptamer in the allosteric probe binds with MRSA to convert protein signals to nucleic acid signals. This is followed by the DNA polymerase-assisted target recycle and the production of numerous single-strand DNA (ssDNA) chains which bind with silver ion (Ag+ ) aptamer to form a blunt terminus that can be identified by Exo-III. As a result, the Ag+ aptamer pre-coupled to magnetic nanoparticles is digested. After magnetic separation, the Ag+ in liquid supernatant catalyzes 3,3’,5,5’-tetramethylbenzidine (TMB) for a color reaction. In addition, a concentration of 54 cfu/mL is predicted to be the lowest detectable value. Based on this, our assay has a wide linear detection range, covering 5 orders of magnitude and demonstrating a high specificity, which allows it to accurately distinguish the target MRSA from other microorganisms.
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