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RISS 인기검색어
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- 저자 : Lin Qiannan (검색결과 3 건)
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Hu, Zhe,Liu, Qiannan,Zhang, Kai,Zhou, Limin,Li, Lin,Chen, Mingzhe,Tao, Zhanliang,Kang, Yong-Mook,Mai, Liqiang,Chou, Shu-Lei,Chen, Jun,Dou, Shi-Xue American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.42
<P>Dual ion batteries based on Na<SUP>+</SUP> and PF<SUB>6</SUB><SUP>-</SUP> received considerable attention due to their high operating voltage and the abundant Na resources. Here, cheap and easily obtained graphite that served as a cathode material for dual ion battery delivered a very high average discharge platform (4.52 V vs Na<SUP>+</SUP>/Na) by using sodium hexafluorophosphate in propylene carbonate as electrolyte. Moreover, the all-carbon dual ion batteries with graphite as cathode and hard carbon as anode exhibited an ultrahigh discharge voltage of 4.3 V, and a reversible capacity of 62 mAh·g<SUP>-1</SUP> at 40 mA·g<SUP>-1</SUP>. Phase changes have been investigated in detail through in situ X-ray diffraction and in situ Raman characterizations. The stable structure provides long life cycling performance, and the pseudocapacitance behavior also demonstrates its benefits to the rate capability. Thus, dual ion batteries based on sodium chemistry are very promising to find their applications in future.</P> [FIG OMISSION]</BR>
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( Hang Liu ),( Linan Xie ),( Jing Wang ),( Qiannan Guo ),( Shengnan Yang ),( Pei Liang ),( Chengshu Wang ),( Min Lin ),( Yuquan Xu ),( Liwen Zhang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
Beauveria bassiana infects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in B. bassiana remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of B. bassiana ARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth (Plutella xylostella (Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.
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Development of Enzymatic Recombinase Amplification Assays for the Rapid Visual Detection of HPV16/18
Ding Ning,Qi Wanwan,Wu Zihan,Zhang Yaqin,Xu Ruowei,Lin Qiannan,Zhu Jin,Zhang Huilin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Human papillomavirus (HPV) types 16 and 18 are the major causes of cervical lesions and are associated with 71% of cervical cancer cases globally. However, public health infrastructures to support cervical cancer screening may be unavailable to women in low-resource areas. Therefore, sensitive, convenient, and cost-efficient diagnostic methods are required for the detection of HPV16/18. Here, we designed two novel methods, real-time ERA and ERA-LFD, based on enzymatic recombinase amplification (ERA) for quick point-of-care identification of the HPV E6/E7 genes. The entire detection process could be completed within 25 min at a constant low temperature (35–43°C), and the results of the combined methods could be present as the amplification curves or the bands presented on dipsticks and directly interpreted with the naked eye. The ERA assays evaluated using standard plasmids carrying the E6/E7 genes and clinical samples exhibited excellent specificity, as no cross-reaction with other common HPV types was observed. The detection limits of our ERA assays were 100 and 101 copies/μl for HPV16 and 18 respectively, which were comparable to those of the real-time PCR assay. Assessment of the clinical performance of the ERA assays using 114 cervical tissue samples demonstrated that they are highly consistent with real-time PCR, the gold standard for HPV detection. This study demonstrated that ERA-based assays possess excellent sensitivity, specificity, and repeatability for HPV16 and HPV18 detection with great potential to become robust diagnostic tools in local hospitals and field studies.
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