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RISS 인기검색어
- 검색키워드
- 저자 : Lili Matyas (검색결과 2 건)
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Ferenc Kiss,Erika Sajtos,Lili Matyas,Zsuzsanna Magyar,Istvan Furka,Iren Miko,Norbert Nemeth 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.2
Viscosity of the suspending medium can be a determinant contributor to the effective measurements of red blood cell deformability using ektacytometry. Deformability of erythrocyte is known to show inter-species and gender differences, therefore the evaluation of the effects and the standardized usage of the suspending media are important in animal experiments. For evaluating the differences using various viscosity media, we aimed to test male and female Sprague-Dawley rats and beagle dogs. Blood samples were collected and red blood cell deformability was tested by a RheoScan-D200 slit-flow ektacytometer using parallel measurements in PVP solutions at viscosity of 15, 20 and 30mPa.s. The lowest elongation index values were measured in 15mPa.s solution in both species and genders. The inter-species differences were obvious: rats had higher elongation index values. Gender differences were found only in 20 and 30mPa.s samples: in rats males, while in dogs females had better red blood cell deformability. The standard usage of PVP solutions is essential for comparative studies, because the results obtained from different viscosity PVP solutions are not comparable to each other. In Sprague-Dawley rats and beagle dogs the relatively less viscous media (<20mPa.s) may result in less informative data.
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Are there arterio-venous differences of blood micro-rheological variables in laboratory rats?
Timea Hever,Ferenc Kiss,Erika Sajtos,Lili Matyas,Norbert Nemeth 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.1
In animal experiments blood samples are often taken from various parts of the circulation. Although several variables including blood gas parameters are known to alter comparing arterial to venous system, arterio-venous (A-V) differences of blood micro-rheological variables (erythrocyte deformability and aggregation) tested by ektacytometry and aggregometry are not completely known in laboratory rats. In 12 outbred rats we investigated red blood cell deformability (RheoScan-D200 slit-flow ektacytometer), red blood cell aggregation (Myrenne MA-1 erythrocyte aggregometer), hematological variables (Sysmex F-800 microcell counter), blood pH and blood gases (ABL555 Radiometer Copenhagen) in blood samples taken parallel from the abdominal aorta and from the caudal caval vein. Blood pH did not differ, blood gas partial tensions showed physiological A-V differences, as it was expected. White blood cell count, red blood cell count and hematocrit were significantly higher in samples from the caval vein. Erythrocyte aggregation values (at 3 1/s shear rate) were significantly higher in samples taken from the abdominal aorta. Erythrocyte deformability (elongation index) did not show obvious A-V differences. Arterio-venous hemorheological differences -mostly of erythrocyte aggregation- can be found in rats, thus, the standardization of the studies and planning appropriate control measurements are necessary for safe evaluation of the obtained results.
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