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RISS 인기검색어
- 검색키워드
- 저자 : Lianshun Feng (검색결과 2 건)
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Imaging Cell Surface Glycans ofCardiomyocytes in Intact Rat Heart
Jie Rong,Jing Han,Lianshun Feng,Yanhong Tan,Qiwei Wang,Yingying Chen,Shiqiang Wang,Xing Chen 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Glycosylation is essential for proper cell signaling and embryonic development. Changes in glycosylation are often a hallmark of disease states. Cardiovascular disease has become one of the top causes of death. Many studies using rat models have related heart diseases and regulations of heart physiological functions to altered glycosylations. However, the dynamic regulation and molecular mechanism of glycosylation in heart are not clear. Rat is an important model organism for human cardiovascular disease studies. Isolated intact rat hearts are often used in physiological and pathological studies. In situ imaging biomolecules in intact heart avoid possible damage of myocyte and biased loss of cardiac myocytes during enzymatic isolation. Here, we applied the metabolic glycan labeling technique for imaging sialylation and O-linked glycosylation in living cardiomyocytes and intact rat hearts. The effects of unnatural sugars on the function of cardiomyocytes and hearts were evaluated. We found unnatural sugars did not perturb the function of cardiomyocytes and hearts. Glycan imaging revealed an interesting distribution pattern: sialic acid or O- linked glycan enriched at intercalated discs of adult rat cardiomyocytes. We also observed that the glycosylations of cardiomyocytes were altered in isoproterenol-induced cardiac hypertrophy rat models. This work extends the application of metabolic glycan labeling technique to probe glycosylations in rats and provides the first example of using this technique for in situ cellular glycans imaging in mammalian model organisms. The biological significance of altered glycosylations in cardiac hypertrophy heart is under investigation in our lab.
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Cell-Selective Metabolic Glycan Labeling Based on Ligand-Targeted Liposomes
Ran Xie,Senlian Hong,Lianshun Feng,Jie Rong,Xing Chen 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Cell-surface glycans play key roles in mediating various molecular recognition events; aberrant glycosylation is implicated in disease progression. Therefore, probing the dynamic changes of glycan biosynthesis and structures is of great importance for augmenting our understanding of glycobiology and improving disease diagnosis and therapeutics. The metabolic glycan labeling was an appealing approach to incorporate specially designed carbohydrate analogs into the glycans, which enables the detection and imaging of the glycans in cells and living animals. However, one major bottleneck of this method is cell- type selectivity. Herein, we present the cell-specific metabolic glycan labeling using ligand-targeted liposomes to deliver unnatural sugars to target cells in a cell-surface receptor dependent manner. In this strategy, sugar analogs are encapsulated in ligand-targeted liposomes. The ligands bind to specific cell-surface receptors that are only expressed or up-regulated in target cells, which mediate the intracellular delivery of azidosugars via endocytosis. The delivered azidosugars are metabolically incorporated into cell-surface glycans and detected or imaged using a bioorthogonal reaction. The application of this strategy includes the facile introduction of myriad alternative ligands, as well as the cell-specific or tissue-specific imaging and detection of glycosylation in vivo.
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