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Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction
Zheng, Lian-Ying,Xiao, Yu-Liang 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1
Chitosanase obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE cellulose with glutaraldehyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1g DEAE cellulose was treated with 5㎖ 5% glutaraldehyde solution; then 2.3㎎ chitosanase was immobilized on the carrier. The optimal temperature and pH was 60℃ and 4.0, and the K_(m) value was 18.87g/L. Under optimal conditions, the activity of immobilized enzyme is 1.5U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal temperature and K_(m) value increased (from 50℃ to 60℃, from 2.49g/L to 18.87g/L), whereas the optimal pH was reduced (from 5.0 to 4.0). The enzyme activity loss was less than 20% after 10 times batch reaction; the immobilized enzyme showed good operation stability.
Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction
Lian-Ying Zheng,Yu-Liang Xiao 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1
obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE celulose with glutaralde-hyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose wastreated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier. The optimaltemperature and pH was 60oC and 4.0, and the Km value was 18.87 g/L. Under optimal conditions, the activity of im-mobilized enzyme is 1.5 U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal tem-perature and Km value increased (from 50oC to 60oC, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was re-duced (from 5.0 to 4.0). The enzyme activity los was les than 20% after 10 times batch reaction; the imobilizedenzyme showed good operation stability.