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Yamaoka, Yasuyo,Achard, Dorine,Jang, Sunghoon,Legé,ret, Bertrand,Kamisuki, Shogo,Ko, Donghwi,Schulz‐,Raffelt, Miriam,Kim, Yeongho,Song, Won‐,Yong,Nishida, Ikuo,Li‐,Beisson, Yon BLACKWELL 2016 PLANT BIOTECHNOLOGY JOURNAL Vol.14 No.11
<P><B>Summary</B></P><P>Despite a strong interest in microalgal oil production, our understanding of the biosynthetic pathways that produce algal lipids and the genes involved in the biosynthetic processes remains incomplete. Here, we report that <I>Chlamydomonas reinhardtii Cre09.g398289</I> encodes a plastid‐targeted 2‐lysophosphatidic acid acyltransferase (CrLPAAT1) that acylates the <I>sn</I>‐2 position of a 2‐lysophosphatidic acid to form phosphatidic acid, the first common precursor of membrane and storage lipids. <I>In vitro</I> enzyme assays showed that CrLPAAT1 prefers 16:0‐CoA to 18:1‐CoA as an acyl donor. Fluorescent protein‐tagged CrLPAAT1 was localized to the plastid membrane in <I>C. reinhardtii</I> cells. Furthermore, expression of CrLPAAT1 in plastids led to a > 20% increase in oil content under nitrogen‐deficient conditions. Taken together, these results demonstrate that CrLPAAT1 is an authentic plastid‐targeted LPAAT in <I>C. reinhardtii</I>, and that it may be used as a molecular tool to genetically increase oil content in microalgae.</P>