http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ramesh Prasad Pandey,Le Tai Feng,Mi Kyoung Kim,Jae Kyung Sohng 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
A novel GT1 family glycosyltransferase, YjiC from Bacillus licheniformis ATCC 14580 has been PCR amplified, cloned, and expressed in E. coli BL21 (DE3) expression host. The expression of 396 amino acid long gene generated ~45 kDa N-terminal his-tag protein. The protein has been purified and used for the in vitro glycosylation of phloretin. Moreover, the enzyme was also applied for the in vivo glycosylation of the same phloretin molecule. The in vitro and in vivo study found that YjiC can glycosylate at different positions of hydroxyl groups of phloretin molecule very efficiently with conversion rate of ~98%. Four different products has been identified from the in vitro as well as in vivo reaction as phloretin-2’-glucoside, phloretin-4’-glucoside, and two different diglucosides of phloretin. All the products have been confirmed by TLC, HPLC, LC-MS, and former two products has been further confirmed by NMR analysis.