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      • SCOPUSKCI등재

        The Influence of a Second Metal on the Ni/SiC Catalyst for the Methanation of Syngas

        ( Lan Lan Song ),( Yue Yu ),( Xiao Xiao Wang ),( Guoq Jang Jin ),( Ying Yong Wang ),( Xiang Yun Guo ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.5

        The catalytic performance of silicon carbide supported nickel catalysts modified with or without secondmetal (Co, Cu and Zn) for the methanation of CO has been investigated in a fixed-bed reactor using a feed consisting of25% CO and 75% H2 without any diluent gas. It has been found that the introduction of Co species can clearly improvethe catalytic activity of Ni/SiC catalyst, whereas the addition of Cu or Zn can result in a significant decrease in the catalyticactivity. The characterizations by means of XRD, TEM, XPS, CO-TPD and H2-TPR indicate that the addition ofCo could decrease the particle size of active metal, increase active sites on the surface of methanation catalyst, improvethe chemisorption of CO and enhance the reducibility of methanation catalysts. Additionally, the special interaction betweenCo species and Ni species is likely favorable for the dissociation of adsorbed CO on the surface of catalyst, and this mayalso contribute to the high activity of 5Co-Ni/SiC catalyst for CO methanation reaction. For 5Cu-Ni/SiC catalyst and 5Zn-Ni/SiC catalyst, Cu and Zn species could cover partial nickel particles and decrease the chemisorption amount of CO. These could be responsible for the low methanation activity. In addition, a 150h stability test under 2 MPa and 300 oCshowed that 5Co-Ni/SiC catalyst was very stable for CO methanation reaction.

      • SCIESCOPUSKCI등재

        Color Stability of Chinese-Style Sausage Inoculated with Staphylococcus Carnosus and Staphylococcus Xylosus

        Guo, Hsiu-Lan,Liu, Deng-Cheng,Chen, Ming-Tsao Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.4

        This study investigate the effects of starter cultures on the color stability of Chinese-style sausage. The samples were inoculated with $10^7cfu\;g^{-1}$ meat of either Staphylococcus carnosus or Staphylococcus xylosus. After mixing, curing at either $4^{\circ}C$ or $20^{\circ}C$ for 20 h and then drying at $50^{\circ}C$ for 5 h, the samples were then either vacuum packed or hung at $4^{\circ}C$ and $25^{\circ}C$ (85% R. H.). The pH, nitrite content, nitrosyl pigment content, metmyoglobin and L-, a-, b- values were measured. The pH value still remained above 6.0 during storage. Nitrite residue of all samples decreased after storage at $25^{\circ}C$ for 7 days. The samples inoculated with S. carnosus and S. xylosus had higher nitrite content (20.9-34.7 ppm) than the control (p<0.05). Samples inoculated with S. carnosus and S. xylosus had higher nitrosyl pigment content and lower metmyoglobin content than those of the control. The L- and b- values of all samples decreased but the a- values increased with storage time. The result suggested that S. carnosus and S. xylosus starter cultures be used to improve color stability of Chinese-style sausage.

      • KCI등재

        Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

        Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.

      • SCOPUSKCI등재

        Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

        Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,Huang, Li-Li,Zhang, Bo-Ning,Pang, Xiao-Bin,Liu, Ben-Ye,Ma, Lan-Qing,Wang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.

      • KCI등재

        대량 맞춤옷장 표준화 유닛 캐비닛의 기능 모듈 및 구조 디자인에 관한 연구 : 한샘(HANSSEM) 맞춤옷장을 중심으로

        곽란(Guo, Lan),강재철(Kang, Jae Cheol) 한국디자인리서치학회 2020 한국디자인리서치 Vol.5 No.1

        대량 맞춤(Mass Customization, 약칭 MC )가구는 표준화 유닛 캐비닛 + 비표준화 맞춤 캐비닛 으로 조합된 디자인 방식으로 구성되었으며 그 맞춤화한 상품과 서비스, 대량 생산방식, 공간과 완전 통일된 접합 형태는 신속히 정보시대 주된 가구제작 방식으로 되었다. 이를 기초로, 본 논문은 대량 맞춤옷장 중 표준화 유닛 캐비닛의 디자인을 중심으로 모듈화 디자인방법을 이용하여 표준화 유닛 캐비닛의 기능 모듈과 디자인의 조합에 대해 연구하였다. 연구에서 맞춤옷장 표준화 원칙의 계열화, 통용화와 조합화 원칙에 근거하여 모듈화 디자인방법과 결합하여 표준화 유닛 캐비닛의 표준화, 기능 및 구조 간의 관계 구조도를 구축하였다. 마지막으로 한국의 가구브랜드인 한샘 의 맞춤옷장을 사례로 하여 기능과 연결구조의 측면에서 현존하는 두 개의 표준화 계열에 대해 모듈 분석하였으며 표준화 유닛 캐비닛의 기능과 구조 디자인에 대해 연구하였다. 결론적으로 대량 맞춤옷장은 기능 모듈의 배치를 통해 디자인과 결합하여 상품의 내부적 다양화를 줄이고 외부적 다양화를 증가하는 표준화된 디자인 목적에 도달하는 동시에 맞춤옷장의 표준화 유닛 캐비닛 디자인에서 기능 모듈과 구조 디자인에 적응성 관계가 있다는 결론을 얻었다. 본 연구를 통해 대량 맞춤가구의 실천과 맞춤옷장 기능 모듈화의 개발에 일정한 참고적 자료를 제공할 수 있기를 희망한다. Mass Customization (MC) wardrobe is the design which through the combination of standardized unit cabinet + non-standardized customized cabinet . It has rapidly developed as the manufacturing mainstream mode in the information age furniture for its customized products and services, mass production mode, complete and unified space joint form. This paper studies the design and combination of functional modules in the standardized unit cabinet with the modular design method, centering on the design of standardized unit cabinet in the mass customization closet. According to the principle of serialization, generalization and combination of the standardization principle of customized wardrobe, the structural diagram of the function, structure and standardization of the cabinet of standardized unit is established by combining the modular design method. Finally, taking the customized wardrobe of Korean furniture brand Hanssem company as an example, this paper divided the modules of its two existing standardized series from the dimension of function and connection structure, and studied the functional and structural design of standardized unit cabinet. Conclusion: Hanssem can make use of the configuration combination design of functional modules to achieve the standardized design purpose of reducing the internal diversification of products and increasing the external diversification of products. In addition, there is an adaptive relationship between functional modules and structural design in the standardized unit cabinet body design of customized wardrobes. It is hoped that this research can have certain reference value to the practice of mass customization furniture and the development of customized wardrobe function module.

      • KCI등재

        DsLCYB Directionally Modulated β-Carotene of the Green Alga Dunaliella salina under Red Light Stress

        Lan Yanhong,Song Yao,Guo Yihan,Qiao Dairong,Cao Yi,Xu Hui 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12

        Carotenoids, which are natural pigments found abundantly in wide-ranging species, have diverse functions and high industrial potential. The carotenoid biosynthesis pathway is very complex and has multiple branches, while the accumulation of certain metabolites often affects other metabolites in this pathway. The DsLCYB gene that encodes lycopene cyclase was selected in this study to evaluate β-carotene production and the accumulation of β-carotene in the alga Dunaliella salina. Compared with the wild type, the transgenic algal species overexpressed the DsLCYB gene, resulting in a significant enhancement of the total carotenoid content, with the total amount reaching 8.46 mg/g for an increase of up to 1.26-fold. Interestingly, the production of α-carotene in the transformant was not significantly reduced. This result indicated that the regulation of DsLCYB on the metabolic flux distribution of carotenoid biosynthesis is directional. Moreover, the effects of different light-quality conditions on β-carotene production in D. salina strains were investigated. The results showed that the carotenoid components of β-carotene and β-cryptoxanthin were 1.8- fold and 1.23-fold higher than that in the wild type under red light stress, respectively. This suggests that the accumulation of β-carotene under red light conditions is potentially more profitable.

      • Betaine Effects on Morphology, Proliferation, and p53-induced Apoptosis of HeLa Cervical Carcinoma Cells in Vitro

        Guo, Yu,Xu, Li-Sha,Zhang, Ding,Liao, Ya-Ping,Wang, Hai-ping,Lan, Zhi-Hui,Guan, Wei-Jun,Liu, Chang-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

        Objectives: To investigate the effects of betaine on HeLa cell growth and apoptosis and molecular mechanisms. Materials and Methods: Concentrations of 0.1, 1.0, 5.0, 20.0, 100.0 mg/ml of betaine were used to evaluate the anticancer efficacy for HeLa cells respectively, and MCF-10A was also detected as a normal diploid cell control. Results: We found that proliferation of HeLa cells was inhibited significantly upon exposure to increasing betaine levels with the MTT test (p<0.05). The percentage of S phase cells in the low dose groups (<5mg/ml) were distinctly higher than in high dose groups, and the rates of Sub-G1 phase were the opposite (p<0.01); A high concentration of betaine (>5.0mg/ml) significantly promoted the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups were slightly higher than the control group (p<0.05) and there were obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 could be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints. Conclusions: Our data showed that betaine could promote HeLa cells proliferation in vitro at low concentrations. In contrast, high concentrations could significantly inhibit cell growth and migration, and induce apoptosis of HeLa cells through caspase 3 signaling and further promoted necrosis. This might imply that betaine exhibits tumoricidal effects and acts as a biological response modifier in cancer treatment by inducing apoptosis and cell cycle arrest in a dose and time-dependent manner.

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