A Novel Molecular Grading Model: Combination of Ki67 and VEGF in Predicting Tumor Recurrence and Progression in Non-invasive Urothelial Bladder Cancer

Chen, Jun-Xing,Deng, Nan,Chen, Xu,Chen, Ling-Wu,Qiu, Shao-Peng,Li, Xiao-Fei,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Purpose: To assess efficacy of Ki67 combined with VEGF as a molecular grading model to predict outcomes with non-muscle invasive bladder cancer (NMIBC). Materials: 72 NMIBC patients who underwent transurethral resection (TUR) followed by routine intravesical instillations were retrospectively analyzed in this study. Univariate and multivariate analyses were performed to confirm the prognostic values of the Ki67 labeling index (LI) and VEGF scoring for tumor recurrence and progression. Results: The novel molecular grading model for NMIBC contained three molecular grades including mG1 (Ki67 $LI{\leq}25%$, VEGF $scoring{\leq}8$), mG2 (Ki67 LI>25%, VEGF $scoring{\leq}8$; or Ki67 $LI{\leq}25%$, VEGF scoring > 8), and mG3 (Ki67 LI > 25%, VEGF scoring > 8), which can indicate favorable, intermediate and poor prognosis, respectively. Conclusions: The described novel molecular grading model utilizing Ki67 LI and VEGF scoring is helpful to effectively and accurately predict outcomes and optimize personal therapy.

Modeling, Preparation, and Elemental Doping of Li₇La₃Zr₂O₁₂ Garnet-Type Solid Electrolytes: A Review

Cao, Shiyu,Song, Shangbin,Xiang, Xing,Hu, Qing,Zhang, Chi,Xia, Ziwen,Xu, Yinghui,Zha, Wenping,Li, Junyang,Gonzale, Paulina Mercedes,Han, Young-Hwan,Chen, Fei The Korean Ceramic Society 2019 한국세라믹학회지 Vol.56 No.2

Recently, all-solid-state batteries (ASSBs) have attracted increasing interest owing to their higher energy density and safety. As the core material of ASSBs, the characteristics of the solid electrolyte largely determine the performance of the battery. Thus far, a variety of inorganic solid electrolytes have been studied, including the NASICON-type, LISICON-type, perovskite-type, garnet-type, glassy solid electrolyte, and so on. The garnet Li₇La₃Zr₂O₁₂ (LLZO) solid electrolyte is one of the most promising candidates because of its excellent comprehensively electrochemical performance. Both, experiments and theoretical calculations, show that cubic LLZO has high room-temperature ionic conductivity and good chemical stability while contacting with the lithium anode and most of the cathode materials. In this paper, the crystal structure, Li-ion transport mechanism, preparation method, and element doping of LLZO are introduced in detail based on the research progress in recent years. Then, the development prospects and challenges of LLZO as applied to ASSBs are discussed.

Study on Multi-Field Coupled Evolution Mechanism of Laser Irradiated 40Cr Steel Quenching Process Based on Phase Change Induced Plasticity

Chang Li,Hexin Gao,Xinxue Chen,Zhaotai Liu,Xing Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

40Cr is essential to steel for the manufacture of hardened gears, and optimizing its laser quenching process will be of greatsignificance for gear wear and life extension. However, the complete reliance on the experimental trial-and-error approachdoes not effectively reveal the mechanism of the evolution of the laser quenching process in disk laser. Numerical simulationprovides an effective way to obtain the transient evolution of the laser quenching process. In this paper, the temperaturechanges physical parameters of the quenched material are calculated based on the CALPHAD method. Establish a thermoelastic–plastic multi-field coupling model for the laser hardening process of the disk laser 40Cr gear steel. Perform thenumerical calculations on the transient temperature, martensite phase fraction, and the transformation-induced plasticitystress evolution of the 40Cr laser hardening process. Focus on the mechanism of the mutual coupling between phase changebehavior and plastic strain. Calculations show that there is heat accumulation during quenching, with a peak temperatureof 1448 K. Plastic stress has a “hump” distribution, with a peak value of 834 MPa, which is 1.25 mm from the center ofthe focal point. The experimental analyses of the 40Cr gear steel quenching microstructure and hardness distribution stateby the Axioskop2 scanning electron microscope, the KEYENCE VH-Z100R super depth of field 3D microscope, and theQ10M microhardness tester reveal the mechanical behavior of the material and the phase hardening law during the quenchingprocess. That provided an essential theoretical basis for effective prediction on quenching residual stress and optimizingindustrial production parameters.

Effect of Bee Venom Acupuncture on Oxaliplatin-Induced Cold Allodynia in Rats

Lim, Bong-Soo,Moon, Hak Jin,Li, Dong Xing,Gil, Munsoo,Min, Joon Ki,Lee, Giseog,Bae, Hyunsu,Kim, Sun Kwang,Min, Byung-Il Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Oxaliplatin, a chemotherapy drug, often leads to neuropathic cold allodynia after a single administration. Bee venom acupuncture (BVA) has been used in Korea to relieve various pain symptoms and is shown to have a potent antiallodynic effect in nerve-injured rats. We examined whether BVA relieves oxaliplatin-induced cold allodynia and which endogenous analgesic system is implicated. The cold allodynia induced by an oxaliplatin injection (6 mg/kg, i.p.) was evaluated by immersing the rat's tail into cold water (4°C) and measuring the withdrawal latency. BVA (1.0 mg/kg, s.c.) at Yaoyangguan (GV3), Quchi (LI11), or Zusanli (ST36) acupoints significantly reduced cold allodynia with the longest effect being shown in the GV3 group. Conversely, a high dose of BVA (2.5 mg/kg) at GV3 did not show a significant antiallodynic effect. Phentolamine (<I><I>α</I></I>-adrenergic antagonist, 2 mg/kg, i.p.) partially blocked the relieving effect of BVA on allodynia, whereas naloxone (opioid antagonist, 2 mg/kg, i.p.) did not. We further confirmed that an intrathecal administration of idazoxan (<I><I>α</I></I><SUB>2</SUB>-adrenergic antagonist, 50 <I><I>μ</I></I>g) blocked the BVA-induced anti-allodynic effect. These results indicate that BVA alleviates oxaliplatin-induced cold allodynia in rats, at least partly, through activation of the noradrenergic system. Thus, BVA might be a potential therapeutic option in oxaliplatin-induced neuropathy.</P>

Remote sensing of early-stage green tide in the Yellow Sea for floating-macroalgae collecting campaign

Xing, Qianguo,Wu, Lingling,Tian, Liqiao,Cui, Tingwei,Li, Lin,Kong, Fanzhou,Gao, Xuelu,Wu, Mengquan Elsevier 2018 Marine pollution bulletin Vol.133 No.-

<P><B>Abstract</B></P> <P>The world's largest green tide originated from the Jiangsu Shoal of the Yellow Sea was due to fast reproduction of floating green macroalgae (<I>Ulva prolifera</I>). It brought significant impacts on marine environment and ecosystem in the Yellow Sea. In this study, we examined the expansion of green tide from the Jiangsu Shoal during the period from 29 April to 25 June 2016. Using high-resolution satellite images, we revealed a declined growth rate during the northward drifting of early-stage green tide for the first time, i.e., the green tide had higher growth rate (up to 25% per day) in the turbid waters of the Jiangsu Shoal in May and a lower growth rate (low to 3% per day) in the relatively clear waters in the middle of the western Yellow Sea in June, which suggests that water clarity might not be the key factor controlling the growth rate of the floating macroalgae in the surface waters under natural conditions. The high growth rate led to shortened time windows for controlling the green tide by employing macroalgae collecting campaigns at the initial sites of the green tide, which was no more than 14 days in the 2016 case.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using high-resolution satellite image for detecting early-stage green tide </LI> <LI> Found changing growth rate of green tide </LI> <LI> Assessed the countermeasure of collecting floating-macroalgae at the initial sites </LI> </UL> </P>

Hepatitis B Virus DNA Negativity Acts as a Favorable Prognostic Factor in Hepatocellular Carcinoma Patients

Li, Xing,Zhong, Xiang,Chen, Zhan-Hong,Xing, Yan-Fang,Wu, Dong-Hao,Chen, Jie,Ma, Xiao-Kun,Lin, Qu,Wen, Jing-Yun,Wei, Li,Wang, Tian-Tian,Ruan, Dan-Yun,Lin, Ze-Xiao,Wu, Xiang-Yuan,Dong, Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: This retrospective study was aimed to investigate the efficacy of prophylactic agents in hepatocellular carcinoma (HCC) patients receiving TACE and compare the difference between lamivudine and entecavir. Materials and Methods: A consecutive series of 203 HBV-related HCC patients receiving TACE were analyzed including 91 patients given prophylactic agents. Virologic events, defined as an increase in serum HBV DNA level to more than 1 log10 IU/ml higher than the nadir level, hepatitis flares due to HBV reactivation and progression free survival (PFS) were the main endpoints. Results: Some 48 (69.6%) reached virologic response. Prophylaxis significantly reduced virologic events (8.8% vs 58.0%, p=0.000) and hepatitis flares (1.1% vs 13.4%, p=0.001). Patients presenting undetectable HBV DNA levels displayed a significantly improved PFS as compared to those who never achieved undetectable HBV DNA. Prophylaxis and e-antigen positivity were the only significant variables associated with virologic events. In addition, prophylaxis was the only independent protective factor for hepatitis flares. Liver cirrhosis, more cycles of TACE, HBV DNA negativity, a lower Cancer of the Liver Italian Program score, non-metastasis and no hepatitis flares were protective factors for PFS. Prophylactic lamivudine demonstrated similar efficacy as entecavir. Conclusions: Prophylactic agents are efficacious for prevention of HBV reactivation in HCC patients receiving TACE. Achievement of undetectable HBV DNA levels displayed a significant capability in improving PFS. Moreover, persistent tumor residual lesions, positive HBV DNA and hepatitis B flares might be causes of tumor progression in these patients.

Reusable and Efficient Polystryrene-supported Acidic Ionic Liquid Catalyst for Mononitration of Aromatic Compounds

Li, Li Xia,Ling, Qi Long,Liu, Zu Liang,Xing, Xiao Dong,Zhu, Xiao Qin,Meng, Xiao Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.10

A series of polystyrene-supported 1-(propyl-3-sulfonate)-3-methyl-imidazolium hydrosulfate acidic ionic liquid (PS-$[SO_3H-PMIM][HSO_4]$) catalysts were prepared and tested for mononitration of simple aromatics compounds with nitric acid. It was found that the reactivity of the catalysts increased with increasing $[SO_3H-PMIM][HSO_4]$ content. The para-selectivity was not only related to the $[SO_3H-PMIM][HSO_4]$ content but also the substituent groups in aromatics. A reaction mechanism of nitration over this new catalyst was proposed. The catalytic activity of this catalyst decreased slightly after fifth runs in the synthesis of nitrotoluene.

Four new sesqui-lignans isolated from Acanthopanax senticosus and their diacylglycerol acyltransferase (DGAT) inhibitory activity

Li, J.L.,Li, N.,Lee, H.S.,Xing, S.S.,Qi, S.Z.,Tuo, Z.D.,Zhang, L.,Li, B.B.,Chen, J.G.,Cui, L. Elsevier 2016 Fitoterapia Vol.109 No.-

<P>Four new sesqui-lignans, (7R, 7'R, 7 '' S, 8S, 8'S, 8 '' S)-4',5 ''-dihydroxy-3,5,3',4 ''-tetramethoxy-7,9':7',9-diepoxy4,8 ''-oxy-8,8'-sesquineo-lignan-7',9 ''-diol (1), (7R, 7'R, 7 '' S, 8S, 8'S, 8 '' S)-4',3'-dihydroxy-3,5,3',5',4'pentamethoxy-7,9':7',9-diepoxy-4,8'-oxy-8,8'-sesquineo-lignan-7',9'-diol (2), (7R, 7'R, 7'S, 8S, 8'S, 8'S)-3',4'dihydroxy-3,5,4',5 ''-tetramethoxy-7,9':7',9-diepoxy-4,8'-oxy-8,8'-sesquineo-lignan-7',9'-diol (3) and acanthopanax A (7) together with three known compounds (4-6) were isolated from the EtOAc-soluble extract of Acanthopanax senticosus. Their structures were elucidated on the basis of spectroscopic and physicochemical analyses. All the isolates were evaluated for in vitro inhibitory activity against DGAT1 and DGAT2. Among them, compounds 1-6 were found to exhibit selective inhibitory activity on DGAT1 with IC50 values ranging from 61.1 1.3 to 97.7 1.1 111\4 and compound 7 showed selective inhibition of DGAT2 with IC50 value 93.2 1.2. (C) 2016 Elsevier B.V. All rights reserved.</P>

Effects of Supplementing Different Combinations of Nitrogen Supplements on Digestibility and Performance in Sheep Fed Wheat Straw Diets

Li, Yong,Li, Defa,Xing, Jianjun,Li, Shengli,Han, Yang,Han, In K. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.10

Thirty-six (20 wethers and 16 rams) adult Gansu highland finewool sheep with an average liveweight of $19.42{\pm}1.83kg$ were used. The sheep were allocated to 4 treatments and confined in individual feeding stalls. The 80 day feeding trial was divided into 2 periods of 38 and 42 days. The sheep had access to clean water and were fed chopped wheat straw ad libitum. Daily supplemented diets of 150 g for the first period and 200 g for the later period were provided. The 4 types of supplements were: Wheat bran type (I), which is the traditional feeding practice in the area and the control group; Linseed cake type (II); Linseed cake+poultry litter type (III); Poultry litter+distiller grains type (IV). The digestibility trial was conducted using a total collection method at the end of the first period in the feeding trial. The results showed that the digestibilities of DM, OM, CP, CF, NDF and ADF in group II and III were increased to more than 50%. CP and NDF digestibility in group II were significantly increased by 23.6% and 25.5% respectively over group I (p<0.01) and by 10.1% and 13.1% respectively over group III (p<0.05). The digestibility of DM, OM, CP, CF, NDF and ADF in group IV was lower than group I by 4.4 to 8.4% (p>0.05). Compared to group I, group II and III increased straw intake by 17.6% (p<0.01) and 10.8% (p<0.05). The ADG increased from 16.2 g in group I to 45.3 g and 32.8 g (p<0.01) in group II and III respectively. The ratio of supplement intake to gain decreased from 10.7 in group I to 3.9 and 5.4 (p<0.01) in group II and III respectively. The ratio of supplement cost to gain also decreased by 60.0% and 64.3%, respectively. The wool growth rate and wool strength in these 2 groups were noticeably improved. This significantly enhanced the wool's textile value. The above parameters in group IV were lower than in group I, except the ratio of supplement cost to gain. This research indicates that local low quality nitrogen resources could be used effectively and economically to improve the utilization of wheat straw fed to sheep on smallholder farms under harsh loess plateau conditions in the winter.

Dexmedetomidine targets miR-146a and participates in the progress of chronic obstructive pulmonary disease in vivo and in vitro

Li Na,Li Shuangfeng,Wu Yehua,Xiong Lu,Li Tiejun,Xing Dandan,Li Qiuchang,Wu Duozhi 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Chronic obstructive pulmonary disease (COPD) is a chronic lung disease and the third leading cause of death in the world. Dexmedetomidine has been reported to efectively inhibit histamine-induced bronchoconstriction. However, the molecular mechanism of dexmedetomidine in COPD has not been found. Objective To explore the role and mechanism of dexmedetomidine in COPD, and to provide theoretical basis for clinical treatment of COPD. Methods The expression of miR-146a was regulated by mimics or inhibitor and the relative expression of apoptotic proteins p53, Bax and Bcl-2 in human bronchial epithelial 16HBE cells was determined by real-time PCR and Western blot. Dexmedetomidine was treated for 16HBE cells and alveolar epithelial type II cells (AEC2), the cell apoptosis was detected by TUNEL and Hoechst33342 staining. A COPD rat model was established by smoking to test the efects of dexmedetomidine on the progression of COPD. The levels of IL-6, IL-1β and TNF-α in serum were measured by ELISA and the protein concentration of bronchoalveolar lavage fuid (BALF) was also detected in dexmedetomidine treated COPD rat model. Results miR-146a promoted 16HBE cell apoptosis and reduced cell proliferation. Additionally, dexmedetomidine was showed to reduce the 16HBEL cell apoptosis through reducing the expression of miR-146a. Moreover, dexmedetomidine regulated cell apoptosis and cell apoptosis through miR-146a in AEC2 cells. More importantly, dexmedetomidine attenuated the morphology and pathology of COPD rat model. Conclusion Dexmedetomidine reduced 16HBE cells and AEC2 cell apoptosis and attenuated COPD by down-regulating miR-146a.