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Kim, Shin Ae,Byun, Kyung Min,Kim, Kyujung,Jang, Sung Min,Ma, Kyungjae,Oh, Youngjin,Kim, Donghyun,Kim, Sung Guk,Shuler, Michael L,Kim, Sung June IOP Pub 2010 Nanotechnology Vol.21 No.35
<P>We demonstrated enhanced localized surface plasmon resonance (SPR) biosensing based on subwavelength gold nanoarrays built on a thin gold film. Arrays of nanogratings (1D) and nanoholes (2D) with a period of 200 nm were fabricated by electron-beam lithography and used for the detection of avian influenza DNA hybridization. Experimental results showed that both nanoarrays provided significant sensitivity improvement and, especially, 1D nanogratings exhibited higher SPR signal amplification compared with 2D nanohole arrays. The sensitivity enhancement is associated with changes in surface-limited reaction area and strong interactions between bound molecules and localized plasmon fields. Our approach is expected to improve both the sensitivity and sensing resolution and can be applicable to label-free detection of DNA without amplification by polymerase chain reaction. </P>
Kim, Kyujung,Kim, Dong Jun,Moon, Seyoung,Kim, Donghyun,Byun, Kyung Min IOP Pub 2009 Nanotechnology Vol.20 No.31
<P>Enhanced detection of multiple targets such as self-assembled monolayer (SAM) formation, DNA hybridization, and ethanol ambient changes was explored using localized surface plasmon resonance (LSPR) excited by metallic surface nanogratings. The sensitivity enhancement depends on the target as well as the nanostructure with a maximum at 242% over a conventional structure when detecting an 11-mercaptoundecanoic acid SAM with an LSPR structure of 200 nm period. The measured enhancement shows smaller target-dependent variance when detecting various layered biointeractions, while structure-dependent variance was much larger. The result suggests the feasibility of the efficient detection of multiple biointeractions at enhanced sensitivity and extends the applicability of a nanostructured LSPR biosensor for diverse biomolecular events. </P>
Kim, Kyujung,Kim, Dong Jun,Cho, Eun-Jin,Suh, Jin-Suck,Huh, Yong-Min,Kim, Donghyun IOP Pub 2009 Nanotechnology Vol.20 No.1
<P>We investigated evanescent field enhancement based on subwavelength nanogratings for improved sensitivity in total internal reflection microscopy of live cells. The field enhancement is associated with subwavelength-grating-coupled plasmon excitation. An optimum sample employed a silver grating on a silver film and an SF10 glass substrate. Field intensity was enhanced by approximately 90% when measured by fluorescent excitation of microbeads relative to that on a bare prism as a control, which is in good agreement with numerical results. The subwavelength-grating-mediated field enhancement was also applied to live cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively. </P>
Development of Molecular Markers for Xanthomonas axonopodis Resistance in Soybean
Kim Ki-Seung,Van Kyujung,Kim Moon Young,Lee Suk-Ha The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.5
A single recessive gene, rxp, controls the bacterial leaf pustule (BLP) resistance in soybean and in our previous article, it has been mapped on linkage group (LG) D2 of molecular genetic map of soybean. A total of 130 recombinant inbred lines (RILs) from a cross between BLP-resistant SS2-2 and BLP-susceptible Jangyeobkong were used to identify molecular markers linked to rxp. Fifteen simple sequence repeat (SSR) markers on LG D2 were screened to construct a genetic map of rxp locus. Only four SSR markers, Satt135, Satt372, Satt448, and Satt486, showed parental polymorphisms. Using these markers, genetic scaffold map was constructed covering 26.2cM. Based on the single analysis of variance, Satt372 among these four SSR markers was the most significantly associated with the resistance to BLP. To develop new amplified fragment length polymorphism (AFLP) marker linked to the resistance gene, bulked segregant analysis (BSA) was employed. Resistance and susceptible bulks were made by pooling equal amount of genomic DNAs from ten of each in the segregating population. A total of 192 primer combinations were used to identify specific bands to the resistance, selecting three putative AFLP markers. These AFLP markers produced the fragment present in SS2-2 and the resistant bulk, and not in Jangyeobkong and the susceptible bulk. Linkage analysis revealed that McctEact97 $(P=0.0004,\;R^2=14.67\%)$ was more significant than Satt372, previously reported as the most closely linked marker.
Kim, Kyujung,Oh, Youngjin,Lee, Wonju,Kim, Donghyun Optical Society of America 2010 Optics letters Vol.35 No.20
<P>In this Letter, we explore plasmonics-based spatially activated light microscopy (PSALM) for sub-diffraction-limited imaging of biomolecules. PSALM is based on the spatially switched activation of local amplified electromagnetic hot spots under multiple light incidence conditions. The hot spots are associated with surface plasmons that are excited and localized by surface nanostructures. The feasibility of the concept was demonstrated by imaging fluorescent nanobeads on a two-dimensional gold nanograting of a 100-nm-wide grating ridge, the size of which is the measure of the imaging resolution. The result confirms the performance of PSALM for imaging nanobeads at a resolution below the conventional diffraction limit.</P>
Kim, Kyujung,Cho, Eun-Jin,Huh, Yong-Min,Kim, Donghyun Optical Society of America 2007 Optics letters Vol.32 No.21
<P>We investigated experimentally the evanescent field enhancement based on dielectric thin films in total internal reflection microscopy. The sample employed two layers of Al2O3 and SiO2 deposited on an SF10 glass substrate. Field intensity enhancement measured by fluorescent excitation of microbeads relative to that of a control sample without dielectric films was polarization dependent, determined as 4.2 and 2.4 for TE and TM polarizations, respectively, and was in good agreement with numerical results. The thin-film-based field enhancement was also applied to live-cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.</P>
Parametric Studies on Artificial<i>Morpho</i>Butterfly Wing Scales for Optical Device Applications
Kim, Hyun Myung,Kim, Sang Hyeok,Lee, Gil Ju,Kim, Kyujung,Song, Young Min Hindawi Limited 2015 Journal of nanomaterials Vol.2015 No.-
<P>We calculated diffraction efficiencies of grating structures inspired by<I>Morpho</I>butterfly wings by using a rigorous coupled-wave analysis method. The geometrical effects, such as grating width, period, thickness, and material index, were investigated in order to obtain better optical performance. Closely packed grating structures with an optimized membrane thickness show vivid reflected colors and provide high sensitivity to surrounding media variations, which is applicable to vapor sensing or healthcare indicators.<I>Morpho</I>structures with high index materials such as zinc sulfide or gallium phosphide generate white color caused by broadband reflection that can be used as reflected light sources for display applications.</P>