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      저자 : Kleinegger, Cynthia L. (검색결과 1 건)
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        Development of an In Vitro Candida albicans Oral Infection Model

        Lund, Trace,Kleinegger, Cynthia L.,Wertz, Philip W.,Drake, David R. Korean Academy of Oral Biology and the UCLA Dental 1997 International Journal of Oral Biology Vol.22 No.4

        Objectives: The purpose of the present study was to develop an in vitro model for studies of Candida albicans oral infections. Design : Sheets of porcine buccal epithelium were cut at a thickness of 300 μm and glued with cyanoacrylate to a plastic backing. Circular 1-cm-diameter disks of the plastic-backed epithelium were cut, and incubated with Candida albicans. At various incubation times, numbers and appearance of Candida albicans on the tissue disks were assessed. Materials : Porcine tissue was obtained at a slaughterhouse. Candida selective Chromagar plates were purchased from Hardy Diagnostics of Santa Maria, CA. Other materials were from Sigma Chemical Company, St. Louis, Mo. Methods : Candida albicans was cultured in Sabouraud Dextrose Broth at 37℃, overnight. Tissue disks were incubated with standardized suspensions. At time of harvest, some disks were fixed for examination by scanning electron microscopy, while others were homogenized and spiral-plated onto Candida-selective agar. Results : At three hours, there were 2.1×10 exp (5) adherent yeast per disk. By 24 hours of incubation, there was noticeable hyphae formation, and by 48 hours hyphae predominated and tissue invasion was evident. Conclusions : The porcine buccal epithelial disk provides an in vitro model for oral Candida albicans infections in which adherence, growth,, and the bud to hyphae transition and tissue invasion process may be investigated.

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