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RISS 인기검색어
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- 저자 : Kim Kyumson (검색결과 3 건)
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Kim, Kyumson,Kim, Kwang-Pyo,Choi, Jeongjoon,Lim, Jeong-A,Lee, Junghyun,Hwang, Sunyoung,Ryu, Sangryeol American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.15
<B>ABSTRACT</B><P><I>Cronobacter sakazakii</I> is an opportunistic pathogen that actively invades host eukaryotic cells. To identify invasion factors responsible for the intestinal translocation of <I>C. sakazakii</I>, we constructed for the first time outer membrane protein X (OmpX) and A (OmpA) deletion mutants using the lambda Red recombination system. The <I>ompX</I> and <I>ompA</I> deletion mutants showed significantly reduced invasion of human enterocyte-like epithelial Caco-2 and human intestinal epithelial INT-407 cells, and significantly fewer mutant cells were recovered from the livers and spleens of rat pups. Furthermore, compared with intact target cells, the invasion and initial association potentials of the mutants increased at a rate similar to that of the wild type in tight-junction-disrupted target cells, suggesting that OmpX and OmpA are involved in basolateral invasion by <I>C. sakazakii</I>. This is the first report of <I>C. sakazakii</I> virulence determinants that are essential for basolateral invasion and that may be critical for the virulence of <I>C. sakazakii</I>.</P>
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Chandrapala, Dilini,Kim, Kyumson,Choi, Younho,Senevirathne, Amal,Kang, Dong-Hyun,Ryu, Sangryeol,Kim, Kwang-Pyo American Society for Microbiology 2014 Infection and immunity Vol.82 No.5
<P><I>Cronobacter sakazakii</I> is an opportunistic pathogen that causes neonatal meningitis and necrotizing enterocolitis. Its interaction with intestinal epithelium is important in the pathogenesis of enteric infections. In this study, we investigated the involvement of the <I>inv</I> gene in the virulence of <I>C. sakazakii</I> ATCC 29544 <I>in vitro</I> and <I>in vivo</I>. Sequence analysis of <I>C. sakazakii</I> ATCC 29544 <I>inv</I> revealed that it is different from other <I>C. sakazakii</I> isolates. In various cell culture models, an Δ<I>inv</I> deletion mutant showed significantly lowered invasion efficiency, which was restored upon genetic complementation. Studying invasion potentials using tight-junction-disrupted Caco-2 cells suggested that the <I>inv</I> gene product mediates basolateral invasion of <I>C. sakazakii</I> ATCC 29544. In addition, comparison of invasion potentials of double mutant (Δ<I>ompA Δinv</I>) and single mutants (Δ<I>ompA</I> and Δ<I>inv</I>) provided evidence for an additive effect of the two putative outer membrane proteins. Finally, the importance of <I>inv</I> and the additive effect of putative Inv and OmpA were also proven in an <I>in vivo</I> rat pup model. This report is the first to demonstrate two proteins working synergistically <I>in vitro</I>, as well as <I>in vivo</I> in <I>C. sakazakii</I> pathogenesis.</P>
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Prevalence and Genetic Diversity of Bacillus cereus in Dried Red Pepper in Korea
CHOO, EUIYOUNG,JANG, SUNG SIK,KIM, KYUMSON,LEE, KWANG-GEUN,HEU, SUNGGI,RYU, SANGRYEOL International Association for Food Protection 2007 Journal of food protection Vol.70 No.4
<P>Bacillus cereus is a foodborne spore-forming bacterial pathogen that is ubiquitous in the natural environment. Infections with this pathogen manifest as diarrheal or emetic types of food poisoning. In this study, 140 samples of dried red pepper purchased in Korea were assayed for the presence of B. cereus according to the U.S. Food and Drug Administration standard culture method. A multiplex PCR assay was developed for the rapid confirmation of B. cereus as an alternative to conventional biochemical confirmation tests. The genetic diversity of B. cereus isolates was investigated using a random amplified polymorphic DNA (RAPD) assay. B. cereus was found in 84.3% of the dried red pepper samples, with an average concentration of 1.9 × 104 CFU/g. B. cereus could be detected and distinguished from B. thuringiensis in the multiplex PCR assay by using the BCFW1 plus BCrevnew and the K3 plus K5 primer sets designed to detect the gyrB gene of B. cereus and B. thuringiensis and the cry gene of B. thuringiensis. A RAPD assay using the OPG 16 and MUP 3 primers was used to successfully distinguish among isolates, thus elucidating the genetic diversity of B. cereus isolates. The discriminating ability of the OPG 16 primer (142 types) was about threefold higher than that of MUP 3 (52 types) in the RAPD assay.</P>
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