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Kihyuck Choi,Geun Ju Son,Shabir Ahmad,Seung Yeup Lee,Hyoung Ju Lee,Seon-Woo Lee 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.4
Bacterial traits for virulence of Ralstonia solanacearum causing lethal wilt in plants were extensively studied but are not yet fully understood. Other than the known virulence factors of Ralstonia solanacearum, this study aimed to identify the novel gene(s) contributing to bacterial virulence of R. solanacearum. Among the transposon-inserted mutants that were previously generated, we selected mutant SL341F12 strain produced exopolysaccharide equivalent to wild type strain but showed reduced virulence compared to wild type. In this mutant, a transposon was found to disrupt the murI gene encoding glutamate racemase which converts L-glutamate to D-glutamate. SL341F12 lost its motility, and its virulence in the tomato plant was markedly diminished compared to that of the wild type. The altered phenotypes of SL341F12 were restored by introducing a full-length murI gene. The expression of genes required for flagella assembly was significantly reduced in SL341F12 compared to that of the wild type or complemented strain, indicating that the loss of bacterial motility in the mutant was due to reduced flagella assembly. A dramatic reduction of the mutant population compared to its wild type was apparent in planta (i.e., root) than its wild type but not in soil and rhizosphere. This may contribute to the impaired virulence in the mutant strain. Accordingly, we concluded that murI in R. solanacearum may be involved in controlling flagella assembly and consequently, the mutation affects bacterial motility and virulence.
Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere
Roy, Nazish,Choi, Kihyuck,Khan, Raees,Lee, Seon-Woo The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.4
Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato root exudates. Bacterial cells were harvested to amplify V3 and V4 regions of 16S rRNA gene for QIIME based sequence analysis and were also treated to Hawaii 7996 prior to Ralstonia solanacearum inoculation. The disease progress indicated that the upland MM9 $1^{st}$ transfer suppressed the bacterial wilt. Community analysis revealed that species richness was declined by successive cultivation of the MF. The upland MM9 $1^{st}$ transfer harbored population of phylum Proteobacteria (98.12%), Bacteriodetes (0.69%), Firmicutes (0.51%), Actinobacteria (0.08%), unidentified (0.54%), Cyanobacteria (0.01%), FBP (0.001%), OD1 (0.001%), Acidobacteria (0.005%). The family Enterobacteriaceae of Proteobacteria was the dominant member (86.76%) of the total population of which genus Enterobacter composed 86.76% making it a potential candidate to suppress bacterial wilt. The results suggest that this mixed culture approach is feasible to harvest microorganisms which may function as biocontrol agents.
Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere
Nazish Roy,Kihyuck Choi,Raees Khan,Seon-Woo Lee 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4
Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato root exudates. Bacterial cells were harvested to amplify V3 and V4 regions of 16S rRNA gene for QIIME based sequence analysis and were also treated to Hawaii 7996 prior to Ralstonia solanacearum inoculation. The disease progress indicated that the upland MM9 1st transfer suppressed the bacterial wilt. Community analysis revealed that species richness was declined by successive cultivation of the MF. The upland MM9 1st transfer harbored population of phylum Proteobacteria (98.12%), Bacteriodetes (0.69%), Firmicutes (0.51%), Actinobacteria (0.08%), unidentified (0.54%), Cyanobacteria (0.01%), FBP (0.001%), OD1 (0.001%), Acidobacteria (0.005%). The family Enterobacteriaceae of Proteobacteria was the dominant member (86.76%) of the total population of which genus Enterobacter composed 86.76% making it a potential candidate to suppress bacterial wilt. The results suggest that this mixed culture approach is feasible to harvest microorganisms which may function as biocontrol agents.
김남성(Nam Seong KIM),김기혁(Kihyuck KIM),정영대(Youngdae CHUNG),최창기(Changgi CHOI),이도경(Dohkyeong LEE),이승재(Seungjae LEE),성기석(Geesuk Sung),전영민(Youngmin JHON),성하민(Hamin SUNG),오경환(Kyunghwan OH),김종기(Jongki KIM),박지영 한국생산제조학회 2010 한국생산제조시스템학회 학술발표대회 논문집 Vol.2010 No.10
기존 레이저 미세가공기술 한계를 극복하여 LCD/AMOLED 등의 가공물 내부와 표면에 10㎛ ~ 500㎚ 까지의 초정밀청정가공을 하는 기술개발로서 관련된 고부가가치 생산시스템개발 및 시장요구형 50W/300fs/1035㎚/10㎒ 펨토초 광섬유레이저 기술개발을 소개한다. 기술개발은 2010년6월부터 2015년3월말까지 진행할 예정이며 현재 3개월여의 기술개발을 수행하고 있다. 1차년도 기술개발목표는 레이저광원으로서 20㎽/1035㎚/10㎒/300fs의 모드잠금광섬유레이저를 개발하는 것과 응용프로세스개발은 7㎛급의 내부 3차원 초미세 가공과 표면 초미세가공 등이다. (지식경제부 산업원천기술개발사업 프로젝트번호 10037371).
Rhizosphere microbiome structure alters to enable wilt resistance in tomato
Kwak, Min-Jung,Kong, Hyun Gi,Choi, Kihyuck,Kwon, Soon-Kyeong,Song, Ju Yeon,Lee, Jidam,Lee, Pyeong An,Choi, Soo Yeon,Seo, Minseok,Lee, Hyoung Ju,Jung, Eun Joo,Park, Hyein,Roy, Nazish,Kim, Heebal,Lee, M Nature Pub. Co 2018 Nature biotechnology Vol.36 No.11
<P> Tomato variety Hawaii 7996 is resistant to the soil-borne pathogen Ralstonia solanacearum, whereas the Moneymaker variety is susceptible to the pathogen. To evaluate whether plant-associated microorganisms have a role in disease resistance, we analyzed the rhizosphere microbiomes of both varieties in a mesocosm experiment. Microbiome structures differed between the two cultivars. Transplantation of rhizosphere microbiota from resistant plants suppressed disease symptoms in susceptible plants. Comparative analyses of rhizosphere metagenomes from resistant and susceptible plants enabled the identification and assembly of a flavobacterial genome that was far more abundant in the resistant plant rhizosphere microbiome than in that of the susceptible plant. We cultivated this flavobacterium, named TRM1, and found that it could suppress R. solanacearum-disease development in a susceptible plant in pot experiments. Our findings reveal a role for native microbiota in protecting plants from microbial pathogens, and our approach charts a path toward the development of probiotics to ameliorate plant diseases. </P>
김남성(Nam Seong KIM),김기혁(Kihyuck KIM),정영대(Youngdae CHUNG),최창기(Changgi CHOI),이도경(Dohkyeong LEE),이승재(Seungjae LEE),성기석(Geesuk Sung),전영민(Youngmin JHON),성하민(Hamin SUNG),오경환(Kyunghwan OH),김종기(Jongki KIM),박지영 한국생산제조학회 2010 한국공작기계학회 추계학술대회논문집 Vol.2010 No.-
기존 레이저 미세가공기술 한계를 극복하여 LCD/AMOLED 등의 가공물 내부와 표면에 10㎛ ~ 500㎚ 까지의 초정밀청정가공을 하는 기술개발로서 관련된 고부가가치 생산시스템개발 및 시장요구형 50W/300fs/1035㎚/10㎒ 펨토초 광섬유레이저 기술개발을 소개한다. 기술개발은 2010년6월부터 2015년3월말까지 진행할 예정이며 현재 3개월여의 기술개발을 수행하고 있다. 1차년도 기술개발목표는 레이저광원으로서 20㎽/1035㎚/10㎒/300fs의 모드잠금광섬유레이저를 개발하는 것과 응용프로세스개발은 7㎛급의 내부 3차원 초미세 가공과 표면 초미세가공 등이다. (지식경제부 산업원천기술개발사업 프로젝트번호 10037371).