RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • Membrane Microdomains as a Platform of Carbohydrate-mediated Cell-cell Interactions during Gastrulation

        Ken Kitajima 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

        It has been shown that membrane microdomains (or rafts) are important as a hot spot for signal transduction. However, little attention has been paid to the fact that glycan chains are enriched in the microdomains. We have recently proposed a hypothesis that the microdomains are involved in the protein- and glycan-mediated cell adhesion and the subsequent signal transduction, and have demonstrated it in sperm-egg interactions at fertilization of sea urchin [1-3] To extend this hypothesis in early embryogenesis, we have studied on microdomains in developing embryos of medaka fish, Oryziaslatipes. Here wes how several lines of evidence supporting our hypothesis: (i) The micro domains prepared from early gastrula embryos were rich in cholesterol and sphingomyelin, and contained E-cadherin and LeX-glycan-containing glycoproteins (LeX-gp) and glycolipids (LeX-gsl), together with transducer proteins like c-Srckinase. The LeX-glycans and E-cadherin are known to be involved in morula compaction (tight cell adhesion) in mouse; (ii) Disruption of the microdomains of early gastrula embryos by treatment with methyl-b-cyclodextrin (MBCD) or C2-ceramide, impaired epiboly, the first cell migration event that occurs during gastrulation process. The most typical phenotype of the microdomain-disrupted embryo was detachment of blastodermal cells. Interestingly, the MBCD-induced disruption of microdomains and the epiboly impairment were restored when cholesterol was added back to the embryos. Thus, microdomain formation is critical in epiboly [4]; (iii) The isolated microdomains could bind to the microdomains in the presence of Ca2+ in LeX-glycan- and E-cadherin-dependent manners. These results suggest that the microdomain formation is important in the protein- and carbohydrate-mediated cell-cell interactions.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼