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Shengxiang Zhang,Yuanyuan Shi,Chunmiao Shan,Liqiang Zhao,Kelong Ma,Luqi Huang,Jiawen Wu 한국식물학회 2020 Journal of Plant Biology Vol.63 No.3
Polygonatum odoratum (Mill.) Druce (P. odoratum) is a traditional Chinese herb. The isofavonoids of P. odoratum are important medicinal bioactive compounds for the treatment of hypoimmunity, rheumatic heart disease, cardiovascular diseases, and diabetes. This study used RNA sequencing to identify potential genes regulating isofavonoid biosynthesis in P. odoratum. To do this, we generated an overview of metabolic and secondary metabolic pathways, using MAPMAN, and identifed 96 genes encoding seven key enzymes involved in isofavonoid biosynthesis through their KEGG annotation. Alignment of phenylalanine ammonia-lyase or chalcone synthase amino acid sequences revealed well-conserved sequences, spatial structures, and active sites. We also verifed the expression of key genes encoding phenylalanine ammonia-lyase and chalcone synthase by quantitative real-time PCR (qRT-PCR). This analysis of the isofavonoid biosynthesis pathway and its crucial enzymes in P. odoratum lays the foundation for uncovering the regulatory mechanism of isofavonoid biosynthesis.
Jinlan Gou,Kelong Zhang,Yuansheng Lin,Yong Li,Can Ma,Hanbing Ke 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.8
The compressor is one of the key components in the closed supercritical carbon dioxide (S-CO 2 ) Brayton cycle, but its design method is far from mature. It is naturally expected that the well-established design method of the air compressor can provide favorable guidelines, on the basis of further understanding the effects of the physical property on the compressor flow field. Considering that isentropic compression is one of the core physical processes in the compressor, the physical property effects on this process were mainly investigated in this work. Similarity criterion was considered, and the change rate discrepancy of the main variables in this process between S-CO 2 and the ideal air was fully analyzed. Results show that S-CO 2 is compressed faster than the ideal air in most cases, along with generating smaller Mach number and larger pressure rise ratio. It is noted the important parameter of the static pressure coefficient distribution with S-CO 2 in the compression process is almost the same as that with the ideal air at low Mach number, which is conductive to the extension of the air compressor research experience, but it is quite different at high Mach number. The simulation cases about compressor cascade are further applied and prove the suitability of the revealed physical property effects in the compressor passage. Understanding these effects on the compression process is helpful to improve the design method of the S-CO 2 compressor.