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Wang, Huan,Chung, Pil Joong,Liu, Jun,Jang, In-Cheol,Kean, Michelle J.,Xu, Jun,Chua, Nam-Hai Cold Spring Harbor Laboratory Press 2014 Genome Research Vol.24 No.3
<P>Recent research on long noncoding RNAs (lncRNAs) has expanded our understanding of gene transcription regulation and the generation of cellular complexity. Depending on their genomic origins, lncRNAs can be transcribed from intergenic or intragenic regions or from introns of protein-coding genes. We have recently reported more than 6000 intergenic lncRNAs in <I>Arabidopsis</I>. Here, we systematically identified long noncoding natural antisense transcripts (lncNATs), defined as lncRNAs transcribed from the opposite DNA strand of coding or noncoding genes. We found a total of 37,238 sense–antisense transcript pairs and 70% of annotated mRNAs to be associated with antisense transcripts in <I>Arabidopsis</I>. These lncNATs could be reproducibly detected by different technical platforms, including strand-specific tiling arrays, Agilent custom expression arrays, strand-specific RNA-seq, and qRT-PCR experiments. Moreover, we investigated the expression profiles of sense–antisense pairs in response to light and observed spatial and developmental-specific light effects on 626 concordant and 766 discordant NAT pairs. Genes for a large number of the light-responsive NAT pairs are associated with histone modification peaks, and histone acetylation is dynamically correlated with light-responsive expression changes of NATs.</P>
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Surface functionalized carbon microspheres for the recovery of copper ion from refinery wastewater
Subrata Mondal,Addisu Tadesse Derebe,Kean Wang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.1
Micro-sized carbon spheres (CNS) were synthesized by hydrothermal process from glucose solution at 443.15 K and two different reaction times. The synthesized CNS samples were surface functionalized with hydroxyl (-OH) functional groups using NaOH treatment, and were tested in batch adsorption to remove heavy metal ion (Cu2+) from aqueous solutions. Experimental results revealed that CNS contains mostly amorphous carbon. NaOH functionalized CNS had significantly higher adsorption capacity of copper ~170×10−3 Kg Cu2+/Kg-CNS as compared to the untreated CNS. The adsorption isotherms were well fitted by the Langmuir isotherm equation. The surface morphology of the native and functionalized CNS samples was characterized by a number of techniques and based on which the adsorption of copper ion was discussed.
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