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Patel, Kamlesh,Negi, P S,Swarup, Ram,Kim, Jeong Hwan,Kang, Jin-Seob,Chow, Michael W K,Tsui, C M,Dressler, Erik,Shay, Wen-Tron,Li, Hua,Shan, Yueyan Springer-Verlag 2012 METROLOGIA -BERLIN- Vol.49 No.-
<P>A measurement comparison of complex reflection coefficient has been carried out among six national metrology laboratories in coaxial system at frequencies of 50 MHz, 2 GHz, 10 GHz and 18 GHz. Three 50 Ω coaxial mismatches (voltage standing wave ratios 1.2, 1.5 and 2.0) in 7 mm connectors have been measured for their complex reflection coefficients in the magnitude and phase form. The six laboratories participated are NPL India, KRISS Korea, SCL Hong Kong, CMS ITRI Taiwan, NMC Singapore and CSIR-NML South Africa (now NMISA). The National Physical Laboratory (India) acted as the pilot laboratory for the comparison. Good agreement between the values provided by the participating laboratories has been observed: the degrees of equivalence for all the values reported, with respect to the reference value and between pairs of laboratories, are less than their associated uncertainties, at a level of confidence of 95%.</P><P>Main text.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.</P><P>The final report has been peer-reviewed and approved for publication by APMP, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>
Optimization of Glucoamylase Production by Colletotrichum sp. KCP1 Using Statistical Methodology
Vimal S. Prajapati,Ujjval B. Trivedi,Kamlesh C. Patel 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.1
Glucoamylase is a key enzyme used in the food processing as well as in commercial production of glucose from starch. A natural fungal strain identified as Colletotrichum sp. KCP1 using 18S rDNA partial genome sequencing has been studied for optimization of glucoamylase production. Media components were screened and optimized through the statistical approach for the synthesis of glucoamylase in solid state fermentation using wheat bran as the substrate. The medium components influencing the enzyme production were identified using Plackett-Burman design. Among various variables screened along with wheat bran as major growth substrate, starch, whey, and casein acid hydrolyasate were found to be most significant. The optimum concentrations of these significant parameters were determined employing the response surface central composite design, revealing starch concentration (1.5 g),whey (0.1 mL), and casein acid hydrolysate (0.1 g) per 5 g of wheat bran for highest enzyme production.