당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

서독의 통일외교 : 한국내 논의제기를 위한 시론

길정우 고려대학교 一民국제관계연구원 1996 국제관계연구 Vol.1 No.2

인슐린 주사제의 고충실 액체색층기 분석

우정택,팽정렬,김성운,양인명,김진우,김영설,김광원,최영길 최신의학사 1993 最新醫學 Vol.36 No.8

좌측 상하악 구치 후방부에 발생한 다형성 선종의 치험례

우길상,이은진,김상규,김진수,변기정 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Pleomorphic adenoma is a circumscribed tumor characterized microscopically by its pleomorphic or mixed appearance, and its clearly recognizable epithelial tissue intermingled with areas of mucoid, myxoid, or chondroid appearance. Plemorphic adenoma, the most common tumor of the salivary glands, is now generally accepted as epithelial and benign adenoma. The palate is the most common site for minor salivary gland tumor. We experienced a case of pleomorphic adenoma occuring in the left retromolar pad area of unusual location. It is unusually large size so that the patient cannot close his mouth. The tumor occupied his mouth fully so that he has much difficulty in closing his mouth and breathing his breath. The lesion was treated with complete excision and reconstruced with buccal fat pedicle flap immediately on the operation under general anesthesia. Histopathological finding indicated pleomorphic adenoma on the left retromolar pad area.

견인용 동기전동기의 시동 및 운전특성 해석에 관한 연구

황정원,조용길,송호신,박한규,우정인 東亞大學校 1994 東亞論叢 Vol.31 No.1

It needs a large capacity of equipments to test the starting performance of middle and large synchronous motors in a factory. This paper propose a new method which is the combination of single phase AC applied test and usual routine test with a small synchronous test machine and testing equipments. We can obtain the results from these proposed tests as follows ; (1) The starting performance and stabilized operation at variable speed is predicted from this results. (2) The armature winding leakage reactance is resulted from the no-load saturation curve, the short-circuit charactristic curve and the armature-reaction magnetomotive force converted to the field current. (3) This test must be performed twice, one for short-circuiting the field winding, the other for joining a resistance between the field terminals, as a result the unknown constants in equivalent circuits are identified. (4) Inverter-fed three phase voltage with variable frequency is applied to armature terminals, as a result, We show the actual armature leakage reactance and reaction of the induced harmonic voltages from rotor.

전자선 증착장치 및 ITO 박막의 제작과 그 특성에 관한 연구

김수길,최범식,우정주,고년규,황정남,정원모,이철주 연세대학교 자연과학연구소 1982 學術論文集 Vol.9 No.-

태양 전지의 제작시 투명 전극막으로 쓰이는 ITO 박막을 제조하기 위하여 cathode 접지 방식의 전자총을 가진 정전 편향 집속형의 전자선 증착 장치를 제작하였다. 가속전압이 0∼15kV, 필라멘트 전류가 0∼28A, 두 전극판 사이의 간격이 11mm, anode와 deflector 사이의 간격이 30mm인 최적 실험조건하에서 생성된 전자선 spot의 크기는 길이가 20mm 폭이 1mm였으며, 전자선의 출력은 약 400W였다. 이 전자선 증착 장치를 이용하여 1600℃까지의 증발 온도를 가진 도체와 절연체 등을 쉽게 증착시킬 수 있었으며, 만드어진 ITO 박막은 약 5000Å의 두께에서 10^-1∼10^-2Ω·㎝의 비저항과 95%의 광투과율을 갖는다. An electron beam evaporator with a cathode grounded type electron gun was designed and constructed in order to deposit ITO thin films for photovoltaic applications. The optimum condition was observed with electode spacing of 11mm, 30mm between anode and deflector, a cathode inner diameter of 3mm and an anode inner diameter of 5mm. As the accelerating voltage varies from 0 to 15kV and ghe filament current from 0 to 28 A, the minimum electron beam spot has a 20mm length, and 1mm width and the output power ranges from 0 to 400W under the above optimum conditions. We can deposit materials whose evaporation temperature is over 1600℃ using this evaporator. And as a result ITO thin film showed a low resistivity of 10^-1∼10^-2Ω·㎝ and the transmittance of 95%.

비페닐 디메틸 디카르복실레이트(DDB)의 용출향상을 위한 고체분산체의 제조 및 평가

이정우,김정수,이계원,지웅길 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-

Biphenyl dimethyl dicarboxylate(DDB) is synthetic compound derived from Schizandrin C, a component of Fructus Schizandrae. DDB is currently employed as an agent against virally induced hepatic injury and has been found to be effective in improving liver function and symptoms of patients with chronic viral hepatitis. But, DDB is practically water-insoluble and its dissolution rate is extremely low, resulting in very low bioavailability(20-30%). To improve the dissolution rate of DDB, solid dispersion particles of DDB were prepared with two different types of hydrophilic polymers such as Eudragit E100, hydroxypropylmethylcellulose(HPMC) by spray drying method. Properties of solid dispersions were characterized by DSC and PXRD. DDB tablets were prepared by compressing the powder mixtures composed of raw DDB, solid dispersions, lactose and Avicel PH102, magnesium stearate. At ratio above 1:3(w/w), DDB was amorphous state within DDB-Eudragit E100 solid dispersion. As the weight ratio of Eudragit E100 to DDB was increased, the dissolution rate of DDB-Eudragit E100 solid dispersion tablets was reached 69.37-98.29% after 30min. But, it rapidly decreased to 28.34-45.98% after 2hour due to precipitation of DDB. However, in case of 1:3:1 DDB-Eudragit E100-HPMC solid dispersion tablet containing HPMC, dissolution rate was reached 97.47% after 2hour without decrease of dissolution rate. From overall findings, DDB formulation containing Eudargit E100 and HPMC solid dispersion could be used to remarkably improve the dissolution rate in dosage form of tablets.

양측성 Warthin씨 종양 치험례

김상규,우길상,김진수,변기정 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

Warthin's tumor is a bunign neoplasm that occurs almost exclusively in the parotid gland. The tumor most frequently occurs in the tail of the parotid near the angle of the mandible. One unique feature of Warthin's tumor is the tendency of bilateral occurrence, which has been noted in 4 to 7 percent of cases. Most of these bilateral tumors do not occur simultaneously but are metachronous(occurring at different times). Histologically the tumor is composed of a mixture of ductal epithelium and a lymphoid stroma. Surgical removal is the treatment of choic for patients with Warthin's tumor. Warthin's tumor seldom recurs after removal. In our department, a 50-year old man, diagnosed as Warthin's tumor by fine needle aspiration biopsy, was treated by superficial parotidectomy on right side and subtotal parotidectomy on left side. For the treatment of Warthin's tumor, correct pretreatment diagnosis and the selection of appropriate surgical methods are important. We present a case report of the bilateral Warthin's tumor with the review of literatures.

초등학생들의 물질 개념에 관한 연구

김석중,남철우,김정길,송판섭,한광래,최도성,김숙희 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

Over the past 20 years, research on students' conceptual development of science concepts has begun to inform educators about the roles that prior knowledge and experiences play in the development of conceptual understandings. Whereas many initial studies focused on identifying and cataloging naive of misconceptions, more recent research has focused on how student concept development research can inform teaching practice and curriculum design. In this study, we investigated children's (ages 11∼12) spontaneously constructed or naive understanding of the particulate nature of matter prior to any formal instruction in the domain. Thirty students were interviewed concerning their understanding of the macroscopic and microscopic properties of the states of matter(solid, liquid, and gas), as well as their macro/microscopic understanding of phase changes and dissolving. Children expressed ideas about states of matter which were categorized as macrocontinuous, macroparticulate, or microparticulate. Furthermore, a substantial number of the children provided explanations of properties and processes which were consistent with those beliefs. These children's beliefs about matter were not fully and consistently developed across the spectrum of substances from continuous solids to particulate solids to liquids to gases. We speculate that children first develop local frameworks particular to different classes of substances and then slowly expand these frameworks to include a wide range of substances and their properties as well as such processes as melting and freezing.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.