낙동강 원수의 생물활성탄 및 오존처리공정에 따른 수질 변화 및 폴리오바이러스의 제거특성

정은영,박홍기,이유정,정종문,정미은,홍용기,장경립,Jung Eun-Young,Park Hong-Ki,Lee You-Jung,Jung Jong-moon,Jung Mi-Eun,Hong Yong-Ki,Jang Kyoung-Lib 한국생명과학회 2005 생명과학회지 Vol.15 No.5

오존처리는 음용수중의 유해한 미생물을 소독하는 처리기술로 주로 이용되고 있으며, 생물활성탄 처리기술 역시 오염물질 제거를 포함한 음용수 제조과정에 여러 가지 이점을 제공한다. 이글의 병행처리는 원수중의 여러 물질을 제거하는데 효과적인 공정으로 간주되고 있다. 본 연구에서는 낙동강 하류의 매리취수장 원수를 사용하여 생물활성탄 정수처리공정 및 오존에 의한 수질 변화와 함께 폴리오바이러스 제거 효율을 조사하였다. 수질인자들은 BAC 여과를 거치면서 $NH^{+}_{4}-N$등을 포함한 모든 항목들은 거의 제거되는 것으로 나타났다. Pilot-plant를 이용한 정수처리공정별 폴리오바이러스 제거실험에서는 전오존 접촉에 의해 $96.8\%$, 침전단계에서$99.3\%$, 여과단계에서 $99.6\%$의 바이러스가 제거되었으며, 후오존을 거친 BAC 여과수 시료에서는 세포배양법과 ICC-PCR 방법에서 바이러스가 $100\%$ 제거되어짐을 확인할 수 있었다. 오존농도에 의한 폴리오바이러스 제거실험 결과 0.4mg/1에서 5분간 접촉되었을 때는 약 $61.1\%$ 이상이, 0.8mg/1에서 10분 이상 접촉시킨 후에는 바이러스의 $100\% $가 불활성화 되어졌음을 알 수 있었다. Ozonation is a disinfection technique of harmful mi-crobes commonly used in the treatment of drinking water. And Biological Activated Carbon (BAC) treatment also provides numerous benefits for drinking water utilities, including removal of micro- pollutants, improved treatment processes. The multiful-stage ozonation and BAC play roles as effective methods for removing several materials in raw water. Water quality variation in Nak dong river and the removal efficiency of viruses by ozonation-BAC process were investigated on pilot scale. During the period of survey, most of water quality parameters including $NH_{4}^{+}-N$ were highly improved after passing through the BAC. The removal efficiency of poliovirus type III in water treatment process using pilot-plant,$ 99.6\% $ of viruses were removed by pre-ozonation, sedimentation and sand filteration process, $ 100\% $ were removed after in BAC filteration step. In the removal survey of viruses by ozonation, ap-proximately $ 61.1\% $ or polioviruses were inactivated by ozone of 0.4 mg/l within 5 min. and $ 100\% $ were inactivated by ozone of 0.8 mg/l over 10 min.

Capsaicin에 의한 간암세포주의 Apoptosis 유도

정미영,강혜정,문애리 德成女子大學校 藥學硏究所 2000 藥學論文誌 Vol.11 No.1

Hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. In the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in SK-Hep-1 hepatocellular carcinoma cells. Treatment of capsaicin inhibited growth of SK-Hep-1 cells in a concentration-dependent manner. The inhibitory effect of capsaicin on SK-Hep-1 cell growth was mainly due to the induction of apoptosis as evidenced by DNA fragmentation and nuclear condensation. Furthermore, capsaicin prominently reduced the ratio of anti-apoptotic Bcl-2 to proapoptotic Bax. We also show that the activity of DEVDase (caspase-3 like) is more likely involved than that of YVADase (caspase-1 like) in capsaicin-induced growth inhibition. These results deminstrate that capsaicin efficiently induced apoptosis in SK-Hep-1 cells through a caspase-3-dependent mechanism, which may contribute to the chemopreventive function for hepatocellular carcinoma.

돼지고기 드립의 몇 가지 특성과 염지 효과

문윤희,김미숙,정인철 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.4

한국 돼지고기의 등심, 햄 및 삼겹살과 수입 삼겹살에서 유리된 드림의 pH, 수분, 조단백질 및 아미노산 함량을 분석 비교하였다. 또 등심육의 드립에 아질산나트륨 0.02%, 식염 2.0%, 아스코르부산나트륨 0.06%, 설탕 1.0% 및 피로인산나트륨을 0.3%가 되도록 첨가하여 4℃에서 염지하면서 염지효과를 검토하였다. 드립의 pH 및 수분함량은 각각 5.72∼6.05 및 88.05∼90.85%로 부위별로 큰 차이가 없었다. 국내산 돼지고기 등심, 햄, 삼겹살과 수입삼겹살의 조단백질 함량은 각각 11.07, 8.85, 8.76 및 8.13%이었고, 이것을 수분함량과 합하면 99% 정도를 차지하였다. 등심육 드립의 유리아미노산 총량은 다른 부위육보다 많았으며, 부위에 관계없이 glutamic acid, proline, glycine, alanine 및 lysine이 많이 함유되어 있었다. 드립을 염지하였을 때에 아질산염 첨가구가 무첨가구보다 pH 상승 정도가 컸고, 미생물 증식이 느렸으며, 아질산근 잔존량은 염지 3일째부터 현저히 감소되었다. 또 염지기간중 유리아미노산 총량은 증가되었다. This study was carried out to some properties and curring effect of drip obtained from frozen-thawed pork meat. The drip was prepared from frozen domestic pork loin, ham, belly and imported belly by thawing process at 4℃. Moisture content and pH value of drips were 88.05∼90.85% and 5.72∼6.05 and do not show significant differences between each samples. Protein contents were 11.07, 8.85, 8.76 and 8.13% in the drips from domestic pork loin, ham, belly and imported belly, respectively. Approximately 99% of the drip were constituted with moisture and protein in any part of domestic pork and imported belly. Glutamic acid, proline, glycine, alanine and lysine were the predominant amino acid in the drips. Curing process of the drip by nitrite increased the pH value and total amino acid content. The residual nitrite decreased during the period of curing, and total plate counts in drip with nitrite did not reach 1×10 exp (5) CFU/g until 7 days.

발열을 동반한 호중구감소증에서 Cefepime 단독투여와 Ceftazidime 및 Tobramycin 병합투여의 효능비교

정현욱,채제욱,강미라,양정채,문치숙,기현균,장현하,오원섭,김기현,백경란,이남용,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 국내에서 발열을 동반한 호중구감소증이 있는 악성 종양환자에게 경험적 항생제로 베타락탐계 항생제와 아미노배당체의 병합요법의 사용이 일반적이다. Cefepime은 광범위 항균 작용을 가지고 있어, 그람 음성균 뿐만 아니라 그람 양성균에 대해서도 우수한 효과를 나타낸다. 재료 및 방법: 발열을 동반한 호중구감소증이 있는 악성 종양환자를 대상으로 무작위, 공개, 비교 연구를 시행하였다. 대상 환자를 무작위로 cefepime 단독요법군과 ceftazidime 및 tobramycin 병합요법군으로 나누어 투여하고 각각의 임상적 효능과 안전성을 비교하였다. 구강및 인후 점막염이 있는 환자에서 분리된 녹색 연쇄알 구균에 대한 항생제 내성 정도를 조사하였다. 결과 : 대상환자 89명 중 CA 투여군이 48예(53.9%), CT 투여군이 41예(46.1%)이었다. 발열의 유형별로 MDI는 18예(20.2%), CDI는 9예(10.1%), UF는 62예(69.7%)로 두 군 간에 차이가 없었다. CA 투여군과 CT 투여군의 임상적 호전률은 시험약 투여 후 2-4일째 각각 91.7%, 85.4% (P=0.31), 치료 종료 시 각각 91.7%, 100% (P=0.15)로 두 군간에 유의한 차이가 없었다. 치료 종료 시 CA 투여군과 CT 투여군의 세균학적 소실률은 모두 100%로 두 군간의 유의한 차이가 없었다(P=0.78). 점막염이 있는 환자로부터 녹색 연쇄알 구균이 분리된 경우는 25예(28.1%)이었으며, 분리된 녹색 연쇄알 구균은 penicillin, ceftriaxone, cefepime, vancomycin에 모두 감수성을 보였다. 약제 관련 이상 반응의 발생 빈도도 두 군간에 유의한 차이가 없었다. 결론 : 발열을 동반한 호중구감소증이 있는 악성 종양환자의 경험적 항생제로서 cefepime 단독요법은 ceftazidime 및 아미노배당체의 병합요법만큼 효과적이고 안전하였다. Background : Broad-spectrum antibiotic therapy has been recommended as an empirical regimen in cancer patients with febrile neutropenia. Cefepime is a fourth generation cephalosporin with good activity against both gram-positive cocci and gram-negative bacilli. Materials and Methods : To compare the efficacy and safety of cefepime alone with ceftazidime plus tobramycin as empirical regimen for adult cancer patients with febrile neutropenia, a randomized, open label, comparative trial was performed. If the patient showed clinical improvent 72 hours, antibiotic could be changed to oral ciprofloxacin. Clinical and microbiological responses were determined at 72 hours and at the end of therapy. To investigate the antimicrobial resistance of viridans streptococci, swab cultures were obtained from throat in all enrolled patients and antimicrobial susceptibility tests were performed by using microdilution method according to the NCCLS. Results : A total of 89 patients were enrolled. Forty-eight patients received cefepime alone (CA), and 41 patients received ceftazidime plus tobramycin (CT). Demographic and baseline clinical characteristics were similar in both groups (P>0.05). The initial clinical success rate at day 2-4 in group CA (91.7%) was similar with that in CT group (85.4%) (P=0.31). At the end of therapy, the final clinical success rate in CA group (91.7%) was similar to that in CT group (100%) (P=0.15). In 18 patients, with microbiologically defined infections, the eradication rate was 100% in both groups. Adverse events including liver dysfunction (21.3%) and renal dysfunction (2.2%), were similar in both groups (P=0.87). Viridans streptococci were isolated from the throat cultures in 25 cases, and all of these strains were susceptible to penicillin (MIC_(90) 0.12 ㎍/mL), cefepime (1 ㎍/mL), and vancomycin (0.12 ㎍/mL). Conclusion : Efficacy and safety of cefepime monotherapy was comparable to the combination of ceftazidime and tobramycin. It could be used as an alternative empirical regimen for treating cancer patients with febrile neutropenia.

상업 스포츠 센터의 문화적 특성과 직무만족의 관계

문정옥,최미리,이양출 仁荷大學校 스포츠科學硏究所 2001 論文集 Vol.13 No.-

The purpose of this study was to examine the relationship between cultural characteristics and job satisfaction of the commercial sport center. In order to achieve this purpose, the data were collected from 278 male/female employees who worked at the commercial sport centers which were located in Seoul and Incheon City. The statistical methods for the analysis of this study were multiple regression analysis. The conclusions of this study were as follows: First, school career and job career were the effective variables on the job satisfaction of the employees. Second, the group culture and the hierarchical culture were the effective variables on the job satisfaction of the employees. Third, the relation-centered leadership behavior was the effective variable on the cultural characteristics of the commercial sport center.

綜合病院 外來訪問 老人의 健康診斷 結果

정문숙,김미예,정귀옥 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

본 연구는 1990년 7월 10일부터 9월 10일가지 2개월 동안 대구시에 소재하는 일·이차 동시진료기관인 대구의료원 외래 7개과를 방문한 65세 이상 132명의 노인을 대상으로 노인의 일상생활 수행능력, 호소증상, 주진단명, 신경외과, 안과와 치과의 3개과 특별진찰 결과 및 활력증상, 임상검사 결과 등을 면담, 관찰, 검사 및 측정에 의해 조사한 결과는 다음과 같다. 노인의 일상생활 수행시 가장 의존율이 높았던 활동은 보행하기로 13.6%이었으며, 보행, 의복착용, 식사, 목욕 및 화장실 사용등 5가지 일상활동 중 남의 도움을 한가지 이상 받아야 하는 노인의 비율이 15.2%이었다. 병원 외래 7개과중 노인이 가장 많이 방문하는 과는 내과로 49.2%를 차지하였고 남자노인은 신경외과 정형외과 순으로 여자노인은 안과 정형외과 순이었는데 특히 남자노인에 비해 일반외과 방문율이 낮았다. 주로 호소하는 건강문제는 관절통 및 신경통이 24.2%로 가장 많았고 남자노인은 호흡곤란, 불안의 순이었으며, 여자노인은 요통, 호흡곤란 순이었다. 주 진단명은 소화기 질환이 16.7%로 가장 많았고 전염성 질환인 폐결핵이 12.9%로 나타났으며 노인성 백내장이 12.1%이었다. 또 비교적 노화과정에서 나타날 수 있다고 보는 퇴행성 척주증, 퇴행성 관절염, 골다공증 및 노인성 백내장이 전체 질환의 24.2%를 차지했다. 고혈압과 저혈압 증상 노인이 55.3%로 과반수이었으며, 남자노인이 여자노인보다 고혈압군이 더 높은 비율이었다. 임상소견으로 혈색소 검사에서 빈혈을 보인 노인이 26.5%이었고, 뇨당과 뇨단백의 양성율이 각각 10.6% 및 6.8%이었으며 노인의 21.2%가 청력에 이상이 있었다. 대상노인의 신경외과 진찰결과는 요통이 65.9%, 안과에서는 백내장이 90.9%, 그리고 치과 검사에서는 치주질환이 72.7%로 나타났으며 75세 이상 노인 34명은 모두 백내장으로 나타났다. 외래방문후 진찰결과 입원율은 27.3%(36명)이었으며 남자노인이 38.2%로 여자노인의 19.5%보다 높은 비율이었다. The purpose of this study was to assess health problem complained by the aged who visited OPD of one general hospital and to abtain the basic data which were necessary for the improvement of their health status by testing such as vital sign checks and clinical laboratory test and examination. Also by interviewing with 132 patients who live in Taegu and near cities the data was collected from July 10, to September 10, 1990. The results were as follows; In case of ADL, the dependent rate by each item was shown that the walking from 13.6% which was the highest percentage. It was shown that the rate of the aged visited to internal medicine department was 49.2% of the subjects and among them 24.2% was mainly suffered from a joint pain and body pain which was the highest rank and the next was backache. Chief diagnosis of the aged with complaints was gastrointestinal disease in 16.7% which was the highest rank, the next was pulmonary tbc(12.9%) and third was senile cataract(12.1%). The mean distribution of hypertensive disorder and hypotensive disorder was 55.3% in systolic and in diastolic BP. In case of clinical labolatory test, the incidence rate of anemia was shown as 26.5%, and positive rate of urine-sugar was shown as 10.6% and protein uria was shown as 6.8% of subjects. After physical examination and testing in neurosurgery, the incidence rate of backache was shown 65-9% in optimalmic unit the incidence rate of senile cataract was shown as 90.9%(almost subjects). The rate of hospitalization after diagnosis was shown as 27.3%.

플라비아닌산을 이용한 클로르프마진의 용매추출 분광광도법

정문모,남수자,이미나,이지연,허문회,안문규 慶星大學校 1998 論文集 Vol.19 No.2

Q 열에 의한 감염성 심내막염 2예

문수연,최영실,박미연,이정아,정미경,정혜숙,정두련,송재훈,백경란 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

Q fever is a zoonosis caused by Coxiella burnetii, Presenting as acute and chronic illness and it has been reported worldwide. Acute Q fever is usually asymptomatic or mild and self-limiting, but infective endocarditis is one of the most serious complications of chronic Q fever and can be fatal. Known risk factors for Q fever endocarditis are valvular heart disease, immunocompromised hosts, and pregnancy. There have been some reports on Q fever in Korea but there exists no report on Q fever endocarditis. We have experienced 2 cases of Q fever with underlying valvular heart disease; both Patients came to the hospital for evaluation of prolonged fever. Although Q fever and Q fever endocarditis are rare in Korea, Q fever endocarditis should be considered in the differential diagnosis of patient with infective endocarditis when causative microorganism cannot be identified.

RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

Helicobacter pyloir 양성 소화성 궤양에서 lansoprazole 포함 삼제요법의 제균율 및 제균판정에 있어서 요소호기검사의 유용성

정혜경,곽재진,유민아,배기선,권정미,이종수,김도영,문일환 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : 본 연구는 Helicobacter pylori (이하 H. polyri) 양성인 소화성 궤양 환자에서 lansoprazole 포함 삼제요법의 제균율을 알아보고, 제균 치료 후 제균 판정에 있어서 요소 호기 검사(Urea breath test, 이하 UBT) 및 신속요소분해(rapid urease test, 이하 RUT test)와의 일치율에 대하여 알아보고자 하였다. 방법 : 상부위장관내시경 검사에서 H. polyri 양성인 소화성 궤양환자에서 lansoprazole 60㎎+amoxicillin 2g+clarithromycin 1g의 약제를 2회 분복하여 1주간 복용하는 삼제요법을 실시하였다. 최소 4주후 추적 상부위장관내시경을 실시하였고, 전정부와 체부에서 각각 RUT를 실시하였으며 UBT(5분, 20분)를 시행하였다. 결과 : 대상환자는 총 46명으로 남자 34명(48±13세), 여자 12명(53±14세)이었다. LAC 삼제요법의 제균율은 40/46명(87.0%)이었고, 궤양의 치유율도 42/46명(91.3%)이었다. RUT와 20분 UBT 결과가 모두 음성이었던 경우는 41예, 모두 양성이었던 경우는 4예로 RUT와 UBT는 97.8% (45/46)의 일치율을 보였고, 1예는 UBT는 음성이었으나 체부에서 시행한 RUT가 양성이었다. 5분 UBT 검사가 양성이었던 예는 14/34 (41.2%) 이었고, 이 14예 중 12예(85.7%)는 20분 UBT 검사 음성, RUT 음성이었다. 결론 : Lansoprazole과 amoxicillin, clarithromycin 1주일 병합요법은 H. polyri 양성인 소화성 궤양 환자에서 87.0%의 제균율을 나타내어 우수한 효과를 보였고, 요소호기검사는 제균 치료 후 제균 판정에 유용한 비침습적인 방법임을 확인할 수 있었다. Background : There are only a few studies on Helicobacter pylori (H. pylori) for its eradication rates of lansoprazole-based triple therapy in Korea, and the results are controversial. Therefore, we undertook to investigate the eradication rate of lansoprazole-based triple therapy, and compare the concordance rate of urea breath test (UBT) and rapid urease test (RUT) in evaluating H. pylori eradication. Methods : Patients with acute peptic ulcer who were H. pylori-positive were recruited by prospective, consecutive manner. They received lansoprazole 30 ㎎ b.d., amoxicillin 1 g b.d. and clarithromycin 500 ㎎ b.d. for 1 week. Upper endoscopy was performed after 4 weeks to check for ulcer healing, and UBT and RUT were performed to evaluate H. pylori eradication status. Results : A total of 46 patients were recruited, and they were all compliant. H. pylori eradication rate was 87.0% (40/46) and ulcer healing rate was 91.3% (42/46). Forty one patients showed negative in both UBT and RUT, and 4 patients revealed positive in both tests, therefore, the concordance rate of UBT and RUT was 97.8% (45/46). Conclusion : Our study showed that 1-week lansoprazole-based triple therapy was effective in H. pylori eradication and ulcer healing. UBT can be an effective, noninvasive method for evaluating H. pylori status after H. pylori eradication.