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Julian A Ferreras,Ryu, Jae-Sang,Federico Di Lello,Derek S Tan,Luis E N Quadri 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16
Mycobacterium tuverculosis and Yersinia pestis, the causative agents of tuberculosis and plague respectively are pathogens with serious ongoing impact on global public health^(1,2) and potential use as agents of bioterrorism^(3). Both pathogens have iron acquisition systems based on siderphores secreted iron-chelating compounds with extremey high Fe^(3+)affinity(4,5) Several lines of evidence suggest that siderophores have a critical role in bacterial iron acquisition inside the human host^(6-9) where the free iron concentration is well bellow that required for bacterial growth and virulence ^(10) Thus, siderophore biosynthesis is an attractive target in the development of new antibiotics to treat tuberculosis and plague^(2,5,8,11). In particular, such drugs alone or as part of combination therapies could provide a valuable new line of defense against intractable muliple-drug-resistant infections Here we report the design synthesis and domain salicylation enzymes required for siderophore biosynthesis siderophore biosynthesis and growth of M. tuberculosis and Y, pestis under iron-limiting conditions.
Ferreras, Julian A.,Stirrett, Karen L.,Lu, Xuequan,Ryu, Jae-Sang,Soll, Clifford E.,Tan, Derek S.,Quadri, Luis E.N. 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.18
Phenolic glycolipids (PGLs) are polyketide-derived virulence factors produced by Mycobacterium tuber-culosis, M. leprae, and other mycobacterial pathogens. We have combined bioinformatic, genetic, biochemical, and chemical biology approaches to illuminate the mechanism of chain initiation required for assembly of the p-hydroxyphenyl-polyketide moiety of PGLs. Our studies have led to the identification of a stand-alone, didomain initiation module, FadD22, comprised of a p-hydroxybenzoic acid adenylation domain and an aroyl carrier protein domain. FadD22 forms an acyl-S-enzyme covalent intermediate in the p-hydroxyphenyl-polyketide chain assembly line. We also used this information to develop a small-molecule inhibitor of PGL biosynthesis. Overall, these studies provide insights into the biosynthesis of an important group of small-molecule mycobacterial virulence factors and support the feasibility of targeting PGL biosynthesis to develop new drugs to treat mycobacterial infections.