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Toxicity testing of wastewater and inter-laboratory comparison based on Lemna minor root elongation
Jihae Park(박지혜),Eun-Jin Yoo(유은진),Kisik Shin(신기식),Soyeon Choi(최소연),Hojun Lee(이호준),Geonhee Kim(김건희),Stephen Depuydt(스테픈 데퓨잇),Jonas De Sager(요나스 데 세거),Taejun Han(한태준) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
The Lemna minor root regrowth test method is a toxicity test performed with very simple equipment and only one L. minor plant (two- to three-frond) in each culture well, cutting the roots before exposure and measuring the regrowth of the newly developed roots after three days. As a substrate for laboratory toxicity assays, L. minor has the advantages of small size, structural simplicity and rapid growth. Compared to the three internationally standardized methods, this bioassay is faster, simpler, more convenient and cheaper. It can be applied to a variety of sample types, including (a) individual chemicals, commercial products or known mixtures of chemicals, (b) industrial or urban freshwater effluents, elutriates or leachates and (c) surface freshwater or receiving waters. The test can be used alone or as part of a test battery to determine and monitor the toxic effects of individual substances or complex mixtures that may be harmful to aquatic life in freshwater ecosystems, and as a weight-of-evidence approach to determine the need for controlling discharges and setting effluent standards.