http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Jinfen Wang (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
PEG-mediated transduction of rAAV as a platform for spatially confined and efficient gene delivery
Liang Zou,Jinfen Wang,Ying Fang,Huihui Tian 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Background: Recombinant adeno-associated viruses (rAAV) are commonly used vectors for gene delivery in both basic neuroscience and clinical applications due to their nonpathogenic, minimally immunogenic, and sustained expression properties. However, several challenges remain for the wide-scale rAAV applications, including poor infection of many clinically important cell lines, insufficient expression at low titers, and diffusive transduction in vivo. Methods: In this work, PEG, which is a safe and non-toxic polymer of ethylene oxide monomer, was applied as an auxiliary transduction agent to improve the expression of rAAV. In detail, a small dose of PEG was added into the rAAV solution for the transgene expression in cell lines in vitro, and in the central nervous system (CNS) in vivo. The biocompatibility of PEG enhancer was assessed by characterizing the immune responses, cell morphology, cell tropism of rAAV, neuronal apoptosis, as well as motor function of animals. Results: The results show that small dose of PEG additive can effectively improve the gene expression characteristics of rAAV both in vitro and in vivo. Specifically, the PEG additive allows efficient transgene expression in cell lines that are difficult to be transfected with rAAV alone. In vivo studies show that the PEG additive can promote a spatially confined and efficient transgene expression of low-titer rAAV in the brain over long terms. In addition, no obvious side effects of PEG were observed on CNS in the biocompatibility studies. Conclusions: This spatially confined and efficient transduction method can facilitate the applications of rAAV in fundamental research, especially in the precise dissection of neural circuits, and also improve the capabilities of rAAV in the treatment of neurological diseases which originate from the disorders of small nuclei in the brain.
-
Polymer nanofiber network reinforced gold electrode array for neural activity recording
Siting Yang,Ke Xu,Shouliang Guan,Liang Zou,Lei Gao,Jinfen Wang,Huihui Tian,Hui Li,Ying Fang,Hongbian Li 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.2
Flexible and stretchable neural electrodes are promising tools for high-fidelity interfacing with soft and curvilinear brainsurface. Here, we describe a flexible and stretchable neural electrode array that consists of polyacrylonitrile (PAN) nanofibernetwork reinforced gold (Au) film electrodes. Under stretching, the interweaving PAN nanofibers effectively terminate theformation of propagating cracks in the Au films and thus enable the formation of a dynamically stable electrode-tissue interface. Moreover, the PAN nanofibers increase the surface roughness and active surface areas of the Au electrodes, leading toreduced electrochemical impedance and improved signal-to-noise ratio. As a result, PAN nanofiber network reinforced Auelectrode arrays can allow for reliable in vivo multichannel recording of epileptiform activities in rats.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
