재조합 균주 Bacillus subtilis LKS88에 의한 Streptomyces albus KSM-35 Amylase의 생산조건

최원진,유도진,이재우,소명환,김영배 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

Streptomyces albus KSM-35의 amylase의 유전자를 Bacillus subtilis LKS88에서 발현시킨 균주를 사용하여 amylase의 생산에 미치는 몇가지 요인들을 조사하였다. 본 실험에 사용한 탄소원들중에서 sodium citrate와 왕겨의 효소 생산량이 가장 높았고, 이들을 1:1의 비율로 혼합하여 첨가하였을 때 효소의 생산량은 대조군으로 사용한 가용성 전분에 비하여 20배 이상 증가하였다. 질소원으로 대두박을 사용하였을 경우 yeast extract의 첨가량을 줄이면서 효소의 생산량을 증가시켰다. 배양을 위한 초기 pH를 60으로 조절하고 SDS를 0.01%첨가하였을 때 대조구조보다 높은 효소 생산량을 나타내었다. Bacillus subtilis LKS88 (pASA240)을 sodium citrate와 왕겨 각각 1.5%, K_2HPO_4 0.66%, yeast extract 0.3%, 대두박 0.7%, SDS 0.01% 및 초기 pH를 6.0으로 조절한 배지에서 37℃에서 배양시 최대 효소 활성은 배양 후 36시간에서 나타났으며 이때 효소 활성은 56.6 U/ml이었다. The effects of culture conditions on the production of amylase expressed by Bacillus subtilis LKS88 with a cloned gene from Streptomyces albus KSM-35 were investigated The production of amylase was increased significantly by using sodium citrate and rice hull as a carbon source. In addition, the use of a mixture of sodium citrate and rice hull(1:1) resulted in increase of enzyme production by 20-fold when compared to that of soluble starch. The soybean meal as the nitrogen source could be partially replaced with yeast extract without changing the enzyme production yield. The amylase production was also increased by adjusting initial pH to 6.0 or by adding 0.01% SDS. Maximum amylase production was observed in the medium containing 1.5% sodium citrate, 1.5% rice hull, 0.7% soybean meal, 0.3% yeast extract, 0.66% K_2HPO_4, 0.05% MgSO_4·7H_2O, 0.08% CaCl_2·2H_2O, 0.01% SDS with initial pH of 6.0. The maximum yield of amylase reached 56.6 U/ml when B. subtilis LKS88(pASA 240) was cultured at 37℃ for 36 hr.

안검과 수정체 난시를 고려한 도난시 도수 결정

李原鎭,成德鏞,金在道 대구산업정보대학 1994 논문집 Vol.8 No.-

This study is investigated the subject refract examination about relation astigmatism power and axis with the eyelid and lens astigmatism. Astigmatism is generally accredited as being one of the major causes of ocular asthenopia. This may be due to the requirement for constant accommodation in an attempt to place the cicle of least confusion on the retina and to subsidiary actions such as contraction of the eyelids to secure stenopaic slit effect, the narrowing of the pupil aperture to reduce aberration. Frontal and temporal headaches, tearing, smarting and eye-strain are all common to astigmia. In particular, inverse astigmatism produced more asthenopia than direct astigmatism. Because the contraction of the eyelids to stenopaic slit effect and the axis of lens astigmatism are connected with astigmatism axis. Therefore, the astigmatism axis and power may be considered the eyelids and lens astigmatism axis.

완전 포상기태 이후 발생한 지속성 융모질환의 치료경험 2례.

이진희,류은석,김윤숙,김종수,최승도,선우재근,배동한 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Persistent tumor develops in approximately 20% of patients with a complete mole, and chemotherapy is required to achieve remission. Following evacuation of hydatidiform mole, careful hCG monitoring is mandatory since it is the most reliable and sensitive method for the early detection of gestational trophoblastic disease. In carefully selected patients in whom the risk of developing gestational trophoblastic disease is significant or when the availability of hCG testing is suboptimal, chemoprophylaxis has been shown to decrease the risk of gestational trophoblastic tumor. We report here two cases of patient, 16-year-old and 45-year-old women who experienced unusual course after the evacuation of a complete mole and markedly elevated serum β-hCG levels. The patient developed persistent gestational trophoblastic disease and were successfully treated with EMA-CO and hysterectomy.

한국 학생운동사와 그 사회적 의미

유도진,김재경 慶熙大學校 1994 論文集 Vol.23 No.-

Cellulose Nitrate의 알파粒子飛跡特性

田載植,黃善泰,都珍悅 대한방사선 방어학회 1984 방사선방어학회지 Vol.9 No.2

Cellulose nitrate의 α粒子飛跡特性, 特히 化學腐蝕條件에 따른 α 粒子의 飛跡直徑을 中心으로 硏究하였다. Cellulose nitrate의 KOH 溶液腐蝕때에는 同一한 腐蝕條件下에서 NaOH 경우보다 α 飛跡直徑이 約 3倍 크게 나타났다. 그밖에 腐蝕時間 및 粒子의 에너지에 따른 腐蝕直徑의 데이타를 얻었고, α 粒子의 照射條件이 같을 때 훌루언스率(fluence rate)이 腐蝕時間에 無關함을 알았다. A study on the characteristics of α-particle track on cellulose nitrate film was carried out with a particular emphasis on the dependence of track diameter upon chemical etching condition. The track diameters etched in KOH solution appeared to be, on average, three times larger than those etched in NaOH under the same etching condition. The relationship between the track diameters and both etching time and the energy of incident α-particles was also investigated. It is shown that the particle fluence rate is fairly independent of etching time as far as the condition of irradiation remains unchanged.

도토리澱粉이 흰쥐의 脂質代謝에 미치는 影響

崔到点,金載營,高鎭福 新羅大學校 1984 論文集 Vol.16 No.-

In an attempt to observe the lipid metabolism of acorn starch, five different diets were made: one rice diet (control group) and four diets in which acorn starch was mixed with rice at the rate of 10%, 20%, 30% and 40% respectively. These five diets controlled in such ways are the same level in calorie and protein content. The acorn starch used was cleared from tannin by being heated 20 hours. These diets were each fed to different groups of male albino rats for 6 weeks, during which various items were measured on these rats such as total lipid, total cholesterol, triglyceride, phospholipid and HDL-cholesterol contents. The results obtained through this study are summarized as follow: The content of total lipid in liver of each group was lower in the 2nd week, and that of 30% acorn starch group was lower in the 4th week, and that of 20% acorn starch group was lower in the 6th week, compared with control group. The content of total cholesterol in liver of each group except 10% acorn starch group was lower than that of the control group in the 2nd week, but in the 4th and the 6th week, there was no particular difference in content between each group and the control group. The centents of total lipid, triglyceride, phospholipid, total cholesterol and HDL-cholesterol in serum are almost the same between each group and the control group during each period of feeding. Therefore acorn starch diets mixed with rice at the rate of 10%, 20%, 30% and 40% had little effect on the serum lipid contents.

포러스 콘크리트를 利用한 보도용 콘크리트판 적용에 關한 實驗的 硏究

김재은,윤상혁,김우재,김성식,이영도,정상진 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

Although porous concrete is recently used as a pavement at a parking lot, a public square and a bicycle road, we use without enough examining. Most the maintenance of quality for porous concrete is only dealt with strength and color tone. Also, there is not yet enough fundamental data about dynamical properties for strength, rate of void and water permeability, even when it is applied to at the scene, it is been a matter. Accordingly in this study analyzed influences through the dynamical properties, combination, and time to vibration. After the analyzation, they investigated peculiar dynamics which are strength of porous concrete, rate of void, and water permeability follow the rate of increase on an admixture making pozzolan reaction. This is purpose to improve capacity of the sidewalk permeable concrete and development of the economical technique for pavement.

정신분열병 환자에서 자기공명영상 구획화를 이용한 전두엽 하위구조와 구조적 이상 관찰

김재진,권준수,강도형,윤탁,한문희,장기현 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.6

연구목적 : 정신분열병 환자를 대상으로 한 구조적 뇌영상 연구들은 국소적 전두피질(prefrontal cortex)의 이상에 대하여 상당히 다양한 결과들을 보고하였다. 본 연구는 정확한 해부학적 구획화를 바탕으로 한 관심영역 체계를 통해 정신분열병에서 전두피질의 구조적 이상 유무를 탐색하고자 시행되었다. 방 법 : 12명의 정신분열병 환자와 12명의 나이 및 성별 분포가 동일한 정상인들을 대상으로 뇌 자기공명 영상을 획득한 후, 지형학적 표지물을 이용하여 8개의 전두엽 하위구조 구획 작업을 실시하였다. 구획된 전두엽 하위구조들의 용적을 측정하여 두 군간에 차이가 있는가 관찰하였고, 하위구조 용적과 정신분열 증상의 심각도 간의 상관관계를 살펴보았다. 결 과 : 상당 수의 전두엽 하위구조들의 용적이 정상인에 비해 정신분열병 환자들에서 작은 경향을 보였고, 특히 우측 부운동영역(supplementary motor area)이 통계적으로 유의하게 작은 용적을 보였다(p<0.003). 또 이러한 전두엽 하위구조들의 용적 감소는 정신분열병 음성 증상의 심각도와 밀접한 상관성을 보였고, 이러한 현상은 우측 상전두이랑(superior frontal gyrus)에서 가장 대표적이었다(γ=-0.704, p=0.01). 결 론 : 이러한 결과는 정신분열병에서 전두엽기능저하의 바탕이 되는 구조적 결함의 존재 가능성을 시사한다. 이러한 전두피질의 구조적 결함은 정신분열병에서의 인지기능결핍과 신경회로의 장애 소견과 연결될 수 있다. Objectives : Although most of the functional and structural neuroimaging studies have reported the association of the that cognitive deficits in patients with schizophrenia with the pathophysiology of the prefrontal cortex, their findings vary considerably. Based on the precise parcellation methods, this study was designed to investigate substructural abnormalities of the prefrontal cortex in patients with schizophrenia. Methods : The subjects consisted of twelve patients with schizophrenia and 12 age- and sex-matched normal volunteers. Magnetic resonance images were obtained in all subjects, and parceled into 8 frontal substructures using the topographic landmarks. The frontal substructural volumes were compared between the two groups, and their correlations with the schizophrenic symptom severity were analysed in the patient group. Results : In the comparison of substructural volumes, most frontal substructures of the patient group tended to be smaller than those of the normal comparison group ; particularly the right supplementary motor area was significantly smaller(p<0.003). Negative symptoms tended to be inversely correlated with the frontal substructural volumes, particularly with the right superior frontal gyral volume(γ=-0.70, p=0.01). Conclusion : These findings suggest the possibility of structural defects related to 'hypofrontality' in schizophrenia. These frontal structural defects may be the basis of neurocognitive deficits and neural circuital dysfunction in schizophrenia.

하악전돌증의 악교정수술 후 연조직 형태변화에 대한 연구

성상진,박현도,김재승,문윤식 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.3

악교정수술에 관한 치료계획은 치료결과에 관한 정확한 예측을 바탕으로 수립되는 것이 바람직하며 이는 심미적인 결과를 얻기 위한 가장 중요한 요소 중에 하나이다. 성인에서 골격성 하악전돌증의 치료는 하악골후퇴술을 이용한 악교정수술법이 주로 이용되는데, 술후 턱끝의 돌출도가 과도하게 감소되어 하악전진이부성형술을 추가로 시행해야 하는 경우 하악골과 턱 부위의 연조직 변화방향이 상이하게 나타나므로 술후 연조직 변화를 정확히 예측하는데 한계가 있다. 본 연구에서는 골격성 하악전돌증을 주소로 악교정수술을 시행한 성인을 대상으로, 상행지시상분할골절단술(BSSRO)만을 시행한 경우(A군) 11명과 하악전진이부성형술(advancement genioplasty)도 같이 시행한 경우(B군) 9명의 경조직과 연조직 변화의 상관관계를 알아보기 위하여, 수술 8개월 이후에 채득된 측모두부계측방사선사진을 비교하여 다음과 같은 결과를 얻었다. 1. E-line에 대하여 상순은 더 돌출되고 하순은 더 후퇴되어 입술의 형태가 개선되었으며,하악전진이부성형술을 병행한 경우 하순의 돌출도는 더 개선되었다. 2. 두 군 모두에서 상순은 후방이동하고 비순각은 증가하였다. 3. 술후 B점에 대한 연조직 POGs점의 변화율은 A군에서 84%, B군에서 66%로 나타났으며 두 군간에 통계적 유의성이 있었다. 4. 경조직과 계측점의 수직변화량은 A군에서 감소하였다. The treatment plan for orthognathic surgery must be based on accurate predictions, and this can be produced the most esthetic results. Treatment of prognathic mandible in adult is usually orthognathic surgery using mandible set back, but mandible with retruded chin point is needed additional chin augmentation. In this case, the directions between mandible and chin point are different therefore, the prediction of soft tissue reactions must be modified. In this study, we materialize the patients who was taken orthognathic surgery due to prognathic mandible, 11each(Group A) was taken only Bilateral Sagittal Sprit Ramus Osteotomy (BSSRO), 9each(Group B) was taken additional advancement genioplasty. The lateral cephalometric radiography taken 8 months later after orthognathic surgery by this patients were used. The results of this study were as follows : 1.The profile of lips was favorable after surgery due to upper lip to E-line became prominent and lower lip to E-line was retruded. 2.In both group, upper lip moved posteriorly and nasolabial angle was increased. 3.The ratio of the soft tissue profile change in POGs point to skeletal B point movement was 84% in group A and 66% in group B, and there was statistical significance between group A and group B. 4.Vertical movement of hard tissue points is decreased in group A.

교육연극을 위한 극본 자료 개발

김재수,최도순,정경미,백혜경,조은주,김선진 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

When teachers are in need of the script of a play during school hours, Teachers could make a drama out of an interesting story. And by doing learning activities that students are also able to replace an interesting story with a drama, Students are able to understand create an original story. For this purposes, this study explains the methods that teachers make a story. And we make a drama out of a story which is korean language textbook for the seventh curriculum (one∼for grade). And we have teachers to use it lesson.