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Ngoc-Viet Nguyen,Jih-Hao Yeh,Chun-Ping Jen 한국바이오칩학회 2018 BioChip Journal Vol.12 No.3
This study proposes a handheld electronics module integrated with the microchip that utilizes dielectrophoretic (DEP) impedance measurement for characterizing of cancerous cell lines. The microchip consists of circle-on-line-shaped interdigitated microelectrodes, which were used for DEP concentration and impedance sensing of cells within a chamber, patterned by standard microfabrication processes. The handheld electronics module was built for measuring impedance of cancerous cells in the microchip. It can provide sinusoidal electrical signals in a large range of frequencies. The analyzed results demonstrate a high-sensitivity impedance measurement. Meanwhile, the linear relationships between the admittance variation and the number of cell were observed for both two human lung cell lines, namely A549 human lung carcinoma cells and MRC-5 human lung epithelial cells. The difference in the slopes of these characteristic lines could be used to distinguish two stages of lung cells. The proposed device is simple to operate, has high sensitivity, inexpensive and portable.